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Protein

Replication factor C small subunit

Gene

rfcS

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 528ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2102-MONOMER.
BRENDAi3.6.4.B8. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C small subunit
Short name:
RFC small subunit
Alternative name(s):
Clamp loader small subunit
afRFC small subunit
Short name:
afRFCsm
Gene namesi
Name:rfcS
Ordered Locus Names:AF_2060
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Replication factor C small subunitPRO_0000135971Add
BLAST

Interactioni

Subunit structurei

Heteropentamer composed of four small subunits (RfcS) and one large subunit (RfcL). Both subunits interact with PCNA.1 Publication

Protein-protein interaction databases

STRINGi224325.AF2060.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 104Combined sources
Helixi16 – 183Combined sources
Helixi23 – 3412Combined sources
Beta strandi41 – 444Combined sources
Helixi51 – 6313Combined sources
Helixi64 – 707Combined sources
Beta strandi71 – 755Combined sources
Helixi82 – 9312Combined sources
Beta strandi98 – 1003Combined sources
Beta strandi104 – 1096Combined sources
Helixi111 – 1133Combined sources
Helixi116 – 12813Combined sources
Turni129 – 1324Combined sources
Beta strandi133 – 1408Combined sources
Helixi142 – 1443Combined sources
Helixi147 – 1504Combined sources
Beta strandi153 – 1575Combined sources
Helixi163 – 17715Combined sources
Helixi183 – 19311Combined sources
Helixi197 – 20913Combined sources
Beta strandi210 – 2123Combined sources
Helixi216 – 2249Combined sources
Helixi228 – 24013Combined sources
Helixi243 – 25614Combined sources
Helixi261 – 27313Combined sources
Helixi281 – 29717Combined sources
Helixi302 – 31514Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CHGX-ray2.10A/B/C/D1-226[»]
2CHQX-ray3.50A/B/C1-319[»]
2CHVX-ray4.00A/B/C/D/E/F1-319[»]
ProteinModelPortaliO28219.
SMRiO28219. Positions 6-318.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28219.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00469. Archaea.
COG0470. LUCA.
KOiK04801.
OMAiPFKVIFL.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01509. RfcS. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR023748. Rep_factor-C_ssu_arc.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

O28219-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENFEIWVEK YRPRTLDEVV GQDEVIQRLK GYVERKNIPH LLFSGPPGTG
60 70 80 90 100
KTATAIALAR DLFGENWRDN FIEMNASDER GIDVVRHKIK EFARTAPIGG
110 120 130 140 150
APFKIIFLDE ADALTADAQA ALRRTMEMYS KSCRFILSCN YVSRIIEPIQ
160 170 180 190 200
SRCAVFRFKP VPKEAMKKRL LEICEKEGVK ITEDGLEALI YISGGDFRKA
210 220 230 240 250
INALQGAAAI GEVVDADTIY QITATARPEE MTELIQTALK GNFMEARELL
260 270 280 290 300
DRLMVEYGMS GEDIVAQLFR EIISMPIKDS LKVQLIDKLG EVDFRLTEGA
310
NERIQLDAYL AYLSTLAKK
Length:319
Mass (Da):35,994
Last modified:January 1, 1998 - v1
Checksum:i0EBD27AE0AD827DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89191.1.
PIRiC69507.
RefSeqiWP_010879552.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89191; AAB89191; AF_2060.
GeneIDi24795809.
KEGGiafu:AF_2060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89191.1.
PIRiC69507.
RefSeqiWP_010879552.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CHGX-ray2.10A/B/C/D1-226[»]
2CHQX-ray3.50A/B/C1-319[»]
2CHVX-ray4.00A/B/C/D/E/F1-319[»]
ProteinModelPortaliO28219.
SMRiO28219. Positions 6-318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89191; AAB89191; AF_2060.
GeneIDi24795809.
KEGGiafu:AF_2060.

Phylogenomic databases

eggNOGiarCOG00469. Archaea.
COG0470. LUCA.
KOiK04801.
OMAiPFKVIFL.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2102-MONOMER.
BRENDAi3.6.4.B8. 414.

Miscellaneous databases

EvolutionaryTraceiO28219.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01509. RfcS. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR027417. P-loop_NTPase.
IPR023748. Rep_factor-C_ssu_arc.
IPR013748. Rep_factorC_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFCS_ARCFU
AccessioniPrimary (citable) accession number: O28219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.