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Protein

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

Gene

flpA

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2129-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
Gene namesi
Name:flpAUniRule annotation
Ordered Locus Names:AF_2087
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi70 – 701T → A: Loss of RNA methyltransferase activity. No effect on affinity for S-adenosyl-L-methionine. 1 Publication
Mutagenesisi88 – 881E → A: Loss of RNA methyltransferase activity. No effect on affinity for S-adenosyl-L-methionine. 1 Publication
Mutagenesisi89 – 891Y → A: Strongly reduced RNA methyltransferase activity. Reduced affinity for S-adenosyl-L-methionine. 1 Publication
Mutagenesisi133 – 1331D → A: Loss of RNA methyltransferase activity. Strongly decreased affinity for S-adenosyl-L-methionine. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Fibrillarin-like rRNA/tRNA 2'-O-methyltransferasePRO_0000148529Add
BLAST

Interactioni

Subunit structurei

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.UniRule annotation2 Publications

Protein-protein interaction databases

IntActiO28192. 1 interaction.
STRINGi224325.AF2087.

Structurei

Secondary structure

1
210
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Beta strandi8 – 114Combined sources
Beta strandi14 – 185Combined sources
Beta strandi24 – 263Combined sources
Beta strandi33 – 353Combined sources
Helixi38 – 403Combined sources
Helixi42 – 487Combined sources
Beta strandi60 – 645Combined sources
Helixi70 – 789Combined sources
Turni79 – 813Combined sources
Beta strandi82 – 876Combined sources
Helixi91 – 10313Combined sources
Beta strandi105 – 1106Combined sources
Helixi117 – 1193Combined sources
Turni120 – 1234Combined sources
Beta strandi127 – 1326Combined sources
Helixi139 – 15012Combined sources
Beta strandi151 – 16212Combined sources
Helixi163 – 1664Combined sources
Helixi172 – 18413Combined sources
Beta strandi187 – 1948Combined sources
Turni196 – 1983Combined sources
Beta strandi202 – 2098Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NT2X-ray2.90A1-210[»]
ProteinModelPortaliO28192.
SMRiO28192. Positions 2-210.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28192.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 712S-adenosyl-L-methionine binding
Regioni88 – 892S-adenosyl-L-methionine binding
Regioni113 – 1142S-adenosyl-L-methionine binding
Regioni133 – 1364S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. Fibrillarin family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00078. Archaea.
COG1889. LUCA.
KOiK04795.
OMAiPVYGEPT.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA. 1 hit.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiSM01206. Fibrillarin. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28192-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKELMRNVYL LDDTLVTKSK YGSHYGEKVF DGYREWVPWR SKLAAMILKG
60 70 80 90 100
HRLKLRGDER VLYLGAASGT TVSHLADIVD EGIIYAVEYS AKPFEKLLEL
110 120 130 140 150
VRERNNIIPL LFDASKPWKY SGIVEKVDLI YQDIAQKNQI EILKANAEFF
160 170 180 190 200
LKEKGEVVIM VKARSIDSTA EPEEVFKSVL KEMEGDFKIV KHGSLMPYHR
210
DHIFIHAYRF
Length:210
Mass (Da):24,327
Last modified:January 1, 1998 - v1
Checksum:i56946F477586EDA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89169.1.
PIRiF69510.
RefSeqiWP_010879579.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89169; AAB89169; AF_2087.
GeneIDi1485314.
KEGGiafu:AF_2087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89169.1.
PIRiF69510.
RefSeqiWP_010879579.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NT2X-ray2.90A1-210[»]
ProteinModelPortaliO28192.
SMRiO28192. Positions 2-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO28192. 1 interaction.
STRINGi224325.AF2087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89169; AAB89169; AF_2087.
GeneIDi1485314.
KEGGiafu:AF_2087.

Phylogenomic databases

eggNOGiarCOG00078. Archaea.
COG1889. LUCA.
KOiK04795.
OMAiPVYGEPT.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2129-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO28192.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA. 1 hit.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SMARTiSM01206. Fibrillarin. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFLPA_ARCFU
AccessioniPrimary (citable) accession number: O28192
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.