Reviewed, UniProtKB/Swiss-Prot O28185 (PTH_ARCFU)

Names and origin

Protein names

Recommended name:
    Peptidyl-tRNA hydrolase
      Short name=PTH
    EC=3.1.1.29

Gene names

Name:	pth
Ordered Locus Names:	AF_2095

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity.
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00628
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PTH2 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 115

115

Peptidyl-tRNA hydrolase HAMAP MF_00628

PRO_0000120288

Secondary structure

1

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115

Helix Strand Turn

Details...

Beta strand

4 – 10

7

Helix

19 – 36

18

Helix

39 – 42

4

Helix

44 – 46

3

Beta strand

48 – 54

7

Helix

58 – 70

13

Beta strand

75 – 78

4

Beta strand

92 – 99

8

Helix

101 – 108

8

Sequences

	Sequence		Length	Mass (Da)
	O28185-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 4DEEC200CF881489	FASTA	115	12,487
	10 20 30 40 50 60 MTLKQVIVVR DDLKLSRGKL AVQVAHAAII GYLKSDSSLR RKWLDEGQKK VVLKVKSLEE 70 80 90 100 110 LLGIKHKAES LGLVTGLVQD AGLTEVPPGT ITAVVIGPDE ERKIDKVTGN LPLLK

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000782 Genomic DNA. Translation: AAB89160.1.

PIR

G69511.

RefSeq

NP_070920.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RZW	NMR	-	A	1-115	[»]
3ERJ	X-ray	1.80	A/B	1-115	[»]

ModBase

Search...

Genome annotation databases

GeneID

1485324.

GenomeReviews

Gene locus AF_2095 in contig AE000782_GR.

KEGG

afu:AF2095.

NMPDR

fig|224325.1.peg.2081.

TIGR

AF_2095.

Phylogenomic databases

HOGENOM

O28185.

OMA

IQRRNPE.

Enzyme and pathway databases

BioCyc

AFUL224325:AF_2095-MON.

Family and domain databases

HAMAP

MF_00628.
[Tree]

InterPro

IPR002833. Pep_tRNA_hydro_PTH2.
[Graphical view]

Pfam

PF01981. PTH2. 1 hit.
[Graphical view]

ProDom

PD010667. UPF0099. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00283. Pep_tRNA_hydro_PTH2. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PTH_ARCFU

Accession

Primary (citable) accession number: O28185

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)