O28142 (SERB_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine phosphatase
Short name=PSP
Short name=PSPase
EC=3.1.3.3

Alternative name(s):
O-phosphoserine phosphohydrolase

Gene names

Ordered Locus Names:

AF_2138

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.
Sequence similarities	Belongs to the serB family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoserine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phosphoserine phosphatase		PRO_0000156890
Regions
	Domain	47 – 118	72	ACT
	Region	223 – 224	2	Substrate binding By similarity
Sites
	Active site	135	1	Nucleophile By similarity
	Active site	137	1	Proton donor By similarity
	Metal binding	135	1	Magnesium By similarity
	Metal binding	137	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	291	1	Magnesium By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	268	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28142 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2922DBBFD210BBD4	FASTA	344	38,515
	10 20 30 40 50 60 MFVMFIVAEV DDEVCVKREV ERAAGEVGVH VSLTPFQRRE KAEKNLYVVT ILGKDRVGIV 70 80 90 100 110 120 RDITRAFLDF GINIERTSLT AREELISIEF LVDLGQRDAA EVRKRLRREA ERLGLDIVMQ 130 140 150 160 170 180 PYSTFNREKR LIVFDMDSTL VEAEIIDELA KEAGVGDEVS KLTERAMRGE IGFKEALEER 190 200 210 220 230 240 VRLLKGLPVE VLERIYSRIK LTEGAKELVR SLKEAGYKVA VVSGGFSYFT DRLKEELGLD 250 260 270 280 290 300 YAFGNELEIE NGRLTGRIKG RIIDASEKAR IVEEIARKEG ISPENVVAVG DGANDRLMIE 310 320 330 340 RAGLGIAFNA KEVLKDVADG SISKENLVGL ASVLKLPAEF RKKV

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB89113.1.
PIR	B69517.
RefSeq	NP_070963.1. NC_000917.1.
3D structure databases
ProteinModelPortal	O28142.
SMR	O28142. Positions 127-335.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1485367.
GenomeReviews	Gene locus AF_2138 in contig AE000782_GR.
KEGG	afu:AF2138.
NMPDR	fig|224325.1.peg.2124.
TIGR	AF_2138.
Phylogenomic databases
HOGENOM	HBG645339.
OMA	NLYVVTI.
PhylomeDB	O28142.
ProtClustDB	CLSK541965.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_2138-MONOMER.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR006383. HAD-SF_hydro_IB_PSP-like. IPR023190. Pser_Pase_dom_2. IPR004469. SerB. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits. G3DSA:1.10.150.210. Pser_Pase_dom_2. 1 hit.
KO	K01079.
Pfam	PF01842. ACT. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01488. HAD-SF-IB. 1 hit. TIGR00338. SerB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SERB_ARCFU

Accession

Primary (citable) accession number: O28142

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program