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Protein

Catalase-peroxidase

Gene

katG

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Displays also NADH oxidase, INH lyase and isonicotinoyl-NAD synthase activity.

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Kineticsi

  1. KM=32 mM for H2O2 for the catalase reaction (at pH 5.5-6.0)1 Publication
  2. KM=3.8 mM for H2O2 for the catalase reaction (at pH 7.0)1 Publication
  3. KM=95 mM for H2O2 for the peroxidase reaction1 Publication
  4. KM=16 mM for ABTS for the peroxidase reaction1 Publication
  1. Vmax=11760 µmol/min/mg enzyme for H2O2 for the catalase reaction (at pH 5.5-6.0)1 Publication
  2. Vmax=5500 µmol/min/mg enzyme for H2O2 for the catalase reaction (at pH 7.0)1 Publication
  3. Vmax=12 µmol/min/mg enzyme for ABTS for the peroxidase reaction1 Publication

pH dependencei

Optimum pH is 4.5 for the peroxidase reaction.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei83 – 831Transition state stabilizerUniRule annotation
Active sitei87 – 871Proton acceptorUniRule annotation
Metal bindingi249 – 2491Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2280-MONOMER.
BRENDAi1.11.1.21. 414.

Protein family/group databases

PeroxiBasei1858. AfCP01.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Synonyms:perA
Ordered Locus Names:AF_2233
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741Catalase-peroxidasePRO_0000055579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki86 ↔ 208Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-234)UniRule annotation
Cross-linki208 ↔ 234Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-86)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiO28050.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi224325.AF2233.

Structurei

3D structure databases

ProteinModelPortaliO28050.
SMRiO28050. Positions 18-725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04487. Archaea.
COG0376. LUCA.
KOiK03782.
OMAiIAEVYAC.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28050-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRQGGVMVG ARKRWITDWW PNRLNLKILR QNLQNPYGED YDYVEEVENL
60 70 80 90 100
DIDAVIRDLK ELMRSSQDWW PADFGHYGPL FIRLAWHSAG SYRIFDGRGG
110 120 130 140 150
ARDGSIRFPP RINWPDNINL DKAIRLLWPI KKKYGRKLSW ADLIILAGTV
160 170 180 190 200
AMEDMGVKLF GFALGREDIF EPDESPDWGP EEEMLTAKRG EKEELERPFA
210 220 230 240 250
ATEMGLIYVN PEGPGGNPDP LGSAQEIRVA FRRMGMNDEE TVALIAGGHA
260 270 280 290 300
FGKCHGAGPA DYLGPDPSSS PIEMQGLGWK YNYGKGKGSD TFTSGLEVTW
310 320 330 340 350
SPTPTKFGIN YLRILFTYEW ELEKSPAGKN QWVAKDAPEI IPDAHDPNKK
360 370 380 390 400
HRPRMLTADL ALRFDPEFSK IARRFLENPE EFEKAFAIAW YKLTHRDMGP
410 420 430 440 450
KDCYIGKYVP EETFVWQDPL PRRDYELVDE KDVEELKRRI LASGLSLSQL
460 470 480 490 500
VYFAWASAST YRNSDRRGGA NGARIRLKPM SVWEVNHPEE LKKVIAAYEK
510 520 530 540 550
IQQEFNEGAK GSEKRISIAD LIVLGGIAAV EEAARRAGFS VKVPFIPGRV
560 570 580 590 600
DAQQEHVDEE FYRVIEPFAD GFRNYFRYPE RINERDVYTT PEYFLVDKAN
610 620 630 640 650
LLTLTVPEMV VLIGGMRALG ANYSHSDYGV LTERPGVLSN DFFVNLLDMS
660 670 680 690 700
VEWRAADDYR YTFEGYDRKS GELRWRATRV DLILGHHDEL RAVAEVYGCD
710 720 730 740
DAKEKFVKDF AAVCAKVMHL DRFDLWRSNR KLYKEITAGL R
Length:741
Mass (Da):84,854
Last modified:January 1, 1998 - v1
Checksum:iA931DF34F050FC63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89022.1.
PIRiA69529.
RefSeqiWP_010879722.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89022; AAB89022; AF_2233.
GeneIDi24795995.
KEGGiafu:AF_2233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89022.1.
PIRiA69529.
RefSeqiWP_010879722.1. NC_000917.1.

3D structure databases

ProteinModelPortaliO28050.
SMRiO28050. Positions 18-725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2233.

Protein family/group databases

PeroxiBasei1858. AfCP01.

Proteomic databases

PRIDEiO28050.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89022; AAB89022; AF_2233.
GeneIDi24795995.
KEGGiafu:AF_2233.

Phylogenomic databases

eggNOGiarCOG04487. Archaea.
COG0376. LUCA.
KOiK03782.
OMAiIAEVYAC.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2280-MONOMER.
BRENDAi1.11.1.21. 414.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_ARCFU
AccessioniPrimary (citable) accession number: O28050
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.