O28046 (O28046_ARCFU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase HAMAP MF_01405
EC=3.6.1.19 HAMAP MF_01405

Alternative name(s):
Non-standard purine NTP pyrophosphatase HAMAP MF_01405
Nucleoside-triphosphate diphosphatase HAMAP MF_01405
Nucleoside-triphosphate pyrophosphatase HAMAP MF_01405

Gene names

Ordered Locus Names:

AF_2237

Organism

Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomic identifier

224325 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity. HAMAP MF_01405
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family. HAMAP MF_01405 RuleBase RU003781

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_01405
Ligand	Magnesium HAMAP MF_01405 Manganese HAMAP MF_01405 Metal-binding HAMAP MF_01405 Nucleotide-binding HAMAP MF_01405
Molecular function	Hydrolase HAMAP MF_01405 RuleBase RU003781
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	5 – 10	6	Substrate binding By similarity HAMAP MF_01405
	Region	63 – 64	2	Substrate binding By similarity HAMAP MF_01405
Sites
	Metal binding	34	1	Magnesium or manganese By similarity HAMAP MF_01405
	Metal binding	63	1	Magnesium or manganese By similarity HAMAP MF_01405
	Binding site	141	1	Substrate By similarity HAMAP MF_01405
	Binding site	159	1	Substrate By similarity HAMAP MF_01405
	Binding site	165	1	Substrate By similarity HAMAP MF_01405

Sequences

	Sequence		Length	Mass (Da)
	O28046 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E24C38C79AF92113	FASTA	181	20,953
	10 20 30 40 50 60 MYFITSNEGK FREVREMASK YGIEIEWLKM EYIEPQGSSL EEIARLSAEM LAEKVEGEFV 70 80 90 100 110 120 IEDSGLFVEA LKGFPGPYSS YVFKTIGNEG ILKLMEGVEN RKAYFMAVVA YFDGKEVRTF 130 140 150 160 170 180 TGKVEGEISR EMRGTQGFGY DPIFLYGNKT FAEMATEEKN QVSHRRKAFE EFFRWLKENK I

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000782 Genomic DNA. Translation: AAB89015.1.
PIR	E69529.
RefSeq	NP_071062.1. NC_000917.1.
3D structure databases
HSSP	HSSP built from PDB template 1B78 based on UniProtKB Q57679.
ProteinModelPortal	O28046.
SMR	O28046. Positions 1-180.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1485468.
GenomeReviews	Gene locus AF_2237 in contig AE000782_GR.
KEGG	afu:AF2237.
NMPDR	fig|224325.1.peg.2223.
TIGR	AF_2237.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	VEDSGFF.
PhylomeDB	O28046.
ProtClustDB	CLSK2747234.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

O28046_ARCFU

Accession

Primary (citable) accession number: O28046

Entry history

Integrated into UniProtKB/TrEMBL:	January 1, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)