Reviewed, UniProtKB/Swiss-Prot O28034 (PYRC_ARCFU)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	AF_2250

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Dihydroorotase HAMAP MF_00220		PRO_0000147267
Sites
	Metal binding	48	1	Zinc 1 By similarity
	Metal binding	50	1	Zinc 1 By similarity
	Metal binding	172	1	Zinc 2 By similarity
	Metal binding	211	1	Zinc 2 By similarity
	Metal binding	277	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O28034-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7AF4E74CCA4624AF	FASTA	403	45,559
	10 20 30 40 50 60 MIRGKVFYKG EFVEAGIEVE NGRIKRIGKL VEGKEVKGVI LPAGIDVHVH LRDFAEKRKE 70 80 90 100 110 120 TIETGTLSAL HGGICLVVDQ PNTKPPVDDA ETYFRRMGKA EKSVYVDYAL NLALTNSNHG 130 140 150 160 170 180 KIGSIMRKIS ERYFVPAVGE VFIQHDSEDL QIDYETLSSV YKRFEGVVFT IHAEDPAYVA 190 200 210 220 230 240 RGSPNFVFRR REAEVLAVER LVELGKFHFC HISTKDSAKE ILNSNSTYEV TPHHMLLSVE 250 260 270 280 290 300 DYGRLGNLVN VNPPLRERED VEWLFRNFHR IDVLASDHAP HTLEDKEAGA SGFPGVETMY 310 320 330 340 350 360 PLFVNLASKG YISFKTLVEK IASNPARIFG FKGYGEIEVG NYANFAVFDL KKVDEIRAER 370 380 390 400 LHSKCGWTPF EGFEAVFPDK VYLRGKELLE NEMKAGNVLK KRV

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89007.1.
PIR	B69531.
RefSeq	NP_071075.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1485481.
GenomeReviews	Gene locus AF_2250 in contig AE000782_GR.
KEGG	afu:AF2250.
NMPDR	fig|224325.1.peg.2236.
TIGR	AF_2250.
Phylogenomic databases
HOGENOM	O28034.
OMA	YATDHAP.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_2250-MON.
BRENDA	3.5.2.3. 7576.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ARCFU

Accession

Primary (citable) accession number: O28034

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)