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O28032 (ASSY_ARCFU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:AF_2252
OrganismArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) [Reference proteome] [HAMAP]
Taxonomic identifier224325 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000148671

Regions

Nucleotide binding6 – 149ATP By similarity

Sites

Binding site831Citrulline By similarity
Binding site1131ATP; via amide nitrogen By similarity
Binding site1151Aspartate By similarity
Binding site1191Aspartate By similarity
Binding site1191Citrulline By similarity
Binding site1201Aspartate By similarity
Binding site1231Citrulline By similarity
Binding site1691Citrulline By similarity
Binding site1781Citrulline By similarity
Binding site2541Citrulline By similarity
Binding site2661Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
O28032 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 31334D84F696EE8D

FASTA39044,104
        10         20         30         40         50         60 
MKVVLSYSGG LDTTVCIPLL KEKYGFDEVI TVTVDIGQPE ADIKQAEERG KKYADKHYTI 

        70         80         90        100        110        120 
DAKKEFVDSL FMLIKANGNY EGYVLGTALA RPLIAEKVVE VAKKEGAEAV AHGCTGKGND 

       130        140        150        160        170        180 
QLRFENIFRQ HGFKVIAPVR ELNLTREWEI EYARQHGIEV PATKEKPYSI DENLWSRSVE 

       190        200        210        220        230        240 
GGKLEDPSFE PPEDIYEWTA SPEKAPDKPE IVKIDFEKGV PVALNDERMG GFELIKALNE 

       250        260        270        280        290        300 
IGGKHGVGRT DMIEDRVLGL KARENYEHPA ATILITAHRD LENLVLSRRE LKFKKFVEEE 

       310        320        330        340        350        360 
WAELVYYGLV NDPLFDALNA FIDKTQERVT GWVKVKLYKG SAVVVARNSP YALYSEELVS 

       370        380        390 
FDTESIDQRL AEGFAAFHGL QGRLFRRLFQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000782 Genomic DNA. Translation: AAB89005.1.
PIRD69531.
RefSeqNP_071077.1. NC_000917.1.

3D structure databases

ProteinModelPortalO28032.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224325.AF2252.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB89005; AAB89005; AF_2252.
GeneID1485483.
KEGGafu:AF2252.

Phylogenomic databases

eggNOGCOG0137.
KOK01940.
OMARRYDAKY.
ProtClustDBPRK13820.

Enzyme and pathway databases

BioCycAFUL224325:GJBC-2300-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_ARCFU
AccessionPrimary (citable) accession number: O28032
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways