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Protein

Coenzyme F420:L-glutamate ligase

Gene

cofE

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent successive addition of two L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2), with a gamma-linkage between the two glutamates. May be able to add up to four gamma-linked glutamates, since F420-4 is a species that was isolated from A.fulgidus.1 Publication

Catalytic activityi

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.1 Publication
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.1 Publication

Cofactori

Protein has several cofactor binding sites:

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45GTP1
Metal bindingi109Divalent metal cation 11
Binding sitei112GTP1
Metal bindingi150Divalent metal cation 11
Metal bindingi151Divalent metal cation 21
Metal bindingi208Divalent metal cation 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 14GTP4
Nucleotide bindingi40 – 41GTP2
Nucleotide bindingi206 – 213GTP8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

GTP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BRENDAi6.3.2.31. 11304.
6.3.2.34. 11304.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420:L-glutamate ligase (EC:6.3.2.31, EC:6.3.2.34)
Alternative name(s):
Coenzyme F420-0:L-glutamate ligase
Coenzyme F420-1:gamma-L-glutamate ligase
F420:glutamyl ligase
Gene namesi
Name:cofE
Ordered Locus Names:AF_2256
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457851 – 249Coenzyme F420:L-glutamate ligaseAdd BLAST249

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224325.AF2256.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi20 – 25Combined sources6
Beta strandi35 – 39Combined sources5
Helixi40 – 46Combined sources7
Beta strandi50 – 52Combined sources3
Helixi53 – 55Combined sources3
Helixi60 – 69Combined sources10
Helixi73 – 81Combined sources9
Beta strandi83 – 88Combined sources6
Beta strandi90 – 92Combined sources3
Beta strandi94 – 97Combined sources4
Beta strandi102 – 104Combined sources3
Helixi105 – 107Combined sources3
Beta strandi111 – 113Combined sources3
Beta strandi117 – 119Combined sources3
Helixi125 – 140Combined sources16
Beta strandi145 – 154Combined sources10
Beta strandi157 – 170Combined sources14
Beta strandi172 – 175Combined sources4
Beta strandi191 – 193Combined sources3
Helixi194 – 206Combined sources13
Beta strandi208 – 211Combined sources4
Beta strandi215 – 220Combined sources6
Helixi229 – 231Combined sources3
Turni236 – 238Combined sources3
Helixi240 – 248Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G9IX-ray2.50A/B1-249[»]
2PHNX-ray1.35A/B1-249[»]
ProteinModelPortaliO28028.
SMRiO28028.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28028.

Family & Domainsi

Sequence similaritiesi

Belongs to the CofE family.Curated

Phylogenomic databases

eggNOGiarCOG02714. Archaea.
COG1478. LUCA.
KOiK12234.
OMAiLTETRFG.

Family and domain databases

HAMAPiMF_01258. F420_ligase_CofE. 1 hit.
InterProiIPR008225. F420-0_g-glutamyl_ligase.
IPR002847. F420-0_gamma-glut_ligase-dom.
IPR023659. F420_ligase_CofE_arc.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.

Sequencei

Sequence statusi: Complete.

O28028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVEVFPVEG LPLIKEGDDL AELISSRVRF EDGDVLVVCS TVISKAEGRI
60 70 80 90 100
RRLEEFNPSE RAKEIAARIG KPAEFVQAVL EESEEVLLDF PFLLVKAKFG
110 120 130 140 150
NVCVNAGIDA SNVEEGSLLL PPLDPDGSAE KLRRRILELT GKRVGVIITD
160 170 180 190 200
TNGRCFRRGV VGFAIGISGV KAMKDWIGRK DLYGRELEVT VECVADEIAA
210 220 230 240
FANLLMGEGG DGIPAVVVRG LNVAGEGSME EIYRSEEEDV IRRCLKRCL
Length:249
Mass (Da):27,261
Last modified:January 1, 1998 - v1
Checksum:iEC64A6D40FDD8B97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89001.1.
PIRiH69531.
RefSeqiWP_010879745.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB89001; AAB89001; AF_2256.
GeneIDi24796019.
KEGGiafu:AF_2256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB89001.1.
PIRiH69531.
RefSeqiWP_010879745.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G9IX-ray2.50A/B1-249[»]
2PHNX-ray1.35A/B1-249[»]
ProteinModelPortaliO28028.
SMRiO28028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB89001; AAB89001; AF_2256.
GeneIDi24796019.
KEGGiafu:AF_2256.

Phylogenomic databases

eggNOGiarCOG02714. Archaea.
COG1478. LUCA.
KOiK12234.
OMAiLTETRFG.

Enzyme and pathway databases

UniPathwayiUPA00071.
BRENDAi6.3.2.31. 11304.
6.3.2.34. 11304.

Miscellaneous databases

EvolutionaryTraceiO28028.

Family and domain databases

HAMAPiMF_01258. F420_ligase_CofE. 1 hit.
InterProiIPR008225. F420-0_g-glutamyl_ligase.
IPR002847. F420-0_gamma-glut_ligase-dom.
IPR023659. F420_ligase_CofE_arc.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOFE_ARCFU
AccessioniPrimary (citable) accession number: O28028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.