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Protein

tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS

Gene

tiaS

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.1 Publication

Catalytic activityi

ATP + agmatine + (tRNA(Ile)2)-cytidine(34) + H2O = (tRNA(Ile)2)-2-agmatinylcytidine(34) + AMP + 2 phosphate.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi268 – 32962OBAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ligase activity, forming carbon-nitrogen bonds Source: UniProtKB
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • tRNA wobble cytosine modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2308-MONOMER.
BRENDAi6.3.4.22. 414.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS (EC:6.3.4.22)
Short name:
tRNA(Ile2)-agm2C synthetase
Alternative name(s):
tRNA(Ile2) agmatidine synthetase
Gene namesi
Name:tiaS
Ordered Locus Names:AF_2259
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi140 – 1401R → A: Loss of activity. 1 Publication
Mutagenesisi141 – 1411G → A: Loss of activity. 1 Publication
Mutagenesisi163 – 1631Y → A: Decrease in activity. 1 Publication
Mutagenesisi164 – 1641R → A: Decrease in activity. 1 Publication
Mutagenesisi217 – 2171R → A: Decrease in activity. 1 Publication
Mutagenesisi218 – 2181G → A: Decrease in activity. 1 Publication
Mutagenesisi248 – 2481T → A: Decrease in activity. 1 Publication
Mutagenesisi249 – 2491D → A: Decrease in activity. 1 Publication
Mutagenesisi352 – 3521C → A: Almost loss of activity. 1 Publication
Mutagenesisi355 – 3551C → A: Almost loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420tRNA(Ile2) 2-agmatinylcytidine synthetase TiaSPRO_0000407288Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224325.AF2259.

Structurei

Secondary structure

420
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 77Combined sources
Beta strandi13 – 153Combined sources
Helixi20 – 3213Combined sources
Beta strandi35 – 4410Combined sources
Beta strandi53 – 553Combined sources
Beta strandi57 – 6610Combined sources
Helixi68 – 7811Combined sources
Beta strandi87 – 893Combined sources
Beta strandi94 – 985Combined sources
Helixi99 – 1024Combined sources
Turni103 – 1053Combined sources
Helixi106 – 1149Combined sources
Helixi119 – 12810Combined sources
Beta strandi133 – 1353Combined sources
Helixi141 – 15010Combined sources
Beta strandi157 – 1637Combined sources
Helixi166 – 1683Combined sources
Beta strandi169 – 1713Combined sources
Helixi177 – 18711Combined sources
Beta strandi190 – 1967Combined sources
Turni197 – 2004Combined sources
Beta strandi203 – 2053Combined sources
Beta strandi208 – 21912Combined sources
Helixi221 – 23010Combined sources
Beta strandi239 – 2446Combined sources
Beta strandi252 – 2543Combined sources
Helixi255 – 2573Combined sources
Beta strandi266 – 28015Combined sources
Turni281 – 2833Combined sources
Beta strandi284 – 2918Combined sources
Beta strandi294 – 3007Combined sources
Helixi302 – 3065Combined sources
Helixi307 – 3115Combined sources
Beta strandi319 – 3279Combined sources
Beta strandi330 – 34011Combined sources
Beta strandi344 – 3463Combined sources
Turni353 – 3553Combined sources
Beta strandi359 – 3613Combined sources
Beta strandi363 – 3653Combined sources
Beta strandi368 – 3703Combined sources
Turni371 – 3744Combined sources
Beta strandi375 – 3773Combined sources
Beta strandi383 – 3853Combined sources
Beta strandi391 – 3955Combined sources
Helixi398 – 4003Combined sources
Beta strandi403 – 4053Combined sources
Helixi408 – 4103Combined sources
Beta strandi414 – 4163Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AMTX-ray2.90A1-420[»]
3AMUX-ray3.10A1-420[»]
3AU7X-ray2.60A1-343[»]
A386-420[»]
4RVZX-ray2.90Z1-420[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO28025.

Family & Domainsi

Sequence similaritiesi

Belongs to the TiaS family.Curated
Contains 1 OB DNA-binding domain.Curated

Phylogenomic databases

eggNOGiarCOG01115. Archaea.
COG1571. LUCA.
KOiK06932.
OMAiGMCTTYL.

Family and domain databases

HAMAPiMF_01892. tRNA_Ile2_agm2C_synt. 1 hit.
InterProiIPR013696. DUF1743.
IPR004365. NA-bd_OB_tRNA.
IPR024913. tRNA_Ile2__agm2C_synt.
[Graphical view]
PfamiPF08489. DUF1743. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O28025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVWVGIDDT DSSRGMCTTY LAVLAMERVE RELGKVIGFP RLIRLNPTIP
60 70 80 90 100
YKTRGNGAVS FLVEVDDVGE LVDVVNEVII EHAMLDDEKT NPGAVFVDEE
110 120 130 140 150
LAVKLKPFAD KAIKDVLQID EALFVIGKYF IPHLRHKKGR GLIGALAAVG
160 170 180 190 200
AELEDFTLEL IAYRYPERFG TEREYDEESF FDMDYELYPQ TFDNVDWCND
210 220 230 240 250
VVVCIPNTPC PVLYGIRGES VEALYKAMES VKTEPVDRRM IFVTNHATDM
260 270 280 290 300
HLIGEEEVHR LENYRSYRLR GRVTLEPYDI EGGHVFFEID TKFGSVKCAA
310 320 330 340 350
FEPTKQFRNV IRLLRKGDVV EVYGSMKKDT INLEKIQIVE LAEIWVEKNP
360 370 380 390 400
ICPSCGRRME SAGRGQGFRC KKCRTKADEK LREKVERELQ PGFYEVPPSA
410 420
RRHLSKPLIR MNVEGRHIFR
Length:420
Mass (Da):48,222
Last modified:January 1, 1998 - v1
Checksum:i846D3F445EEC4885
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB88990.1.
PIRiC69532.
RefSeqiWP_010879748.1. NC_000917.1.

Genome annotation databases

EnsemblBacteriaiAAB88990; AAB88990; AF_2259.
GeneIDi24796023.
KEGGiafu:AF_2259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB88990.1.
PIRiC69532.
RefSeqiWP_010879748.1. NC_000917.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AMTX-ray2.90A1-420[»]
3AMUX-ray3.10A1-420[»]
3AU7X-ray2.60A1-343[»]
A386-420[»]
4RVZX-ray2.90Z1-420[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224325.AF2259.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB88990; AAB88990; AF_2259.
GeneIDi24796023.
KEGGiafu:AF_2259.

Phylogenomic databases

eggNOGiarCOG01115. Archaea.
COG1571. LUCA.
KOiK06932.
OMAiGMCTTYL.

Enzyme and pathway databases

BioCyciAFUL224325:GJBC-2308-MONOMER.
BRENDAi6.3.4.22. 414.

Miscellaneous databases

EvolutionaryTraceiO28025.

Family and domain databases

HAMAPiMF_01892. tRNA_Ile2_agm2C_synt. 1 hit.
InterProiIPR013696. DUF1743.
IPR004365. NA-bd_OB_tRNA.
IPR024913. tRNA_Ile2__agm2C_synt.
[Graphical view]
PfamiPF08489. DUF1743. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIAS_ARCFU
AccessioniPrimary (citable) accession number: O28025
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.