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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPBy similarity1
Active sitei306Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi281 – 285ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:G1G16-1869-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
Name:rtcA
Ordered Locus Names:MTH_1915
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564291 – 337RNA 3'-terminal phosphate cyclaseAdd BLAST337

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH1915

Structurei

3D structure databases

ProteinModelPortaliO27937
SMRiO27937
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG04125 Archaea
COG0430 LUCA
KOiK01974
OMAiANRPKPG
OrthoDBiPOG093Z05RD

Family and domain databases

Gene3Di3.30.360.201 hit
3.65.10.202 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF55205 SSF55205, 2 hits
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

O27937-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVIDGSEGE GGGAVVRVST ALAAVTSRSV RVYNIRARRS RSGLSHQHLT
60 70 80 90 100
AVRAVARISN GTLRGDELGS MELEFSPGRV TGGTFNFDVK TAGSTGLVLQ
110 120 130 140 150
AIMVAAAASE GEIDVTVSGG TDVLWAPTCD YLREVTIPVL EMMGYSARIE
160 170 180 190 200
IIRRGYYPEG GGRVHAIIEP SELRPITLEE SEIHAVRGIS HSRNLPVHVA
210 220 230 240 250
ERQAESAMKI LRGAGLDVDI MVEDASGPVG RGSGITLWAE GNTRLGAVSL
260 270 280 290 300
GKPGKRAEKV GSEAARELLG FIESGSPLDR YMGDQIIPYM ALTGDSRVRT
310 320 330
CELTLHAETN IILSEKITGR RFRVEGERGG PATIEVL
Length:337
Mass (Da):35,932
Last modified:January 1, 1998 - v1
Checksum:i0D893466978743FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB86375.1
PIRiG69122
RefSeqiWP_010877511.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaiAAB86375; AAB86375; MTH_1915
GeneIDi1471000
KEGGimth:MTH_1915
PATRICifig|187420.15.peg.1871

Similar proteinsi

Entry informationi

Entry nameiRTCA_METTH
AccessioniPrimary (citable) accession number: O27937
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome