Reviewed, UniProtKB/Swiss-Prot O27907 (HDRB_METTH)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase subunit B
    EC=1.8.98.1

Gene names

Name:	hdrB
Ordered Locus Names:	MTH_1879

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrB may have a function in energy transduction By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Pathway	Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase is composed of three subunits; hdrA, hdrB and hdrC. It forms a complex with the F420-non-reducing hydrogenase (Mvh), which provides the reducing equivalents to the heterodisulfide reductase By similarity.
Sequence similarities	Belongs to the hdrB family.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cofactor metabolic process Inferred from electronic annotation. Source: InterPro methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	CoB--CoM heterodisulfide reductase subunit B		PRO_0000150069

Sequences

	Sequence		Length	Mass (Da)
	O27907-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1BE40A77DE7DDFDC	FASTA	302	33,513
	10 20 30 40 50 60 MEIAYFLGCI MNNRYPGIEK ATRVLFDKLG IELKDMEGAF CCPAPGVFGS FDKTTWAAIA 70 80 90 100 110 120 ARNITIAEEM GSDVMTECNG CFGSLFEANH LLKEDEEMRA KINEILKEAG REYKGEINVR 130 140 150 160 170 180 HLAEILYNDV GLDKLSEVVE KPLNLNVAVH YGCHFLKPSE EINIDNPERP TILDELVEVT 190 200 210 220 230 240 GAKSVDYKDK MMCCGAGGGV RSRDLDVALD FTMEKLRNMK EAGVDAIVNV CPFCHLQFDV 250 260 270 280 290 300 GQMEIKDKFG EEFDIPVLHL AQLLGLAMGL PKEDLVVDAH QVCVDECLEK LEELDRLAPG SG

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB86345.1.
PIR	D69118.
RefSeq	NP_276985.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O27907.
Genome annotation databases
GeneID	1470964.
GenomeReviews	Gene locus MTH_1879 in contig AE000666_GR.
KEGG	mth:MTH1879.
NMPDR	fig|187420.1.peg.1840.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04955.
HOGENOM	HBG513215.
OMA	DKMMCCG.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1879-MONOMER.
BRENDA	1.8.98.1. 270.
Family and domain databases
InterPro	IPR017678. CoB/CoM_hetero-S_Rdtase_bsu. IPR004017. Cys_rich_dom. [Graphical view]
Pfam	PF02754. CCG. 2 hits. [Graphical view]
TIGRFAMs	TIGR03288. CoB_CoM_SS_B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDRB_METTH

Accession

Primary (citable) accession number: O27907

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)