O27906 (HDRC_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CoB--CoM heterodisulfide reductase iron-sulfur subunit C
EC=1.8.98.1

Gene names

Name:	hdrC
Ordered Locus Names:	MTH_1878

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrC may carry electrons from hydrogenase to hdrA By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Pathway	Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase is composed of three subunits; hdrA, hdrB and hdrC. It forms a complex with the F420-non-reducing hydrogenase (Mvh), which provides the reducing equivalents to the heterodisulfide reductase By similarity.
Sequence similarities	Belongs to the hdrC family. Contains 2 4Fe-4S ferredoxin-type domains.
Sequence caution	The sequence AAB86344.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cofactor metabolic process Inferred from electronic annotation. Source: InterPro methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	CoB--CoM heterodisulfide reductase iron-sulfur subunit C		PRO_0000150077
Regions
	Domain	25 – 55	31	4Fe-4S ferredoxin-type 1
	Domain	68 – 99	32	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	35	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	38	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	41	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	45	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	79	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	82	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	85	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	89	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O27906 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: 7DD936AFBDF9D499	FASTA	185	20,490
	10 20 30 40 50 60 MTLLQREENI IRKGNIDKEF SEKIKAAGGD SLEYCFQCGT CTGSCPSGRR TPYRVRQIIR 70 80 90 100 110 120 KANVGLKDEI ISDPALWMCT TCYSCQERCP RKVKIVDVVK LARNEAAKAG FMAPAHKAVG 130 140 150 160 170 180 SFVIKTGHGV PINDATMELR KAVGLGELPP TTHQFPEALE EVQKIIKATG FDQLIGYNWE TGELE

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB86344.1. Different initiation.
PIR	C69118.
RefSeq	NP_276984.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27906.
ModBase	Search...
Protein-protein interaction databases
IntAct	O27906. 1 interaction.
STRING	O27906.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470963.
GenomeReviews	Gene locus MTH_1878 in contig AE000666_GR.
KEGG	mth:MTH1878.
Phylogenomic databases
eggNOG	arNOG07412.
HOGENOM	HBG542047.
OMA	KTGHAVP.
ProtClustDB	CLSK798298.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1878-MONOMER.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017680. CoB/CoM_hetero-S_Rdtase_csu. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
KO	K03390.
SUPFAM	SSF46548. Helical_ferredxn. 1 hit.
TIGRFAMs	TIGR03290. CoB_CoM_SS_C. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HDRC_METTH

Accession

Primary (citable) accession number: O27906

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	November 16, 2011
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program