O27880 (IORA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Indolepyruvate oxidoreductase subunit iorA
Short name=IOR
EC=1.2.7.8

Alternative name(s):
Indolepyruvate ferredoxin oxidoreductase subunit alpha

Gene names

Name:	iorA
Ordered Locus Names:	MTH_1852

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates By similarity.
Catalytic activity	(Indol-3-yl)pyruvate + CoA + 2 oxidized ferredoxin = S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+.
Cofactor	Binds 1 4Fe-4S cluster.
Subunit structure	Heterodimer of the iorA and iorB subunits.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro indolepyruvate ferredoxin oxidoreductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Indolepyruvate oxidoreductase subunit iorA		PRO_0000099926
Regions
	Domain	558 – 587	30	4Fe-4S ferredoxin-type 1
	Domain	588 – 617	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	597	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	600	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	603	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	607	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O27880 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B2D222FF7983F203	FASTA	618	66,871
	10 20 30 40 50 60 MELEDILNAR EGDKLFLLGN EATVRAAIES GVGVASTYPG TPSSEIGNVL SGIAKRAGMY 70 80 90 100 110 120 FEFSVNEKVA LEVAAAAAAS GVRSFTFMKH VGLNVASDSF MSTAYTGVRA GMVVLTADDP 130 140 150 160 170 180 SMFSSQNEQD NRHYARLACL PLLEPSDPQE VLEFMNHAFE LSEDYGLPVL LRTTTRVSHM 190 200 210 220 230 240 RGVVEVGSRM REPSEGFFRK DPERFVPVPA TARVMHRKLV DKMKELRIRA DESELNRVFN 250 260 270 280 290 300 GGSDSELGVV ASGGAFNYVY DALESLGLEL PVLKLGFTYP FPAGLVEEFL SGLKRVLVVE 310 320 330 340 350 360 EVDPIMEREV LAVAGSARLD LDVHGKLDGT LPEIYEYNED ILRKAISGLT GAPSVERECD 370 380 390 400 410 420 VPDIPERPPS LCPGCPHRAV YYAVRRAADE LELSGDEIIF PTDIGCYTLG IEPPYSAADY 430 440 450 460 470 480 LLSMGSSIGT SCGFSAATTQ RIVSFIGDST FFHAGIPPLI NAVHNKQRFV LVVLDNRTTA 490 500 510 520 530 540 MTGGQPHPGL PVDGMGDEAP EISIEEIVRA SGVEFVETVN PMNIKRTSET VKRALEHESV 550 560 570 580 590 600 AVVISKYPCM LSSGAVRGRP MAVDGEKCDL CLECIRDLAC PAMVTREGEV FIDPLKCRGC 610 SVCLQICPAG AIKPEGKG

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB86318.1.
PIR	G69114.
RefSeq	NP_276958.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27880.
ModBase	Search...
Protein-protein interaction databases
STRING	O27880.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470937.
GenomeReviews	Gene locus MTH_1852 in contig AE000666_GR.
KEGG	mth:MTH1852.
NMPDR	fig|187420.1.peg.1813.
Phylogenomic databases
eggNOG	arNOG04233.
HOGENOM	HBG733885.
OMA	VADDPSM.
ProtClustDB	CLSK798288.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1852-MONOMER.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017721. Indolepyruvate_Fd_OxRdtase_asu. IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. [Graphical view]
KO	K00179.
Pfam	PF00037. Fer4. 1 hit. PF01855. POR_N. 1 hit. PF02775. TPP_enzyme_C. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR03336. IOR_alpha. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IORA_METTH

Accession

Primary (citable) accession number: O27880

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program