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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331TyrosineUniRule annotation
Binding sitei155 – 1551TyrosineUniRule annotation
Binding sitei159 – 1591TyrosineUniRule annotation
Binding sitei162 – 1621TyrosineUniRule annotation
Binding sitei177 – 1771TyrosineUniRule annotation
Binding sitei215 – 2151ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1771-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)
Alternative name(s):
Tyrosyl-tRNA synthetaseUniRule annotation
Short name:
TyrRSUniRule annotation
Gene namesi
Name:tyrSUniRule annotation
Ordered Locus Names:MTH_1767
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Tyrosine--tRNA ligasePRO_0000055670Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1767.

Structurei

3D structure databases

ProteinModelPortaliO27795.
SMRiO27795. Positions 4-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 469"HIGH" region
Motifi212 – 2165"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
KOiK01866.
OMAiFEYHVFP.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02008. Tyr_tRNA_synth_type3.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023684. Tyr-tRNA-ligase_3.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDSLKTITR DVLEVITPEE LMDVLKKDEP VVYTGYEPSG RIHLGHALTV
60 70 80 90 100
RKLRELQDAG FRVKILLADY HAYLNGKGSM ERIRELADYN QKCFLALGLS
110 120 130 140 150
PERTEFILGS SFQTEPEYTD LVYRLALITT LLRAKRSMAQ ITRESRDHKV
160 170 180 190 200
AEVIYPLMQV IDMVYLGVDV ALGGMEQRKI HMLARENLPR LGFDAPVCIH
210 220 230 240 250
TPLLHGTDGS EKMSSSKGNF IAVDDSPDEI REKVKKSYCP MGETEGNPII
260 270 280 290 300
EIVKYFIMPE YGMMTIRRPE KFGGDLELEL DELLEMYSSS ELHPLDLKNA
310
VSDYLVDMLE PVREYMEGV
Length:319
Mass (Da):36,343
Last modified:January 1, 1998 - v1
Checksum:i6B97EE63C64B590D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB86233.1.
PIRiH69102.
RefSeqiWP_010877369.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB86233; AAB86233; MTH_1767.
GeneIDi1470852.
KEGGimth:MTH_1767.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB86233.1.
PIRiH69102.
RefSeqiWP_010877369.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27795.
SMRiO27795. Positions 4-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1767.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB86233; AAB86233; MTH_1767.
GeneIDi1470852.
KEGGimth:MTH_1767.

Phylogenomic databases

eggNOGiarCOG01886. Archaea.
COG0162. LUCA.
KOiK01866.
OMAiFEYHVFP.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1771-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_02008. Tyr_tRNA_synth_type3.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002307. Tyr-tRNA-ligase.
IPR023684. Tyr-tRNA-ligase_3.
IPR023617. Tyr-tRNA-ligase_arc/euk-type.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PIRSFiPIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTSiPR01040. TRNASYNTHTYR.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiSYY_METTH
AccessioniPrimary (citable) accession number: O27795
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.