ID   MER_METTH               Reviewed;         321 AA.
AC   O27784; Q50540;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 62.
DE   RecName: Full=5,10-methylenetetrahydromethanopterin reductase;
DE            EC=1.5.99.11;
DE   AltName: Full=Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase;
DE   AltName: Full=Methylene-H(4)MPT reductase;
GN   Name=mer; OrderedLocusNames=MTH_1752;
OS   Methanobacterium thermoautotrophicum.
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Delta H;
RX   MEDLINE=96099310; PubMed=8522533;
RA   Noelling J., Pihl T.D., Reeve J.N.;
RT   "Cloning, sequencing, and growth phase-dependent transcription of the
RT   coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin
RT   reductase-encoding genes from Methanobacterium thermoautotrophicum
RT   delta H and Methanopyrus kandleri.";
RL   J. Bacteriol. 177:7238-7244(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Delta H;
RX   MEDLINE=98037514; PubMed=9371463;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA   Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA   Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA   Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA   McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA   Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum
RT   deltaH: functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
RN   [3]
RP   CHARACTERIZATION.
RC   STRAIN=Delta H;
RX   MEDLINE=90130421; PubMed=2298726;
RA   te Broemmelstroet B.W., Hensgens C.M.H., Keltjens J.T.,
RA   van der Drift C., Vogels G.D.;
RT   "Purification and properties of 5,10-methylenetetrahydromethanopterin
RT   reductase, a coenzyme F420-dependent enzyme, from Methanobacterium
RT   thermoautotrophicum strain delta H.";
RL   J. Biol. Chem. 265:1852-1857(1990).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methylene-H(4)MPT
CC       to methyl-H(4)MPT.
CC   -!- CATALYTIC ACTIVITY: 5-methyltetrahydromethanopterin + coenzyme
CC       F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme
CC       F420.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7;
CC       Temperature dependence:
CC         Optimum temperature is 40 degrees Celsius;
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from carbone
CC       dioxide; methyl-CoM from 5,10-methylene-H(4)MPT: step 1/2.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- MISCELLANEOUS: Oxygen-stable.
CC   -!- SIMILARITY: Belongs to the mer family.
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DR   EMBL; U31568; AAA92087.1; -; Genomic_DNA.
DR   EMBL; AE000666; AAB86222.1; -; Genomic_DNA.
DR   PIR; D69101; D69101.
DR   RefSeq; NP_276862.1; -.
DR   HSSP; Q50744; 1F07.
DR   SMR; O27784; 1-321.
DR   GeneID; 1470837; -.
DR   GenomeReviews; AE000666_GR; MTH_1752.
DR   KEGG; mth:MTH1752; -.
DR   NMPDR; fig|187420.1.peg.1717; -.
DR   HOGENOM; O27784; -.
DR   OMA; O27784; KIPIYMG.
DR   BioCyc; MetaCyc:MERMAUTO-MON; -.
DR   BioCyc; MTHE187420:MTH1752-MON; -.
DR   BRENDA; 1.5.99.11; 270.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetr...; IEA:HAMAP.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01091; -; 1.
DR   InterPro; IPR011251; Luciferase-like.
DR   InterPro; IPR016048; Luciferase-like_sub.
DR   InterPro; IPR019946; MeH4methanopterin_reductase.
DR   Gene3D; G3DSA:3.20.20.30; Luciferase_like; 1.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   TIGRFAMs; TIGR03555; F420_mer; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; Methanogenesis; One-carbon metabolism;
KW   Oxidoreductase.
FT   CHAIN         1    321       5,10-methylenetetrahydromethanopterin
FT                                reductase.
FT                                /FTId=PRO_0000084810.
FT   CONFLICT    313    313       L -> H (in Ref. 1; AAA92087).
SQ   SEQUENCE   321 AA;  33479 MW;  F1E7B93500E3C852 CRC64;
     MKFGIEFVPN EPIEKIVKLV KLAEDVGFEY AWITDHYNNK NVYETLALIA EGTETIKLGP
     GVTNPYVRSP AITASAIATL DELSNGRATL GIGPGDKATF DALGIEWVKP VSTIRDAIAM
     MRTLLAGEKT ESGAQLMGVK AVQEKIPIYM GAQGPMMLKT AGEISDGALI NASNPKDFEA
     AVPLIKEGAE SAGKSLSDID VAAYTCCSID EDSAAAANAA KIVVAFIAAG SPPPVFERHG
     LPADTGAKFG ELLGKGDFGG AIGAVDDALM EAFSVVGTPD EFIPKIEALG EMGVTQYVAG
     SPIGPDKEKS IKLLGEVIAS F
//