O27784 (MER_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydromethanopterin reductase
EC=1.5.99.11

Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	MTH_1752

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT. HAMAP MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP MF_01091
Subcellular location	Cytoplasm Probable HAMAP MF_01091.
Miscellaneous	Oxygen-stable. HAMAP MF_01091
Sequence similarities	Belongs to the mer family.
Biophysicochemical properties	pH dependence: Optimum pH is 7. HAMAP MF_01091 Temperature dependence: Optimum temperature is 40 degrees Celsius.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091		PRO_0000084810
Experimental info
	Sequence conflict	313	1	L → H in AAA92087. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O27784 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: F1E7B93500E3C852	FASTA	321	33,479
	10 20 30 40 50 60 MKFGIEFVPN EPIEKIVKLV KLAEDVGFEY AWITDHYNNK NVYETLALIA EGTETIKLGP 70 80 90 100 110 120 GVTNPYVRSP AITASAIATL DELSNGRATL GIGPGDKATF DALGIEWVKP VSTIRDAIAM 130 140 150 160 170 180 MRTLLAGEKT ESGAQLMGVK AVQEKIPIYM GAQGPMMLKT AGEISDGALI NASNPKDFEA 190 200 210 220 230 240 AVPLIKEGAE SAGKSLSDID VAAYTCCSID EDSAAAANAA KIVVAFIAAG SPPPVFERHG 250 260 270 280 290 300 LPADTGAKFG ELLGKGDFGG AIGAVDDALM EAFSVVGTPD EFIPKIEALG EMGVTQYVAG 310 320 SPIGPDKEKS IKLLGEVIAS F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri." Noelling J., Pihl T.D., Reeve J.N. J. Bacteriol. 177:7238-7244(1995) [PubMed: 8522533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Delta H.
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.
[3]	"Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta H." te Broemmelstroet B.W., Hensgens C.M.H., Keltjens J.T., van der Drift C., Vogels G.D. J. Biol. Chem. 265:1852-1857(1990) [PubMed: 2298726] [Abstract] Cited for: CHARACTERIZATION. Strain: Delta H.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31568 Genomic DNA. Translation: AAA92087.1. AE000666 Genomic DNA. Translation: AAB86222.1.
PIR	D69101.
RefSeq	NP_276862.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27784.
SMR	O27784. Positions 1-321.
ModBase	Search...
Protein-protein interaction databases
STRING	O27784.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470837.
GenomeReviews	Gene locus MTH_1752 in contig AE000666_GR.
KEGG	mth:MTH1752.
NMPDR	fig|187420.1.peg.1717.
Phylogenomic databases
eggNOG	arNOG04928.
HOGENOM	HBG497169.
OMA	MGVTQIV.
ProtClustDB	PRK02271.
Enzyme and pathway databases
BioCyc	MetaCyc:MERMAUTO-MONOMER. MTHE187420:MTH1752-MONOMER.
Family and domain databases
HAMAP	MF_01091. F420_mer. [Tree]
InterPro	IPR011251. Luciferase-like_dom. IPR019946. MeH4methanopterin_reductase. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
KO	K00320.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
SUPFAM	SSF51679. Luciferase_like. 1 hit.
TIGRFAMs	TIGR03555. F420_mer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MER_METTH

Accession

Primary (citable) accession number: O27784
Secondary accession number(s): Q50540

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program