O27743 (ACDA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA decarbonylase/synthase complex subunit alpha
Short name=ACDS complex subunit alpha
EC=1.2.99.2

Alternative name(s):
ACDS complex carbon monoxide dehydrogenase
Short name=ACDS CODH

Gene names

Name:	cdhA
Ordered Locus Names:	MTH_1708

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	780 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO2 By similarity. HAMAP MF_01137
Catalytic activity	CO + H2O + A = CO2 + AH2. HAMAP MF_01137
Cofactor	Binds 7 4Fe-4S clusters per heterotetramer Potential. Binds 2 nickel-iron-sulfur clusters per heterotetramer Potential.
Subunit structure	Heterotetramer of two alpha and two epsilon chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha2epsilon2)(4)-beta(8)-(gamma1delta1)8 Potential.
Domain	Cluster B is an all-cysteinyl-liganded 4Fe4S cluster; cluster C is a mixed Ni-Fe-S cluster which appears to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe4S cluster that bridges the two subunits of the CODH dimer. May contain two additional 4Fe-4S clusters, dubbed E and F, that might reroute electron transfer along different paths. HAMAP MF_01137
Sequence similarities	Belongs to the Ni-containing carbon monoxide dehydrogenase family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	acetyl-CoA metabolic process Inferred from electronic annotation. Source: InterPro generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-monoxide dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 780	780	Acetyl-CoA decarbonylase/synthase complex subunit alpha HAMAP MF_01137		PRO_0000155085
Regions
	Domain	399 – 429	31	4Fe-4S ferredoxin-type 1
	Domain	440 – 469	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	73	1	Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	76	1	Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	77	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	79	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	84	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	93	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	250	1	Nickel-iron-sulfur By similarity
	Metal binding	278	1	Nickel-iron-sulfur By similarity
	Metal binding	317	1	Nickel-iron-sulfur By similarity
	Metal binding	409	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	412	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	415	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	419	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	449	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	452	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	455	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	459	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	517	1	Nickel-iron-sulfur By similarity
	Metal binding	546	1	Nickel-iron-sulfur By similarity
	Metal binding	581	1	Nickel-iron-sulfur By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27743 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 98AB06D10C300685	FASTA	780	86,068
	10 20 30 40 50 60 MIDVAPESKK AKDLKGDFWD AKNIQISIGE IITEEKPPEE EVKGPKPRPH VTDLRSWDMK 70 80 90 100 110 120 LLERYEPFYA PFCDMCCLCT YGKCELLGKK GACGIDAATQ QARTVLLACL IGTAAHAGHA 130 140 150 160 170 180 RHLVDHLIER LGEDYKIDLG SNVDIEAPIT RTVMGKRPAT LGDLREVMDY AEEQMSHLLS 190 200 210 220 230 240 ACHTGQEGDS KDFESKAFHA GLMDDLTREV ADLAQIVALD LPKGDEDAPL VELGFGTIDT 250 260 270 280 290 300 EKPVVLCIGH NVLPGADIVD YLDENEMEDQ VEVCGICCAA IDVTRYNEAA KVVGPLSKQL 310 320 330 340 350 360 RFIRSGVADV IVVDEQCVRT DVLEEALKNR SAVIATTDKM CLGLPDMTDE DPDKIVNDLI 370 380 390 400 410 420 NGNIEGALIL DPEKVGEVAV KTAMKLAPIR KSLKKLPDID EIIELASECT DCGWCQRVCP 430 440 450 460 470 480 NSLPVMDAVK KAADGDLSKL EEMAIEELCY TCGRCEQECE RNIPIVSMVT KAGERRVKDE 490 500 510 520 530 540 KYRIRAGRGP AQDVEIRRVG APIVLGDIPG VVAFVGCSNY PEGGKDVALM AKEFLERNYI 550 560 570 580 590 600 VVTTGCGAMS IGEYRDEDGQ TLYEKYGGQF DAKGLVNMGS CVSNAHVSGA AIKIANIFAQ 610 620 630 640 650 660 KPLEGNFEEI ADYILNRVGA CGVAWGAYSQ KAAAIATGVN RWGIPVVLGP HGSKYRRLFL 670 680 690 700 710 720 GRADDEEKWK LKDLRTGEVI DGEPAPEHLL YAAENREEAT VMIAKLCIRP TDTPKGRQMK 730 740 750 760 770 780 LSNYIDLHRK YLGTIPDDID RFIRTEKDIP IVYKRDVMKI LEEKNWKPRE LPKEPSLLER

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB86180.1.
PIR	E69095.
RefSeq	NP_276820.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27743.
ModBase	Search...
Protein-protein interaction databases
IntAct	O27743. 2 interactions.
STRING	O27743.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470793.
GenomeReviews	Gene locus MTH_1708 in contig AE000666_GR.
KEGG	mth:MTH1708.
NMPDR	fig|187420.1.peg.1675.
Phylogenomic databases
eggNOG	arNOG04918.
HOGENOM	HBG539676.
OMA	ICCTAID.
ProtClustDB	PRK00941.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1708-MONOMER.
Family and domain databases
HAMAP	MF_01137. CdhA. [Tree]
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR004460. CO_DH/Ac-CoA_synth_asu. IPR016101. CO_DH_a-bundle. IPR012285. Fum_reductase_C. IPR004137. Prismane. IPR011254. Prismane-like. IPR016099. Prismane-like_a/b-sand. [Graphical view]
Gene3D	G3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit. G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 2 hits.
KO	K00192.
Pfam	PF03063. Prismane. 2 hits. [Graphical view]
SUPFAM	SSF56821. Prismane_like. 1 hit.
TIGRFAMs	TIGR00314. CdhA. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACDA_METTH

Accession

Primary (citable) accession number: O27743

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program