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Protein

Flap endonuclease 1

Gene

fen

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).By similarity

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi27Magnesium 1UniRule annotation1
Metal bindingi80Magnesium 1UniRule annotation1
Metal bindingi152Magnesium 1UniRule annotation1
Metal bindingi154Magnesium 1UniRule annotation1
Metal bindingi173Magnesium 2UniRule annotation1
Metal bindingi175Magnesium 2UniRule annotation1
Metal bindingi226Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMTHE187420:G1G16-1586-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:fenUniRule annotation
Ordered Locus Names:MTH_1633
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540561 – 328Flap endonuclease 1Add BLAST328

Interactioni

Subunit structurei

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1633

Structurei

3D structure databases

ProteinModelPortaliO27670
SMRiO27670
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 98N-domainAdd BLAST98
Regioni116 – 247I-domainAdd BLAST132
Regioni320 – 328Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04050 Archaea
COG0258 LUCA
KOiK04799
OMAiGSQDYDS
OrthoDBiPOG093Z0642

Family and domain databases

CDDicd09867 PIN_FEN1, 1 hit
HAMAPiMF_00614 Fen, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR023426 Flap_endonuc
IPR019973 Flap_structure-sp_endonuc_arc
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit
TIGRFAMsiTIGR03674 fen_arch, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit

Sequencei

Sequence statusi: Complete.

O27670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVKLRDVVS PRRIRLEDLR GRTVAVDAAN TLYQFLSSIR QRDGTPLMDS
60 70 80 90 100
RGRVTSHLSG ILYRTAAVME REIRVIYVFD GRSHHLKGET VSRRADIRKK
110 120 130 140 150
SEVEWKRALE EGDIDRAKKY AVRSSRMSSE ILESSKRLLE LLGIPYVQAP
160 170 180 190 200
GEGEAQASYM VKMGDAWAVA SQDYDCLLFG APRVVRNLTL SGKLEDPEII
210 220 230 240 250
ELESTLRELS ISHTQLVDMA LLVGTDFNEG VKGIGARRGL KLIREKGDIF
260 270 280 290 300
KVIRDLEADI GGDPQVLRRI FLEPEVSEDY EIRWRKPDVE GVIEFLCTEH
310 320
GFSEDRVRAA LKKFEGASST QKSLEDWF
Length:328
Mass (Da):37,170
Last modified:January 1, 1998 - v1
Checksum:iCAC1C6046561FDE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB86106.1
PIRiC69085
RefSeqiWP_010877241.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaiAAB86106; AAB86106; MTH_1633
GeneIDi1470718
KEGGimth:MTH_1633
PATRICifig|187420.15.peg.1597

Similar proteinsi

Entry informationi

Entry nameiFEN_METTH
AccessioniPrimary (citable) accession number: O27670
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health