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O27661 (TOP1_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:MTH_1624
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Magnesium
Metal-binding
Zinc
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145179

Regions

Domain9 – 151143Toprim
Zinc finger598 – 62629C4-type 1 HAMAP-Rule MF_00952
Zinc finger680 – 70627C4-type 2 HAMAP-Rule MF_00952
Region202 – 2076Interaction with DNA By similarity

Sites

Active site3201O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding151Magnesium 1; catalytic By similarity
Metal binding1131Magnesium 1; catalytic By similarity
Metal binding1131Magnesium 2 By similarity
Metal binding1151Magnesium 2 By similarity
Site591Interaction with DNA By similarity
Site1721Interaction with DNA By similarity
Site1761Interaction with DNA By similarity
Site3221Interaction with DNA By similarity
Site5051Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
O27661 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 88507CAED59C0307

FASTA71881,521
        10         20         30         40         50         60 
MRSLSGKMHE VIICEKPKSS EKIAGALFPD AMKKKHGKVS YWEHVEGDKR VTIVSAVGHL 

        70         80         90        100        110        120 
YSLRPRQSNE EHFFDLEWAP IHEIDKKKGY VKDYLNVIRK FAAGADRYIH ACDYDIEGTL 

       130        140        150        160        170        180 
IGFNALKYGC GEEALRKTSR MKFSTLTREE IQRAYKNPIE VDYGQVDSGA ARHILDFIFG 

       190        200        210        220        230        240 
VNISRSLMKS VKAATNRFIK LSAGRVQTPT LAILVEREKE IRDFKPVPYW IIRAELGEGI 

       250        260        270        280        290        300 
IAESKRGKIF KRELVDSILE KCQGSDAEVK DVRVRDTIRK PPVPFDLGTL QSEAYRVFGF 

       310        320        330        340        350        360 
SPKKTQTIAQ NLYTEGYTSY PRTSSQKLPE SIGYEKILKN LAKNPRFGVH IERLRGPLKP 

       370        380        390        400        410        420 
HEGKKEDDAH PAIHPTGLLP SELSKDEKKV YDLIVHRFIS VFGEDAILQT MKVELEIGEE 

       430        440        450        460        470        480 
EFSFSRKRVS KAGWMESYPY TKMEDEEFPE ISGGDSLAVR SVSADERETK PPARYNEASL 

       490        500        510        520        530        540 
IRELERRGLG TKSTRADIIA KLYDRKYIEG KKIRVSPLGE NIIDTLTRYC EKIISEELTR 

       550        560        570        580        590        600 
QFERELEDIM RGKISKDRVI DEAITEVRSI LSDIEENLRD IGKELYRAYQ DSRVVGECPA 

       610        620        630        640        650        660 
CGGKLVIKYS PRNRSTFVGC SSYPDCRTVY SLPRGASVLK SLCEKCGLPM ISYGRPRQRA 

       670        680        690        700        710 
CLDPKCGKKK SEVEEVVGKC PECGSDLIKR SGRYGEFVGC KGFPKCRFTC SVDEVPEG 

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB86097.1.
PIRA69084.
RefSeqNP_276736.1. NC_000916.1.

3D structure databases

ProteinModelPortalO27661.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187420.MTH1624.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB86097; AAB86097; MTH_1624.
GeneID1470709.
KEGGmth:MTH1624.

Phylogenomic databases

eggNOGCOG0551.
KOK03168.
OMAKEYNNWQ.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-1626-MONOMER.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01057. topA_arch. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_METTH
AccessionPrimary (citable) accession number: O27661
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families