O27661 (TOP1_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	MTH_1624

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	DNA topoisomerase 1		PRO_0000145179
Regions
	Domain	9 – 144	136	Toprim
	Zinc finger	598 – 626	29	C4-type 1
	Zinc finger	680 – 706	27	C4-type 2
Sites
	Active site	320	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27661 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 88507CAED59C0307	FASTA	718	81,521
	10 20 30 40 50 60 MRSLSGKMHE VIICEKPKSS EKIAGALFPD AMKKKHGKVS YWEHVEGDKR VTIVSAVGHL 70 80 90 100 110 120 YSLRPRQSNE EHFFDLEWAP IHEIDKKKGY VKDYLNVIRK FAAGADRYIH ACDYDIEGTL 130 140 150 160 170 180 IGFNALKYGC GEEALRKTSR MKFSTLTREE IQRAYKNPIE VDYGQVDSGA ARHILDFIFG 190 200 210 220 230 240 VNISRSLMKS VKAATNRFIK LSAGRVQTPT LAILVEREKE IRDFKPVPYW IIRAELGEGI 250 260 270 280 290 300 IAESKRGKIF KRELVDSILE KCQGSDAEVK DVRVRDTIRK PPVPFDLGTL QSEAYRVFGF 310 320 330 340 350 360 SPKKTQTIAQ NLYTEGYTSY PRTSSQKLPE SIGYEKILKN LAKNPRFGVH IERLRGPLKP 370 380 390 400 410 420 HEGKKEDDAH PAIHPTGLLP SELSKDEKKV YDLIVHRFIS VFGEDAILQT MKVELEIGEE 430 440 450 460 470 480 EFSFSRKRVS KAGWMESYPY TKMEDEEFPE ISGGDSLAVR SVSADERETK PPARYNEASL 490 500 510 520 530 540 IRELERRGLG TKSTRADIIA KLYDRKYIEG KKIRVSPLGE NIIDTLTRYC EKIISEELTR 550 560 570 580 590 600 QFERELEDIM RGKISKDRVI DEAITEVRSI LSDIEENLRD IGKELYRAYQ DSRVVGECPA 610 620 630 640 650 660 CGGKLVIKYS PRNRSTFVGC SSYPDCRTVY SLPRGASVLK SLCEKCGLPM ISYGRPRQRA 670 680 690 700 710 CLDPKCGKKK SEVEEVVGKC PECGSDLIKR SGRYGEFVGC KGFPKCRFTC SVDEVPEG

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB86097.1.
PIR	A69084.
RefSeq	NP_276736.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27661.
ModBase	Search...
Protein-protein interaction databases
STRING	O27661.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1470709.
GenomeReviews	Gene locus MTH_1624 in contig AE000666_GR.
KEGG	mth:MTH1624.
NMPDR	fig|187420.1.peg.1591.
Phylogenomic databases
eggNOG	arNOG04219.
HOGENOM	HBG512935.
OMA	QVIDLVW.
PhylomeDB	O27661.
ProtClustDB	CLSK2517839.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1624-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005739. TopoI_arch. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01057. TopA_arch. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_METTH

Accession

Primary (citable) accession number: O27661

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program