Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 1

Gene

topA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi15 – 151Magnesium 1; catalyticUniRule annotation
Sitei59 – 591Interaction with DNAUniRule annotation
Metal bindingi113 – 1131Magnesium 1; catalyticUniRule annotation
Metal bindingi113 – 1131Magnesium 2UniRule annotation
Metal bindingi115 – 1151Magnesium 2UniRule annotation
Sitei172 – 1721Interaction with DNAUniRule annotation
Sitei176 – 1761Interaction with DNAUniRule annotation
Active sitei320 – 3201O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei322 – 3221Interaction with DNAUniRule annotation
Sitei505 – 5051Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri598 – 62629C4-type 1Add
BLAST
Zinc fingeri680 – 70627C4-type 2Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1626-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:MTH_1624
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718DNA topoisomerase 1PRO_0000145179Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1624.

Structurei

3D structure databases

ProteinModelPortaliO27661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 151143ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 2076Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri598 – 62629C4-type 1Add
BLAST
Zinc fingeri680 – 70627C4-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
KOiK03168.
OMAiPPKRYTM.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01057. topA_arch. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27661-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLSGKMHE VIICEKPKSS EKIAGALFPD AMKKKHGKVS YWEHVEGDKR
60 70 80 90 100
VTIVSAVGHL YSLRPRQSNE EHFFDLEWAP IHEIDKKKGY VKDYLNVIRK
110 120 130 140 150
FAAGADRYIH ACDYDIEGTL IGFNALKYGC GEEALRKTSR MKFSTLTREE
160 170 180 190 200
IQRAYKNPIE VDYGQVDSGA ARHILDFIFG VNISRSLMKS VKAATNRFIK
210 220 230 240 250
LSAGRVQTPT LAILVEREKE IRDFKPVPYW IIRAELGEGI IAESKRGKIF
260 270 280 290 300
KRELVDSILE KCQGSDAEVK DVRVRDTIRK PPVPFDLGTL QSEAYRVFGF
310 320 330 340 350
SPKKTQTIAQ NLYTEGYTSY PRTSSQKLPE SIGYEKILKN LAKNPRFGVH
360 370 380 390 400
IERLRGPLKP HEGKKEDDAH PAIHPTGLLP SELSKDEKKV YDLIVHRFIS
410 420 430 440 450
VFGEDAILQT MKVELEIGEE EFSFSRKRVS KAGWMESYPY TKMEDEEFPE
460 470 480 490 500
ISGGDSLAVR SVSADERETK PPARYNEASL IRELERRGLG TKSTRADIIA
510 520 530 540 550
KLYDRKYIEG KKIRVSPLGE NIIDTLTRYC EKIISEELTR QFERELEDIM
560 570 580 590 600
RGKISKDRVI DEAITEVRSI LSDIEENLRD IGKELYRAYQ DSRVVGECPA
610 620 630 640 650
CGGKLVIKYS PRNRSTFVGC SSYPDCRTVY SLPRGASVLK SLCEKCGLPM
660 670 680 690 700
ISYGRPRQRA CLDPKCGKKK SEVEEVVGKC PECGSDLIKR SGRYGEFVGC
710
KGFPKCRFTC SVDEVPEG
Length:718
Mass (Da):81,521
Last modified:January 1, 1998 - v1
Checksum:i88507CAED59C0307
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB86097.1.
PIRiA69084.
RefSeqiNP_276736.1. NC_000916.1.
WP_010877232.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB86097; AAB86097; MTH_1624.
GeneIDi1470709.
KEGGimth:MTH1624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB86097.1.
PIRiA69084.
RefSeqiNP_276736.1. NC_000916.1.
WP_010877232.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1624.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB86097; AAB86097; MTH_1624.
GeneIDi1470709.
KEGGimth:MTH1624.

Phylogenomic databases

eggNOGiCOG0551.
KOiK03168.
OMAiPPKRYTM.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1626-MONOMER.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01057. topA_arch. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiTOP1_METTH
AccessioniPrimary (citable) accession number: O27661
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 1, 1998
Last modified: April 29, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.