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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NADUniRule annotation1
Binding sitei63NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei127NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi155Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi170IronUniRule annotation1
Binding sitei224NAD; via amide nitrogenUniRule annotation1
Binding sitei234GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 56NADUniRule annotation2
Nucleotide bindingi153 – 155NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:MTH_1620
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539501 – 258Thiamine thiazole synthaseAdd BLAST258

Proteomic databases

PRIDEiO27657.

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1620.

Structurei

3D structure databases

ProteinModelPortaliO27657.
SMRiO27657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG0940010T.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

O27657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDDIKISR AIVEGYMEDL LDYMEMDVAI GGGGPSGLTA GYYLARAGLK
60 70 80 90 100
VALFERKLSI GGGMWGGGMM FNKIVVQDEG REILDEFGIR SEPYDEGYHV
110 120 130 140 150
ADSVEATSTL CSRACQAGLK IFNLMSIEDV MIRDEGITGL VLNWSSVEMA
160 170 180 190 200
GLHVDPLTVR ARAVIDATGH DCEIVKVVER KIGPELNTPD GRIQGERSMW
210 220 230 240 250
ADVGEAALIE NTREVYPNLY VAGMASNAVY GAPRMGPIFG GMLVSGRRVA

EMIIEKLK
Length:258
Mass (Da):28,043
Last modified:October 31, 2006 - v2
Checksum:iFA11B3320F2DCFCA
GO

Sequence cautioni

The sequence AAB86093 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB86093.1. Different initiation.
PIRiE69083.
RefSeqiWP_048061316.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB86093; AAB86093; MTH_1620.
GeneIDi24854722.
KEGGimth:MTH_1620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB86093.1. Different initiation.
PIRiE69083.
RefSeqiWP_048061316.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27657.
SMRiO27657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1620.

Proteomic databases

PRIDEiO27657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB86093; AAB86093; MTH_1620.
GeneIDi24854722.
KEGGimth:MTH_1620.

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG0940010T.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_METTH
AccessioniPrimary (citable) accession number: O27657
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: October 31, 2006
Last modified: February 15, 2017
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.