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O27642 (DUT_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
dUTP pyrophosphatase
Gene names
Name:dut
Ordered Locus Names:MTH_1605
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00635

Catalytic activity

dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00635

Pathway

Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00635

Sequence similarities

Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.

Ontologies

Keywords
   Biological processNucleotide metabolism
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdUMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

dUTP metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 150150Probable deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00635
PRO_0000153641

Sequences

Sequence LengthMass (Da)Tools
O27642 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: AFA09D55FB371648

FASTA15016,997
        10         20         30         40         50         60 
MMIGEQLLKK LFPDFEELVQ PAGIDLRVDK VYRQMGPGSL IDDEKNLPPL EMLEPPIYRL 

        70         80         90        100        110        120 
EPGKAYLASV DRMIEIPEGY AMLYLPRSTL LRSFVSVQTA VGDPGFRGTL QFLLHNHGEY 

       130        140        150 
EYTLKRGERI VQAVVFPVEG SGKYSGSYQE 

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB86078.1.
PIRD69081.
RefSeqNP_276717.1. NC_000916.1.

3D structure databases

ProteinModelPortalO27642.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187420.MTH1605.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB86078; AAB86078; MTH_1605.
GeneID1471874.
KEGGmth:MTH1605.

Phylogenomic databases

eggNOGCOG0717.
KOK01520.
OMAGEYNGSY.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-1607-MONOMER.
UniPathwayUPA00610; UER00666.

Family and domain databases

Gene3D2.70.40.10. 1 hit.
HAMAPMF_00635. dUTPase_arch.
InterProIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR023537. dUTPase_archaeal.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMSSF51283. SSF51283. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDUT_METTH
AccessionPrimary (citable) accession number: O27642
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways