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Protein

Glutamine synthetase

Gene

glnA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi133Magnesium 1By similarity1
Metal bindingi135Magnesium 2By similarity1
Binding sitei183ATPBy similarity1
Metal bindingi188Magnesium 2By similarity1
Metal bindingi195Magnesium 2By similarity1
Binding sitei240L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi244Magnesium 1; via pros nitrogenBy similarity1
Binding sitei248ATPBy similarity1
Binding sitei297L-glutamateBy similarity1
Binding sitei303L-glutamateBy similarity1
Binding sitei315ATPBy similarity1
Binding sitei315L-glutamateBy similarity1
Binding sitei320ATPBy similarity1
Binding sitei327ATPBy similarity1
Metal bindingi332Magnesium 1By similarity1
Binding sitei334L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi246 – 248ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:MTH_1570
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532061 – 442Glutamine synthetaseAdd BLAST442

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi187420.MTH1570.

Structurei

3D structure databases

ProteinModelPortaliO27612.
SMRiO27612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni239 – 240L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
KOiK01915.
OMAiIEAAWNT.
OrthoDBiPOG093Z00GE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

O27612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKIGRIIA KMDECGVKFV RLQFVDIHGK PKNMAIPLVR PDQIEDIIKD
60 70 80 90 100
GLLFDGSSVE GFVDINESDL VLKPDPDTFS TLPWRPEEKG VCRFICDIYW
110 120 130 140 150
PDGKPFEGDP RYVLKRALDK YAHLGYEYNV GPEPEFFILD QDEDGNIIPH
160 170 180 190 200
DCGAYFDVEP VDQGTDFRRK LVMDLEALNF DVEVSHHEVA PGQHEIDFKF
210 220 230 240 250
DKALKTADAV ITFKQAIKAI VDKIGYMVTF MPKPFFGENG SGMHCHQSLF
260 270 280 290 300
KDGENVFYDP DTETQLSEEA LYFIGGLLKH APALTAVCAP TVNSYKRLVP
310 320 330 340 350
GYEAPVYIAY GLKNRSTLIR IPASRGKGTR VELRMPDPSC NPYLAFAAML
360 370 380 390 400
EAGMNGIQNK IDPGEPTEID VYEKSMSELR EMGIETLPSS LWEAYHALEE
410 420 430 440
DDVIKGALGG HVYEKFMEIK HREWDDYRVR VFKYELERYL DI
Length:442
Mass (Da):50,248
Last modified:January 1, 1998 - v1
Checksum:iB907928AAD8960A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB86044.1.
PIRiF69076.
RefSeqiWP_010877179.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB86044; AAB86044; MTH_1570.
GeneIDi1471839.
KEGGimth:MTH_1570.
PATRICifig|187420.15.peg.1533.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1A_METTH
AccessioniPrimary (citable) accession number: O27612
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families