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Protein

Nitrogenase iron protein 1

Gene

nifH1

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.By similarity

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per dimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1
Metal bindingi132Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 16ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:G1G16-1513-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron protein 1 (EC:1.18.6.1)
Alternative name(s):
Nitrogenase Fe protein 1
Nitrogenase component II
Nitrogenase reductase
Gene namesi
Name:nifH1
Ordered Locus Names:MTH_1560
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395421 – 275Nitrogenase iron protein 1Add BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei100ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseBy similarity1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-100 inactivates the nitrogenase reductase and regulates nitrogenase activity.By similarity

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi187420.MTH1560

Structurei

3D structure databases

ProteinModelPortaliO27602
SMRiO27602
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.Curated

Phylogenomic databases

eggNOGiarCOG00590 Archaea
COG1348 LUCA
KOiK02588
OMAiEMGQRIM
OrthoDBiPOG093Z00RZ

Family and domain databases

CDDicd02040 NifH, 1 hit
HAMAPiMF_00533 NifH, 1 hit
InterProiView protein in InterPro
IPR030655 NifH/chlL_CS
IPR000392 NifH/frxC
IPR005977 Nitrogenase_Fe_NifH
IPR027417 P-loop_NTPase
PANTHERiPTHR42864 PTHR42864, 1 hit
PfamiView protein in Pfam
PF00142 Fer4_NifH, 1 hit
PIRSFiPIRSF000363 Nitrogenase_iron, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01287 nifH, 1 hit
PROSITEiView protein in PROSITE
PS00746 NIFH_FRXC_1, 1 hit
PS00692 NIFH_FRXC_2, 1 hit
PS51026 NIFH_FRXC_3, 1 hit

Sequencei

Sequence statusi: Complete.

O27602-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRKIAIYGK GGIGKSTTQQ NTAAAMSYFH GKNVMIHGCD PKADSTRLIL
60 70 80 90 100
GGKMQTTMMD TLRELGEVAC TPDKVIETGF GGIKCVESGG PEPGVGCAGR
110 120 130 140 150
GVITAITLME RHGVYEKDLD FVFFDVLGDV VCGGFAMPVR DGKAEEIYIV
160 170 180 190 200
ASGEMMALYA ANNICKGMVK YARQSGVRLG GIICNSRNVD GEKELLEEFC
210 220 230 240 250
ERIGTQMIHF VPRDNIVQKA EFNKKSVIEF DPECNQSQEY RELARKIIEN
260 270
KDFVIPEPMT MDEMEELVVK YGVMD
Length:275
Mass (Da):30,280
Last modified:April 3, 2002 - v2
Checksum:i250277C18094B02D
GO

Sequence cautioni

The sequence AAB86034 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB86034.1 Different initiation.
PIRiC69075
RefSeqiWP_048061088.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaiAAB86034; AAB86034; MTH_1560
GeneIDi1471829
KEGGimth:MTH_1560
PATRICifig|187420.15.peg.1523

Similar proteinsi

Entry informationi

Entry nameiNIFH1_METTH
AccessioniPrimary (citable) accession number: O27602
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: April 3, 2002
Last modified: March 28, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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