Reviewed, UniProtKB/Swiss-Prot O27600 (FWDC_METTH)

Names and origin

Protein names

Recommended name:
    Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C
    EC=1.2.99.5
Alternative name(s):
    Tungsten-containing formylmethanofuran dehydrogenase II subunit C

Gene names

Name:	fwdC
Ordered Locus Names:	MTH_1558

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of CO2 and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). Can only oxidize formylmethanofuran. This enzyme is oxygen-labile.
Catalytic activity	Formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor.
Cofactor	Tungsten.
Enzyme regulation	Not inactivated by cyanide.
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 1/3.
Subunit structure	This enzyme is composed of seven subunits fwdA (65 kDa), fwdB (53 kDa), fwdC (31 kDa), fwdD (15 kDa), fwdE, fwdF and fwdG By similarity.
Induction	By growth on tungsten or molybdenum under anaerobic conditions.
Sequence similarities	Belongs to the fwdC/fmdC family.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	Tungsten
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	formylmethanofuran dehydrogenase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C		PRO_0000144196
Regions
	Repeat	80 – 92	13	1
	Repeat	99 – 111	13	2
	Repeat	118 – 130	13	3
	Repeat	144 – 156	13	4
	Repeat	163 – 175	13	5
	Repeat	182 – 194	13	6
	Repeat	201 – 213	13	7
	Region	80 – 213	134	7 X 13 AA repeats of [GW]-X-X-M-X-X-G-X-[IL]-X-[IV]-X-G

Sequences

	Sequence		Length	Mass (Da)
	O27600-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 043A9FFBBA554D36	FASTA	270	28,641
	10 20 30 40 50 60 MSEIILTPKE QPEVPLEAPN IKPDVFAGKS IDEIRNIQIM HGNEVVKLGD FFEVSGEPAD 70 80 90 100 110 120 SAADIKIIID GDVYNTKRIG QDMTAGEILV KGNVNMYVGA GMKGGKITVE GNAKSWAGQD 130 140 150 160 170 180 MRGGELEIFG DAGDYVGSSY RGDWRGMSGG VITVHGNAGN EIGEYMNGGK IIIKGDVNIM 190 200 210 220 230 240 PGIHMNNGLI IIEGNAVARV GGEMAGGTIV VKGMIEEFLP GFKYLGVEKD IEVNGETFPG 250 260 270 AYYKFEGDHA IKGAKGMVYA AVGCNGHIEP

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB86032.1.
PIR	H69074.
RefSeq	NP_276671.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.8.1.1. Na+- or H+-pumping formyl methanofuran dehydrogenase (FMF-DH) family.
Genome annotation databases
GeneID	1471827.
GenomeReviews	Gene locus MTH_1558 in contig AE000666_GR.
KEGG	mth:MTH1558.
NMPDR	fig|187420.1.peg.1526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O27600.
OMA	O27600. YVGSAYR.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1558-MON.
BRENDA	1.2.99.5. 270.
Family and domain databases
InterPro	IPR017550. Formylmethanofuran_DH_suC. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF01493. GXGXG. 1 hit. [Graphical view]
TIGRFAMs	TIGR03122. one_C_dehyd_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FWDC_METTH

Accession

Primary (citable) accession number: O27600

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)