Reviewed, UniProtKB/Swiss-Prot O27579 (DP2L_METTH)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MTH_1536

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	1092 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1092	1092	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000152578

Sequences

	Sequence		Length	Mass (Da)
	O27579-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: AA6970F7A6F42DFF	FASTA	1,092	123,060
	10 20 30 40 50 60 MMDYFNELER ETERLYEIAR KARARGLDVS TTPEIPLAKD LAERVEGLVG PEGIARRIKE 70 80 90 100 110 120 LEGDRGREEV AFQIAAEIAS QAVPDDDPEE REKLADQALR TALAILTEGV VAAPLEGIAR 130 140 150 160 170 180 VRIKENFDKS RYLAVYFAGP IRSAGGTAAA LSVLIADYIR LAVGLDRYKP VEREIERYVE 190 200 210 220 230 240 EVELYESEVT NLQYSPKPDE VRLAASKIPV EVTGEPTDKV EVSHRDLERV ETNNIRGGAL 250 260 270 280 290 300 LAMVEGVIQK APKVLKYAKQ LKLEGWDWLE KFSKAPKKGE GEEKVVVKAD SKYVEDIIGG 310 320 330 340 350 360 RPVLAYPSEK GAFRLRYGRA RNTGLAAMGV HPATMELLQF LAVGTQMKIE RPGKGNCVVP 370 380 390 400 410 420 VDTIDGPVVK LRNGDVIRIE DAETASRVRS EVEEILFLGD MLVAFGEFLR NNHVLMPAGW 430 440 450 460 470 480 CEEWWIQTIL SSPKYPGDDP LNLSYYRTRW NELEVSAGDA FRISEEYDVP LHPRYTYFYH 490 500 510 520 530 540 DVTVRELNML REWLNTSQLE DELVLELRPE KRILEILGVP HRVKDSRVVI GHDDAHALIK 550 560 570 580 590 600 TLRKPLEDSS DTVEALNRVS PVRIMKKAPT YIGTRVGRPE KTKERKMRPA PHVLFPIGKY 610 620 630 640 650 660 GGSRRNIPDA AKKGSITVEI GRATCPSCRV SSMQSICPSC GSRTVIGEPG KRNINLAALL 670 680 690 700 710 720 KRAAENVSVR KLDEIKGVEG MISAEKFPEP LEKGILRAKN DVYTFKDATI RHDSTDLPLT 730 740 750 760 770 780 HFTPREVGVS VERLRELGYT RDCYGDELED EDQILELRVQ DVVISEDCAD YLVRVANFVD 790 800 810 820 830 840 DLLERFYDLE RFYNVKTRED LVGHLIAGLA PHTSAAVLGR IIGFTGASAC YAHPYFHSAK 850 860 870 880 890 900 RRNCDSDEDS VMLLLDALLN FSKSYLPSSR GGSMDAPLVL STRIDPEEID DESHNIDTMD 910 920 930 940 950 960 MIPLEVYERS FDHPRPSEVL DVIDNVEKRL GKPEQYTGLM FSHNTSRIDE GPKVCLYKLL 970 980 990 1000 1010 1020 PTMKEKVESQ ITLAEKIRAV DQRSVVEGVL MSHFLPDMMG NIRAFSRQKV RCTKCNRKYR 1030 1040 1050 1060 1070 1080 RIPLSGECRC GGNLVLTVSK GSVIKYLEIS KELASRYPID PYLMQRIEIL EYGVNSLFES 1090 DRSKQSSLDV FL

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB86010.1.
PIR	H69071.
RefSeq	NP_276649.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1471805.
GenomeReviews	Gene locus MTH_1536 in contig AE000666_GR.
KEGG	mth:MTH1536.
NMPDR	fig|187420.1.peg.1504.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O27579.
OMA	O27579. NCDGDED.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1536-MON.
BRENDA	2.7.7.7. 270.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METTH

Accession

Primary (citable) accession number: O27579

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 1, 1998
Last modified:	May 5, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)