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Protein

Imidazole glycerol phosphate synthase subunit HisH

Gene

hisH

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR (By similarity).By similarity

Catalytic activityi

5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Phosphoribosyl-ATP pyrophosphatase (hisE)
  3. Phosphoribosyl-AMP cyclohydrolase (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. no protein annotated in this organism
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei77NucleophileBy similarity1
Active sitei177By similarity1
Active sitei179By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00031; UER00010.

Protein family/group databases

MEROPSiC26.965.

Names & Taxonomyi

Protein namesi
Recommended name:
Imidazole glycerol phosphate synthase subunit HisH (EC:2.4.2.-)
Alternative name(s):
IGP synthase glutamine amidotransferase subunit
IGP synthase subunit HisH
ImGP synthase subunit HisH
Short name:
IGPS subunit HisH
Gene namesi
Name:hisH
Ordered Locus Names:MTH_1524
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001524631 – 198Imidazole glycerol phosphate synthase subunit HisHAdd BLAST198

Proteomic databases

PRIDEiO27568.

Interactioni

Subunit structurei

Heterodimer of HisH and HisF.By similarity

Protein-protein interaction databases

STRINGi187420.MTH1524.

Structurei

3D structure databases

ProteinModelPortaliO27568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 198Glutamine amidotransferase type-1Add BLAST198

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00089. Archaea.
COG0118. LUCA.
KOiK02501.
OMAiGMQMLLT.

Family and domain databases

CDDicd01748. GATase1_IGP_Synthase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00278. HisH. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010139. Imidazole-glycPsynth_HisH.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
PIRSFiPIRSF000495. Amidotransf_hisH. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01855. IMP_synth_hisH. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27568-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAIIDYGSG NLRSIANAFR KIGADALVTS DPQILEAADA LVLPGVGAFG
60 70 80 90 100
SAMAKLEGLR ETLLGNIMDG KPFLGICLGL QVLLSESQES PGVHGLDLIP
110 120 130 140 150
GRVIRIPPGN KVPHMGWNQL IIVEDSQLLE GAEDEYFYFV HSYYAEPSND
160 170 180 190
VVVARTDYGV EMTAAIESDN IHATQFHPEK SGEAGLDVLR NFVEIIRA
Length:198
Mass (Da):21,348
Last modified:January 1, 1998 - v1
Checksum:iE8EFA43EC9163AEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85999.1.
PIRiD69070.
RefSeqiWP_010877134.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85999; AAB85999; MTH_1524.
GeneIDi1471793.
KEGGimth:MTH_1524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85999.1.
PIRiD69070.
RefSeqiWP_010877134.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1524.

Protein family/group databases

MEROPSiC26.965.

Proteomic databases

PRIDEiO27568.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85999; AAB85999; MTH_1524.
GeneIDi1471793.
KEGGimth:MTH_1524.

Phylogenomic databases

eggNOGiarCOG00089. Archaea.
COG0118. LUCA.
KOiK02501.
OMAiGMQMLLT.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00010.

Family and domain databases

CDDicd01748. GATase1_IGP_Synthase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00278. HisH. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010139. Imidazole-glycPsynth_HisH.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
[Graphical view]
PIRSFiPIRSF000495. Amidotransf_hisH. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01855. IMP_synth_hisH. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIS5_METTH
AccessioniPrimary (citable) accession number: O27568
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.