O27554 (NADE_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	MTH_1510

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.
Sequence caution	The sequence AAB85985.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Probable NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152227
Regions
	Nucleotide binding	34 – 41	8	ATP By similarity
Sites
	Active site	36	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27554 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 5E978197082CC145	FASTA	266	28,722
	10 20 30 40 50 60 MKCVPGLCSE VVSVIEDFIR QKVAESGASG VVLGLSGGVD SSTVAYLAVN ALGPDRVLGL 70 80 90 100 110 120 IMPSSTTPRD DLRHARTVAD ELGIESETID IDPIIESLTG LCSHNANELA LANLKPRARM 130 140 150 160 170 180 VILYYHANSL NRLVAGTGNR TELLLGYFTK YGDGGVDMLP IGGLYKGQVR ELAGRLGVPP 190 200 210 220 230 240 EIIKKPPTAG LWHGQTDEEE LGMKYDLLDE LLCLLVDRKL PVEEVASTLS LPPSEVERIA 250 260 SMVKGSEHKL KPPEVPDIWE VMECSH

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85985.1. Different initiation.
PIR	E69068.
RefSeq	NP_276624.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27554.
SMR	O27554. Positions 16-258.
ModBase	Search...
Protein-protein interaction databases
STRING	O27554.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471779.
GenomeReviews	Gene locus MTH_1510 in contig AE000666_GR.
KEGG	mth:MTH1510.
NMPDR	fig|187420.1.peg.1479.
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	DGAVDCH.
PhylomeDB	O27554.
ProtClustDB	CLSK2750597.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1510-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_METTH

Accession

Primary (citable) accession number: O27554

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 30, 2000
Last modified:	November 16, 2011
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program