Reviewed, UniProtKB/Swiss-Prot O27544 (RIFK_METTH)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	MTH_1500

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 122	122	Riboflavin kinase HAMAP MF_01285		PRO_0000322068
Regions
	Nucleotide binding	4 – 9	6	CDP By similarity
	Nucleotide binding	97 – 100	4	CDP By similarity
Sites
	Metal binding	33	1	Magnesium By similarity
	Metal binding	35	1	Magnesium By similarity
	Binding site	84	1	FMN By similarity
	Binding site	92	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27544-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 43F88A920488B8CC	FASTA	122	13,438
	10 20 30 40 50 60 MKSGFGEGAY FITRSVYRDQ FREKLGFDPF PGTLNIEVGD PEIVERIREG APVIQGGGGF 70 80 90 100 110 120 GDVLYVKALL NGVVEGAILF PLKTHHRQGC LEFVAPVNLR KTLKLRDGDT VSLDIDTSEI QE

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB85975.1.
PIR	B69067.
RefSeq	NP_276614.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O27544.
Genome annotation databases
GeneID	1471769.
GenomeReviews	Gene locus MTH_1500 in contig AE000666_GR.
KEGG	mth:MTH1500.
NMPDR	fig|187420.1.peg.1469.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O27544.
OMA	KTTHEEN.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1500-MON.
Family and domain databases
HAMAP	MF_01285. [Tree]
InterPro	IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_METTH

Accession

Primary (citable) accession number: O27544

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)