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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73Carbamoyl phosphateUniRule annotation1
Binding sitei97Carbamoyl phosphateUniRule annotation1
Binding sitei154OrnithineUniRule annotation1
Binding sitei218OrnithineUniRule annotation1
Binding sitei286Carbamoyl phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:MTH_1446
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001130691 – 301Ornithine carbamoyltransferaseAdd BLAST301

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH1446.

Structurei

3D structure databases

ProteinModelPortaliO27495.
SMRiO27495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 49Carbamoyl phosphate bindingUniRule annotation4
Regioni124 – 127Carbamoyl phosphate bindingUniRule annotation4
Regioni222 – 223Ornithine bindingUniRule annotation2
Regioni258 – 259Carbamoyl phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiarCOG00912. Archaea.
COG0078. LUCA.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiPOG093Z05BF.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiView protein in InterPro
IPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
PfamiView protein in Pfam
PF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiView protein in PROSITE
PS00097. CARBAMOYLTRANSFERASE. 1 hit.

Sequencei

Sequence statusi: Complete.

O27495-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHLLSVCDM DNVVDLLDLA DDYKEGKIRE KILRGKTLAM IFEKSSTRTR
60 70 80 90 100
VSFEVGAFQM GAQPLYLSAS DLQLGRGEPI ADTARTLSRY VDGIMIRAIS
110 120 130 140 150
HSDVVELAGE ASVPVINGLT DLEHPCQALA DMQTIREKLG GFDGRLVFVG
160 170 180 190 200
DGNNVCHSLL LITATLGMDM DVACPPGYEP DPGIREMAGK IADETGSRIR
210 220 230 240 250
VIHDPSEAVR GADVVYTDVW VSMGYEDEAE DRLEVFRPYQ VNMELMELAA
260 270 280 290 300
PEAIFMHCLP AVRGQETTAE VIDGPHSVVW DQAENRLHAQ KAIMHWLMGD

I
Length:301
Mass (Da):33,186
Last modified:January 1, 1998 - v1
Checksum:i2B688E2A1C58ECB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85921.1.
PIRiE69059.
RefSeqiWP_010877056.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85921; AAB85921; MTH_1446.
GeneIDi24854557.
KEGGimth:MTH_1446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85921.1.
PIRiE69059.
RefSeqiWP_010877056.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27495.
SMRiO27495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1446.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85921; AAB85921; MTH_1446.
GeneIDi24854557.
KEGGimth:MTH_1446.

Phylogenomic databases

eggNOGiarCOG00912. Archaea.
COG0078. LUCA.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiPOG093Z05BF.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiView protein in InterPro
IPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
PfamiView protein in Pfam
PF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiView protein in PROSITE
PS00097. CARBAMOYLTRANSFERASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_METTH
AccessioniPrimary (citable) accession number: O27495
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: March 15, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.