Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O27494

- PUR2_METTH

UniProt

O27494 - PUR2_METTH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi276 – 2761Magnesium or manganese 1UniRule annotation
Metal bindingi288 – 2881Magnesium or manganese 1UniRule annotation
Metal bindingi288 – 2881Magnesium or manganese 2UniRule annotation
Metal bindingi290 – 2901Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 19664ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1447-MONOMER.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:MTH_1445
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436Phosphoribosylamine--glycine ligasePRO_0000151513Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH1445.

Structurei

3D structure databases

ProteinModelPortaliO27494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini106 – 318213ATP-graspUniRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi44 – 496Poly-Leu

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0151.
KOiK01945.
OMAiYKGFLYA.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27494 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKILVVGTGA REHAICSALA DEATIYSVMG NRNPGISRLA GNSLLLLKLT
60 70 80 90 100
PKVVRFASEK GVDMAFIGPE AHLEAGLVDA LEEAGIPSVG PTRDAARIET
110 120 130 140 150
DKSFMRKLFE DYRIPGSITY RVFSDPEELR EFMEDFDSEA VVKPVGLTGG
160 170 180 190 200
KGVKIVGEHL RDNMEALKYA TEVIEKRIGG HPSVVIEERV VGEEFTVQAF
210 220 230 240 250
SDGEHIVPMP AVQDHPHAYE GDQGPITGGM GSYSDSDGLL PFLTQKDYED
260 270 280 290 300
AVEIMQRTVD AIRKETGPYR GILYGQFMLS ADGPKLIEYN ARFGDPEAMN
310 320 330 340 350
VLPLLESSML EICEGIVDGN LKSASFMNLA TVCKYLVPEG YPESGVAGAE
360 370 380 390 400
IKVDEKKIED MGVITYYAAV NQEDDHIYTS SSRALALVAP ADDIYSAEEL
410 420 430
CEEATAHVKG RLYHRRDIGT RELVEKRIKH MEDLRS
Length:436
Mass (Da):47,882
Last modified:May 30, 2000 - v2
Checksum:iDF36C512561D354D
GO

Sequence cautioni

The sequence AAB85920.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85920.1. Different initiation.
PIRiD69059.
RefSeqiNP_276559.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85920; AAB85920; MTH_1445.
GeneIDi1471714.
KEGGimth:MTH1445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85920.1 . Different initiation.
PIRi D69059.
RefSeqi NP_276559.1. NC_000916.1.

3D structure databases

ProteinModelPortali O27494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH1445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85920 ; AAB85920 ; MTH_1445 .
GeneIDi 1471714.
KEGGi mth:MTH1445.

Phylogenomic databases

eggNOGi COG0151.
KOi K01945.
OMAi YKGFLYA.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
BioCyci MTHE187420:GJNM-1447-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiPUR2_METTH
AccessioniPrimary (citable) accession number: O27494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: October 1, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3