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O27494

- PUR2_METTH

UniProt

O27494 - PUR2_METTH

Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi276 – 2761Magnesium or manganese 1UniRule annotation
    Metal bindingi288 – 2881Magnesium or manganese 1UniRule annotation
    Metal bindingi288 – 2881Magnesium or manganese 2UniRule annotation
    Metal bindingi290 – 2901Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi133 – 19664ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. manganese ion binding Source: UniProtKB-HAMAP
    4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-1447-MONOMER.
    UniPathwayiUPA00074; UER00125.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
    Alternative name(s):
    GARSUniRule annotation
    Glycinamide ribonucleotide synthetaseUniRule annotation
    Phosphoribosylglycinamide synthetaseUniRule annotation
    Gene namesi
    Name:purDUniRule annotation
    Ordered Locus Names:MTH_1445
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 436436Phosphoribosylamine--glycine ligasePRO_0000151513Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi187420.MTH1445.

    Structurei

    3D structure databases

    ProteinModelPortaliO27494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini106 – 318213ATP-graspUniRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi44 – 496Poly-Leu

    Sequence similaritiesi

    Belongs to the GARS family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0151.
    KOiK01945.
    OMAiYKGFLYA.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    HAMAPiMF_00138. GARS.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O27494-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKILVVGTGA REHAICSALA DEATIYSVMG NRNPGISRLA GNSLLLLKLT    50
    PKVVRFASEK GVDMAFIGPE AHLEAGLVDA LEEAGIPSVG PTRDAARIET 100
    DKSFMRKLFE DYRIPGSITY RVFSDPEELR EFMEDFDSEA VVKPVGLTGG 150
    KGVKIVGEHL RDNMEALKYA TEVIEKRIGG HPSVVIEERV VGEEFTVQAF 200
    SDGEHIVPMP AVQDHPHAYE GDQGPITGGM GSYSDSDGLL PFLTQKDYED 250
    AVEIMQRTVD AIRKETGPYR GILYGQFMLS ADGPKLIEYN ARFGDPEAMN 300
    VLPLLESSML EICEGIVDGN LKSASFMNLA TVCKYLVPEG YPESGVAGAE 350
    IKVDEKKIED MGVITYYAAV NQEDDHIYTS SSRALALVAP ADDIYSAEEL 400
    CEEATAHVKG RLYHRRDIGT RELVEKRIKH MEDLRS 436
    Length:436
    Mass (Da):47,882
    Last modified:May 30, 2000 - v2
    Checksum:iDF36C512561D354D
    GO

    Sequence cautioni

    The sequence AAB85920.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85920.1. Different initiation.
    PIRiD69059.
    RefSeqiNP_276559.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85920; AAB85920; MTH_1445.
    GeneIDi1471714.
    KEGGimth:MTH1445.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85920.1 . Different initiation.
    PIRi D69059.
    RefSeqi NP_276559.1. NC_000916.1.

    3D structure databases

    ProteinModelPortali O27494.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 187420.MTH1445.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB85920 ; AAB85920 ; MTH_1445 .
    GeneIDi 1471714.
    KEGGi mth:MTH1445.

    Phylogenomic databases

    eggNOGi COG0151.
    KOi K01945.
    OMAi YKGFLYA.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    BioCyci MTHE187420:GJNM-1447-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    HAMAPi MF_00138. GARS.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiPUR2_METTH
    AccessioniPrimary (citable) accession number: O27494
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3