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O27494

- PUR2_METTH

UniProt

O27494 - PUR2_METTH

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Protein

Phosphoribosylamine--glycine ligase

Gene
purD, MTH_1445
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi276 – 2761Magnesium or manganese 1 By similarity
Metal bindingi288 – 2881Magnesium or manganese 1 By similarity
Metal bindingi288 – 2881Magnesium or manganese 2 By similarity
Metal bindingi290 – 2901Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 19664ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1447-MONOMER.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene namesi
Name:purD
Ordered Locus Names:MTH_1445
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436Phosphoribosylamine--glycine ligaseUniRule annotationPRO_0000151513Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH1445.

Structurei

3D structure databases

ProteinModelPortaliO27494.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini106 – 318213ATP-graspAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi44 – 496Poly-LeuUniRule annotation

Sequence similaritiesi

Belongs to the GARS family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
KOiK01945.
OMAiYKGFLYA.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27494-1 [UniParc]FASTAAdd to Basket

« Hide

MKILVVGTGA REHAICSALA DEATIYSVMG NRNPGISRLA GNSLLLLKLT    50
PKVVRFASEK GVDMAFIGPE AHLEAGLVDA LEEAGIPSVG PTRDAARIET 100
DKSFMRKLFE DYRIPGSITY RVFSDPEELR EFMEDFDSEA VVKPVGLTGG 150
KGVKIVGEHL RDNMEALKYA TEVIEKRIGG HPSVVIEERV VGEEFTVQAF 200
SDGEHIVPMP AVQDHPHAYE GDQGPITGGM GSYSDSDGLL PFLTQKDYED 250
AVEIMQRTVD AIRKETGPYR GILYGQFMLS ADGPKLIEYN ARFGDPEAMN 300
VLPLLESSML EICEGIVDGN LKSASFMNLA TVCKYLVPEG YPESGVAGAE 350
IKVDEKKIED MGVITYYAAV NQEDDHIYTS SSRALALVAP ADDIYSAEEL 400
CEEATAHVKG RLYHRRDIGT RELVEKRIKH MEDLRS 436
Length:436
Mass (Da):47,882
Last modified:May 30, 2000 - v2
Checksum:iDF36C512561D354D
GO

Sequence cautioni

The sequence AAB85920.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85920.1. Different initiation.
PIRiD69059.
RefSeqiNP_276559.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85920; AAB85920; MTH_1445.
GeneIDi1471714.
KEGGimth:MTH1445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85920.1 . Different initiation.
PIRi D69059.
RefSeqi NP_276559.1. NC_000916.1.

3D structure databases

ProteinModelPortali O27494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH1445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85920 ; AAB85920 ; MTH_1445 .
GeneIDi 1471714.
KEGGi mth:MTH1445.

Phylogenomic databases

eggNOGi COG0151.
KOi K01945.
OMAi YKGFLYA.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
BioCyci MTHE187420:GJNM-1447-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiPUR2_METTH
AccessioniPrimary (citable) accession number: O27494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: May 14, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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