O27491 (ILVC_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	MTH_1442

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.
Sequence caution	The sequence AAB85917.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151395
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O27491 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 3A8E7D2B8679866B	FASTA	328	36,494
	10 20 30 40 50 60 MKIYYENDID MEILADKKIA VIGYGSQGEA QARNMADSGL NVIVGLRRGG SSWKKAHDDG 70 80 90 100 110 120 MNVMTIEDAS READIIHILI PDEIQETVFE QSIRPYLKEG NTISFSHGYN IHYGYIKAPE 130 140 150 160 170 180 GVNVTMVAPK GPGAMVRRTY LEGFGIPGLV AVEVDATGDA MEQALAMAKA CGLARAGVLE 190 200 210 220 230 240 TTFREETETD LFGEQAVLCG GVTELINTAF KTLVRAGYQP EIAYFETCHE LKLIVDLIYE 250 260 270 280 290 300 RGFRGMWHNV SNTAEFGGLT RRGRIITEET EKEMDEILKE IQNGKFAKEW ALENRAGAPM 310 320 LKRMRKLESE LEIEEVGSKL RKLCGLEK

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85917.1. Different initiation.
PIR	A69059.
RefSeq	NP_276556.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27491.
ModBase	Search...
Protein-protein interaction databases
STRING	O27491.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471711.
GenomeReviews	Gene locus MTH_1442 in contig AE000666_GR.
KEGG	mth:MTH1442.
NMPDR	fig|187420.1.peg.1411.
Phylogenomic databases
eggNOG	arNOG04335.
HOGENOM	HBG286525.
OMA	MAYAKGI.
PhylomeDB	O27491.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11920. MTHE187420:MTH1442-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_METTH

Accession

Primary (citable) accession number: O27491

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	October 17, 2006
Last modified:	November 16, 2011
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program