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O27476 (KAE1B_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. tRNA N6-adenosine threonylcarbamoyltransferase
    EC=2.3.1.-
    Alternative name(s):
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
  2. Serine/threonine-protein kinase Bud32
    EC=2.7.11.1
Gene names
Ordered Locus Names:MTH_1425
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01447

Subunit structure

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / TsaD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000096980

Regions

Domain335 – 534200Protein kinase
Nucleotide binding340 – 3489ATP By similarity
Region1 – 325325Kae1 HAMAP-Rule MF_01447
Region129 – 1335Threonylcarbamoyl-AMP binding By similarity

Sites

Active site4511Proton acceptor; for kinase activity By similarity
Metal binding1081Iron By similarity
Metal binding1121Iron By similarity
Metal binding1291Iron By similarity
Metal binding2861Iron By similarity
Binding site1611Threonylcarbamoyl-AMP By similarity
Binding site1741Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding site1781Threonylcarbamoyl-AMP By similarity
Binding site2581Threonylcarbamoyl-AMP By similarity
Binding site3611ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O27476 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 5DD30415047EE087

FASTA53458,298
        10         20         30         40         50         60 
MLCLGIEGTA EKTGVGIVDE AGNVLSLRGK PLIPEKGGIH PREAAEHHAK WIPRLIAEAC 

        70         80         90        100        110        120 
RDAGVELGEI GLISFSRGPG LGPALRTVAT AARTLALSLD VPIVGVNHCI GHIEIGRLTT 

       130        140        150        160        170        180 
GASDPVSLYV SGGNTQVIAF NEGRYRVFGE TLDIAVGNML DQFARESGLG HPGGPVIEQL 

       190        200        210        220        230        240 
ALKASEYIEL PYSVKGMDIS FSGLLTAALR KMEAGASLED LAYSIQETAF SMLVEVTERA 

       250        260        270        280        290        300 
LAYTEKNQVL LCGGVAVNRR LRDMLREMCQ EHHVEFHMPP PEYCGDNGAM IAWLGQLVYK 

       310        320        330        340        350        360 
YRGPDALEDT TVVQRYRTDE VDVPWMRESE AGIELPPDIL AKGAEANIYR GQWIGRPCII 

       370        380        390        400        410        420 
KERISKGYRI PEIDQKLRSS RTRREARLIN QAKGAGVHTP VLFDVDPDGG VMVMEEVRGT 

       430        440        450        460        470        480 
PFRDAVEDTE LCSRIGEAIG RLHHAGIIHG DLTGSNIIIR GGDVVFIDFG LGRFSDEIED 

       490        500        510        520        530 
MGVDLLVLKK SLESTHYALA GECFSRVLEG YGKIIDADIQ SKIREIESRG RYTD 

« Hide

References

[1]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000666 Genomic DNA. Translation: AAB85902.1.
PIRH69056.
RefSeqNP_276541.1. NC_000916.1.

3D structure databases

ProteinModelPortalO27476.
SMRO27476. Positions 1-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING187420.MTH1425.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB85902; AAB85902; MTH_1425.
GeneID1471142.
KEGGmth:MTH1425.

Phylogenomic databases

eggNOGCOG0533.
KOK15904.
OMARDNAGMI.
ProtClustDBPRK09605.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-1427-MONOMER.

Family and domain databases

HAMAPMF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERPTHR11735:SF6. PTHR11735:SF6. 1 hit.
PfamPF00814. Peptidase_M22. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. SSF56112. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS01016. GLYCOPROTEASE. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_METTH
AccessionPrimary (citable) accession number: O27476
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families