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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

MTH_1425

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108IronUniRule annotation1
Metal bindingi112IronUniRule annotation1
Metal bindingi129IronUniRule annotation1
Binding sitei161Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei174Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation1
Binding sitei178Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei258Threonylcarbamoyl-AMPUniRule annotation1
Metal bindingi286IronUniRule annotation1
Binding sitei361ATPUniRule annotation1
Active sitei451Proton acceptor; for kinase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi340 – 348ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:MTH_1425
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000969801 – 534Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinAdd BLAST534

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1425.

Structurei

3D structure databases

ProteinModelPortaliO27476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini335 – 534Protein kinaseUniRule annotationAdd BLAST200

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 325Kae1Add BLAST325
Regioni129 – 133Threonylcarbamoyl-AMP bindingUniRule annotation5

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
arCOG01185. Archaea.
COG0533. LUCA.
COG3642. LUCA.
KOiK15904.
OMAiGETMDTG.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
MF_01447. Kae1_Bud32_arch. 1 hit.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCLGIEGTA EKTGVGIVDE AGNVLSLRGK PLIPEKGGIH PREAAEHHAK
60 70 80 90 100
WIPRLIAEAC RDAGVELGEI GLISFSRGPG LGPALRTVAT AARTLALSLD
110 120 130 140 150
VPIVGVNHCI GHIEIGRLTT GASDPVSLYV SGGNTQVIAF NEGRYRVFGE
160 170 180 190 200
TLDIAVGNML DQFARESGLG HPGGPVIEQL ALKASEYIEL PYSVKGMDIS
210 220 230 240 250
FSGLLTAALR KMEAGASLED LAYSIQETAF SMLVEVTERA LAYTEKNQVL
260 270 280 290 300
LCGGVAVNRR LRDMLREMCQ EHHVEFHMPP PEYCGDNGAM IAWLGQLVYK
310 320 330 340 350
YRGPDALEDT TVVQRYRTDE VDVPWMRESE AGIELPPDIL AKGAEANIYR
360 370 380 390 400
GQWIGRPCII KERISKGYRI PEIDQKLRSS RTRREARLIN QAKGAGVHTP
410 420 430 440 450
VLFDVDPDGG VMVMEEVRGT PFRDAVEDTE LCSRIGEAIG RLHHAGIIHG
460 470 480 490 500
DLTGSNIIIR GGDVVFIDFG LGRFSDEIED MGVDLLVLKK SLESTHYALA
510 520 530
GECFSRVLEG YGKIIDADIQ SKIREIESRG RYTD
Length:534
Mass (Da):58,298
Last modified:January 1, 1998 - v1
Checksum:i5DD30415047EE087
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85902.1.
PIRiH69056.
RefSeqiWP_010877037.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85902; AAB85902; MTH_1425.
GeneIDi1471142.
KEGGimth:MTH_1425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85902.1.
PIRiH69056.
RefSeqiWP_010877037.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85902; AAB85902; MTH_1425.
GeneIDi1471142.
KEGGimth:MTH_1425.

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
arCOG01185. Archaea.
COG0533. LUCA.
COG3642. LUCA.
KOiK15904.
OMAiGETMDTG.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
MF_01447. Kae1_Bud32_arch. 1 hit.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAE1B_METTH
AccessioniPrimary (citable) accession number: O27476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.