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O27476

- KAE1B_METTH

UniProt

O27476 - KAE1B_METTH

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Protein
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Gene
MTH_1425
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081Iron By similarity
Metal bindingi112 – 1121Iron By similarity
Metal bindingi129 – 1291Iron By similarity
Binding sitei161 – 1611Threonylcarbamoyl-AMP By similarity
Binding sitei174 – 1741Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding sitei178 – 1781Threonylcarbamoyl-AMP By similarity
Binding sitei258 – 2581Threonylcarbamoyl-AMP By similarity
Metal bindingi286 – 2861Iron By similarity
Binding sitei361 – 3611ATP By similarity
Active sitei451 – 4511Proton acceptor; for kinase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi340 – 3489ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
  5. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  6. protein tyrosine kinase activity Source: InterPro
  7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  8. zinc ion binding Source: InterPro

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
Serine/threonine-protein kinase Bud32 (EC:2.7.11.1)
Gene namesi
Ordered Locus Names:MTH_1425
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
PRO_0000096980Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Protein-protein interaction databases

STRINGi187420.MTH1425.

Structurei

3D structure databases

ProteinModelPortaliO27476.
SMRiO27476. Positions 1-325.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini335 – 534200Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 325325Kae1UniRule annotation
Add
BLAST
Regioni129 – 1335Threonylcarbamoyl-AMP binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Phylogenomic databases

eggNOGiCOG0533.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27476-1 [UniParc]FASTAAdd to Basket

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MLCLGIEGTA EKTGVGIVDE AGNVLSLRGK PLIPEKGGIH PREAAEHHAK    50
WIPRLIAEAC RDAGVELGEI GLISFSRGPG LGPALRTVAT AARTLALSLD 100
VPIVGVNHCI GHIEIGRLTT GASDPVSLYV SGGNTQVIAF NEGRYRVFGE 150
TLDIAVGNML DQFARESGLG HPGGPVIEQL ALKASEYIEL PYSVKGMDIS 200
FSGLLTAALR KMEAGASLED LAYSIQETAF SMLVEVTERA LAYTEKNQVL 250
LCGGVAVNRR LRDMLREMCQ EHHVEFHMPP PEYCGDNGAM IAWLGQLVYK 300
YRGPDALEDT TVVQRYRTDE VDVPWMRESE AGIELPPDIL AKGAEANIYR 350
GQWIGRPCII KERISKGYRI PEIDQKLRSS RTRREARLIN QAKGAGVHTP 400
VLFDVDPDGG VMVMEEVRGT PFRDAVEDTE LCSRIGEAIG RLHHAGIIHG 450
DLTGSNIIIR GGDVVFIDFG LGRFSDEIED MGVDLLVLKK SLESTHYALA 500
GECFSRVLEG YGKIIDADIQ SKIREIESRG RYTD 534
Length:534
Mass (Da):58,298
Last modified:January 1, 1998 - v1
Checksum:i5DD30415047EE087
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85902.1.
PIRiH69056.
RefSeqiNP_276541.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85902; AAB85902; MTH_1425.
GeneIDi1471142.
KEGGimth:MTH1425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85902.1 .
PIRi H69056.
RefSeqi NP_276541.1. NC_000916.1.

3D structure databases

ProteinModelPortali O27476.
SMRi O27476. Positions 1-325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH1425.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85902 ; AAB85902 ; MTH_1425 .
GeneIDi 1471142.
KEGGi mth:MTH1425.

Phylogenomic databases

eggNOGi COG0533.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci MTHE187420:GJNM-1427-MONOMER.

Family and domain databases

HAMAPi MF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR000719. Prot_kinase_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiKAE1B_METTH
AccessioniPrimary (citable) accession number: O27476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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