O27434 (HDRA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CoB--CoM heterodisulfide reductase iron-sulfur subunit A
EC=1.8.98.1

Gene names

Name:	hdrA
Ordered Locus Names:	MTH_1381

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). May act as the catalytic subunit By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Cofactor	Binds 4 4Fe-4S clusters per subunit By similarity. FAD By similarity.
Pathway	Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase is composed of three subunits; hdrA, hdrB and hdrC. It forms a complex with the F420-non-reducing hydrogenase (Mvh), which provides the reducing equivalents to the heterodisulfide reductase By similarity.
Sequence similarities	Belongs to the hdrA family. Contains 4 4Fe-4S ferredoxin-type domains.
Sequence caution	The sequence AAB85858.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	4Fe-4S FAD Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 659	659	CoB--CoM heterodisulfide reductase iron-sulfur subunit A		PRO_0000150061
Regions
	Domain	245 – 275	31	4Fe-4S ferredoxin-type 1
	Domain	293 – 322	30	4Fe-4S ferredoxin-type 2
	Domain	582 – 611	30	4Fe-4S ferredoxin-type 3
	Domain	612 – 641	30	4Fe-4S ferredoxin-type 4
	Nucleotide binding	159 – 182	24	FAD Potential
Sites
	Metal binding	255	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	258	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	261	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	265	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	302	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	305	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	308	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	312	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	592	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	595	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	598	1	Iron-sulfur 3 (4Fe-4S) Potential
	Metal binding	602	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	621	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	624	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	627	1	Iron-sulfur 4 (4Fe-4S) Potential
	Metal binding	631	1	Iron-sulfur 3 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O27434 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: ED517077EC5B6CBD	FASTA	659	72,392
	10 20 30 40 50 60 MAEEKKEETM EEPRIGVYVC HCGVNIGGVV DIEAVRDYAA KLPNVVVSKD YKYYCSDPGQ 70 80 90 100 110 120 LEIQKDIKEL GLNRVVVAAC SPRLHEPTFR RCVEEAGLNQ FLFEFANLRE QDSWVHMDDP 130 140 150 160 170 180 EGATEKAKDL VRMAVAKARL LEPLEASKVS VDDKALVIGG GVAGIQTALD LADMGFKTYM 190 200 210 220 230 240 VEKRPSISGR MGQLDKTFPT LDCSMCILAP KMVDVGKHDN IELITYAEVK EVDGYIGNFK 250 260 270 280 290 300 VKIEKKPRYI DEDLCTGCGS CVEVCPIEMP NYFDEGIGMT KAVYIPFPQA VPLCATIDKD 310 320 330 340 350 360 YCIECMLCDE ICERGAVKHD QEPEEIEIEV GTIIVATGYD AYDPTEKLEY GYGRHTNVIT 370 380 390 400 410 420 GLELERMINA SGPTDGKVIK PSDGEKPKRV AFIHCVGSRD EQIGKPYCSR VCCMYIMKNA 430 440 450 460 470 480 QLIKDKMPDT EVTLYYMDIR AFGKGFEEFY KRSQEKYGIK FIRGRPAEIL ENPDLTLTVR 490 500 510 520 530 540 SEDTLLGKVT EYDYDMVVLG VGLVPPEGSE KLRQTIGLSK SADGFLMEAH PKLRPVDTLT 550 560 570 580 590 600 DGVYLAGVAQ GPKDIPDAVA QASGAAARAA IPMVKGEVEI EPIVAVTDSD VCGGCEVCIE 610 620 630 640 650 LCPFGAISIE EGHANVNVAL CKGCGTCVAA CPSGAMDQQH FRTEQIMAQI EAALNEQAK

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85858.1. Different initiation.
PIR	G69050.
RefSeq	NP_276497.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27434.
ModBase	Search...
Protein-protein interaction databases
IntAct	O27434. 2 interactions.
STRING	O27434.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471098.
GenomeReviews	Gene locus MTH_1381 in contig AE000666_GR.
KEGG	mth:MTH1381.
NMPDR	fig|187420.1.peg.1352.
Phylogenomic databases
eggNOG	arNOG04853.
HOGENOM	HBG452010.
OMA	TNDCSIC.
ProtClustDB	CLSK876667.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1381-MONOMER.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. [Graphical view]
KO	K03388.
Pfam	PF00037. Fer4. 3 hits. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PROSITE	PS00198. 4FE4S_FER_1. 4 hits. PS51379. 4FE4S_FER_2. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HDRA_METTH

Accession

Primary (citable) accession number: O27434

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	November 16, 2011
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program