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Protein

H(2):CoB-CoM heterodisulfide,ferredoxin reductase subunit A

Gene

hdrA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).By similarity

Catalytic activityi

2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ = 2 H2 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB.By similarity

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 4 [4Fe-4S] clusters per subunit.PROSITE-ProRule annotation
  • FADBy similarity

Pathwayi: coenzyme M-coenzyme B heterodisulfide reduction

This protein is involved in step 1 of the subpathway that synthesizes coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. H(2):CoB-CoM heterodisulfide,ferredoxin reductase subunit C (hdrC), H(2):CoB-CoM heterodisulfide,ferredoxin reductase subunit B (hdrB), H(2):CoB-CoM heterodisulfide,ferredoxin reductase subunit A (hdrA)
This subpathway is part of the pathway coenzyme M-coenzyme B heterodisulfide reduction, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide, the pathway coenzyme M-coenzyme B heterodisulfide reduction and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi255Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi258Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi261Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi265Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi302Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi305Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi308Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi312Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi592Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi595Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi598Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi602Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi621Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi624Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi627Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi631Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi159 – 182FADSequence analysisAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processMethanogenesis
Ligand4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HDRAMAUTO-MONOMER
UniPathwayiUPA00647; UER00700

Names & Taxonomyi

Protein namesi
Recommended name:
H(2):CoB-CoM heterodisulfide,ferredoxin reductase subunit ABy similarity (EC:1.8.98.5By similarity)
Alternative name(s):
CoB--CoM heterodisulfide reductase iron-sulfur subunit ABy similarity
Gene namesi
Name:hdrA
Ordered Locus Names:MTH_1381
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001500611 – 659H(2):CoB-CoM heterodisulfide,ferredoxin reductase subunit AAdd BLAST659

Interactioni

Subunit structurei

The heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC. It forms a complex with the F420-non-reducing hydrogenase (Mvh), which provides the reducing equivalents to the heterodisulfide reductase.By similarity

Protein-protein interaction databases

IntActiO27434, 2 interactors
STRINGi187420.MTH1381

Structurei

3D structure databases

ProteinModelPortaliO27434
SMRiO27434
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini245 – 2754Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST31
Domaini293 – 3224Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30
Domaini582 – 6114Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30
Domaini612 – 6414Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Belongs to the HdrA family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG02235 Archaea
COG1148 LUCA
KOiK03388
OMAiTAKHAML
OrthoDBiPOG093Z04QZ

Family and domain databases

Gene3Di3.50.50.60, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
PfamiView protein in Pfam
PF00037 Fer4, 1 hit
PF13237 Fer4_10, 1 hit
PF07992 Pyr_redox_2, 2 hits
SUPFAMiSSF51905 SSF51905, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 4 hits
PS51379 4FE4S_FER_2, 4 hits

Sequencei

Sequence statusi: Complete.

O27434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEKKEETM EEPRIGVYVC HCGVNIGGVV DIEAVRDYAA KLPNVVVSKD
60 70 80 90 100
YKYYCSDPGQ LEIQKDIKEL GLNRVVVAAC SPRLHEPTFR RCVEEAGLNQ
110 120 130 140 150
FLFEFANLRE QDSWVHMDDP EGATEKAKDL VRMAVAKARL LEPLEASKVS
160 170 180 190 200
VDDKALVIGG GVAGIQTALD LADMGFKTYM VEKRPSISGR MGQLDKTFPT
210 220 230 240 250
LDCSMCILAP KMVDVGKHDN IELITYAEVK EVDGYIGNFK VKIEKKPRYI
260 270 280 290 300
DEDLCTGCGS CVEVCPIEMP NYFDEGIGMT KAVYIPFPQA VPLCATIDKD
310 320 330 340 350
YCIECMLCDE ICERGAVKHD QEPEEIEIEV GTIIVATGYD AYDPTEKLEY
360 370 380 390 400
GYGRHTNVIT GLELERMINA SGPTDGKVIK PSDGEKPKRV AFIHCVGSRD
410 420 430 440 450
EQIGKPYCSR VCCMYIMKNA QLIKDKMPDT EVTLYYMDIR AFGKGFEEFY
460 470 480 490 500
KRSQEKYGIK FIRGRPAEIL ENPDLTLTVR SEDTLLGKVT EYDYDMVVLG
510 520 530 540 550
VGLVPPEGSE KLRQTIGLSK SADGFLMEAH PKLRPVDTLT DGVYLAGVAQ
560 570 580 590 600
GPKDIPDAVA QASGAAARAA IPMVKGEVEI EPIVAVTDSD VCGGCEVCIE
610 620 630 640 650
LCPFGAISIE EGHANVNVAL CKGCGTCVAA CPSGAMDQQH FRTEQIMAQI

EAALNEQAK
Length:659
Mass (Da):72,392
Last modified:January 16, 2004 - v2
Checksum:iED517077EC5B6CBD
GO

Sequence cautioni

The sequence AAB85858 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB85858.1 Different initiation.
PIRiG69050
RefSeqiWP_048061038.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaiAAB85858; AAB85858; MTH_1381
GeneIDi24854496
KEGGimth:MTH_1381
PATRICifig|187420.15.peg.1346

Similar proteinsi

Entry informationi

Entry nameiHDRA_METTH
AccessioniPrimary (citable) accession number: O27434
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: April 25, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health