O27428 (SYI_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	MTH_1375

Organism

Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanobacterium thermoformicicum

Protein attributes

Sequence length	1044 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003
Cofactor	Zinc By similarity. HAMAP-Rule MF_02003
Subunit structure	Monomer By similarity. HAMAP-Rule MF_02003
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_02003.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1044	1043	Isoleucine--tRNA ligase HAMAP-Rule MF_02003		PRO_0000098578
Regions
	Motif	49 – 59	11	"HIGH" region HAMAP-Rule MF_02003
	Motif	591 – 595	5	"KMSKS" region HAMAP-Rule MF_02003
Sites
	Binding site	594	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27428 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: BDE882817791051B	FASTA	1,044	120,980
	10 20 30 40 50 60 MPIQEAEKSY RPHRIEEKVQ SFWEERDIYE RVKELREDRP RYSFLDGPPY CSGRIHLGTA 70 80 90 100 110 120 WNKIMKDTYL RFKSMKGFNV RRQPGWDTHG LPIEHKVEGL LGVRSKKDIE DKIGIEEFVN 130 140 150 160 170 180 KCREFAVENK AVMTGQFQRL GVWMDWDDPY VTFDPAYMES CWWTLKRAHE KDLLVRDLRV 190 200 210 220 230 240 ITWCPRCETA LALAEIDYHD KEDPSIYVKF PVSGDTHILV WTTTPWTLPA NMAVAVHPDF 250 260 270 280 290 300 EYAYARLDGE TYIMAEALVE KVLGEEAEIL KRVKGTELEG LTYRHPLDDE VPLHREIEHR 310 320 330 340 350 360 VILGDHVTLT EGTGCVHTAP GHGPEDFEIG KKYGLEVICP VDEAGIFTEE AGKYSGRFVK 370 380 390 400 410 420 DADEDIIADL RSKGLLLKAG TISHRYGFCW RCKTPIIYLA TEQWFLKITE IKDKMLRELD 430 440 450 460 470 480 RVQWVPSWAG ESRFRNWIEN ARDWTISRQR YWGIPIPIWI CEECDSIHVV GSIDELRELA 490 500 510 520 530 540 VEGELEGDFI HRPHVDRIVL ECGECGGRMK RTPDVLDVWI DSGVAGWAAL HYPSETELFR 550 560 570 580 590 600 EWFPYDFITE GHDQTRGWFY SQLGCGVIAL DEVPYRRVLM HGFTLDEEGR KMSKSLGNVV 610 620 630 640 650 660 EPEDVIEKYG ADVLRFYLLW ENKPWEDLKF VWDELRNVNK MFNILWNVYV FATTYMSLDR 670 680 690 700 710 720 FQPGDHSAED LSFRDEDRWI LSRINSVALK VTEAIENLHF HRATREIHDF IVEDLSRWYI 730 740 750 760 770 780 RLIRSRTWIE RDDPDKLAAY HTLYTVLKTL IVTLSPMAPH VCEDIYQNLV RGAEPDSPES 790 800 810 820 830 840 IHMLDWILDE GAVDSQLEAD MDIVREIIEA CARARDTARY KLRWPVREMV VVSEDEGVLK 850 860 870 880 890 900 AAESLKNVIA EQANAKSIKT STEFPDMKII ARPNPATLGP RLRQDIPLVM RELEGADGSA 910 920 930 940 950 960 VKAALDSDGE FTVETDGKKF KLTSEDIVFE TELPENIVSA QFDGGSVFID TELTPEIMSE 970 980 990 1000 1010 1020 AMARELVRRI QDMRKDLDLD VEASIEVSVK CSEEFRELTE PQREFIENEV RASTLSFDYS 1030 1040 ELEYTKEWKI SDENLIISIK PAKV

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85852.1.
PIR	H69049.
RefSeq	NP_276491.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27428.
ModBase	Search...
Protein-protein interaction databases
STRING	187420.MTH1375.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB85852; AAB85852; MTH_1375.
GeneID	1471092.
KEGG	mth:MTH1375.
Phylogenomic databases
eggNOG	COG0060.
KO	K01870.
OMA	WFYSQLG.
ProtClustDB	PRK06039.
Enzyme and pathway databases
BioCyc	MTHE187420:GJNM-1377-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02003. Ile_tRNA_synth_type2.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_METTH

Accession

Primary (citable) accession number: O27428

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program