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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMTHE187420:G1G16-1332-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MTH_1375
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000985782 – 1044Isoleucine--tRNA ligaseAdd BLAST1043

Proteomic databases

PRIDEiO27428

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1375

Structurei

3D structure databases

ProteinModelPortaliO27428
SMRiO27428
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 59"HIGH" regionAdd BLAST11
Motifi591 – 595"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O27428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIQEAEKSY RPHRIEEKVQ SFWEERDIYE RVKELREDRP RYSFLDGPPY
60 70 80 90 100
CSGRIHLGTA WNKIMKDTYL RFKSMKGFNV RRQPGWDTHG LPIEHKVEGL
110 120 130 140 150
LGVRSKKDIE DKIGIEEFVN KCREFAVENK AVMTGQFQRL GVWMDWDDPY
160 170 180 190 200
VTFDPAYMES CWWTLKRAHE KDLLVRDLRV ITWCPRCETA LALAEIDYHD
210 220 230 240 250
KEDPSIYVKF PVSGDTHILV WTTTPWTLPA NMAVAVHPDF EYAYARLDGE
260 270 280 290 300
TYIMAEALVE KVLGEEAEIL KRVKGTELEG LTYRHPLDDE VPLHREIEHR
310 320 330 340 350
VILGDHVTLT EGTGCVHTAP GHGPEDFEIG KKYGLEVICP VDEAGIFTEE
360 370 380 390 400
AGKYSGRFVK DADEDIIADL RSKGLLLKAG TISHRYGFCW RCKTPIIYLA
410 420 430 440 450
TEQWFLKITE IKDKMLRELD RVQWVPSWAG ESRFRNWIEN ARDWTISRQR
460 470 480 490 500
YWGIPIPIWI CEECDSIHVV GSIDELRELA VEGELEGDFI HRPHVDRIVL
510 520 530 540 550
ECGECGGRMK RTPDVLDVWI DSGVAGWAAL HYPSETELFR EWFPYDFITE
560 570 580 590 600
GHDQTRGWFY SQLGCGVIAL DEVPYRRVLM HGFTLDEEGR KMSKSLGNVV
610 620 630 640 650
EPEDVIEKYG ADVLRFYLLW ENKPWEDLKF VWDELRNVNK MFNILWNVYV
660 670 680 690 700
FATTYMSLDR FQPGDHSAED LSFRDEDRWI LSRINSVALK VTEAIENLHF
710 720 730 740 750
HRATREIHDF IVEDLSRWYI RLIRSRTWIE RDDPDKLAAY HTLYTVLKTL
760 770 780 790 800
IVTLSPMAPH VCEDIYQNLV RGAEPDSPES IHMLDWILDE GAVDSQLEAD
810 820 830 840 850
MDIVREIIEA CARARDTARY KLRWPVREMV VVSEDEGVLK AAESLKNVIA
860 870 880 890 900
EQANAKSIKT STEFPDMKII ARPNPATLGP RLRQDIPLVM RELEGADGSA
910 920 930 940 950
VKAALDSDGE FTVETDGKKF KLTSEDIVFE TELPENIVSA QFDGGSVFID
960 970 980 990 1000
TELTPEIMSE AMARELVRRI QDMRKDLDLD VEASIEVSVK CSEEFRELTE
1010 1020 1030 1040
PQREFIENEV RASTLSFDYS ELEYTKEWKI SDENLIISIK PAKV
Length:1,044
Mass (Da):120,980
Last modified:January 23, 2007 - v3
Checksum:iBDE882817791051B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB85852.1
PIRiH69049
RefSeqiWP_010876987.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaiAAB85852; AAB85852; MTH_1375
GeneIDi1471092
KEGGimth:MTH_1375
PATRICifig|187420.15.peg.1340

Similar proteinsi

Entry informationi

Entry nameiSYI_METTH
AccessioniPrimary (citable) accession number: O27428
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 122 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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