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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1377-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MTH_1375
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 10441043Isoleucine--tRNA ligasePRO_0000098578Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1375.

Structurei

3D structure databases

ProteinModelPortaliO27428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiEGPPTTN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O27428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIQEAEKSY RPHRIEEKVQ SFWEERDIYE RVKELREDRP RYSFLDGPPY
60 70 80 90 100
CSGRIHLGTA WNKIMKDTYL RFKSMKGFNV RRQPGWDTHG LPIEHKVEGL
110 120 130 140 150
LGVRSKKDIE DKIGIEEFVN KCREFAVENK AVMTGQFQRL GVWMDWDDPY
160 170 180 190 200
VTFDPAYMES CWWTLKRAHE KDLLVRDLRV ITWCPRCETA LALAEIDYHD
210 220 230 240 250
KEDPSIYVKF PVSGDTHILV WTTTPWTLPA NMAVAVHPDF EYAYARLDGE
260 270 280 290 300
TYIMAEALVE KVLGEEAEIL KRVKGTELEG LTYRHPLDDE VPLHREIEHR
310 320 330 340 350
VILGDHVTLT EGTGCVHTAP GHGPEDFEIG KKYGLEVICP VDEAGIFTEE
360 370 380 390 400
AGKYSGRFVK DADEDIIADL RSKGLLLKAG TISHRYGFCW RCKTPIIYLA
410 420 430 440 450
TEQWFLKITE IKDKMLRELD RVQWVPSWAG ESRFRNWIEN ARDWTISRQR
460 470 480 490 500
YWGIPIPIWI CEECDSIHVV GSIDELRELA VEGELEGDFI HRPHVDRIVL
510 520 530 540 550
ECGECGGRMK RTPDVLDVWI DSGVAGWAAL HYPSETELFR EWFPYDFITE
560 570 580 590 600
GHDQTRGWFY SQLGCGVIAL DEVPYRRVLM HGFTLDEEGR KMSKSLGNVV
610 620 630 640 650
EPEDVIEKYG ADVLRFYLLW ENKPWEDLKF VWDELRNVNK MFNILWNVYV
660 670 680 690 700
FATTYMSLDR FQPGDHSAED LSFRDEDRWI LSRINSVALK VTEAIENLHF
710 720 730 740 750
HRATREIHDF IVEDLSRWYI RLIRSRTWIE RDDPDKLAAY HTLYTVLKTL
760 770 780 790 800
IVTLSPMAPH VCEDIYQNLV RGAEPDSPES IHMLDWILDE GAVDSQLEAD
810 820 830 840 850
MDIVREIIEA CARARDTARY KLRWPVREMV VVSEDEGVLK AAESLKNVIA
860 870 880 890 900
EQANAKSIKT STEFPDMKII ARPNPATLGP RLRQDIPLVM RELEGADGSA
910 920 930 940 950
VKAALDSDGE FTVETDGKKF KLTSEDIVFE TELPENIVSA QFDGGSVFID
960 970 980 990 1000
TELTPEIMSE AMARELVRRI QDMRKDLDLD VEASIEVSVK CSEEFRELTE
1010 1020 1030 1040
PQREFIENEV RASTLSFDYS ELEYTKEWKI SDENLIISIK PAKV
Length:1,044
Mass (Da):120,980
Last modified:January 23, 2007 - v3
Checksum:iBDE882817791051B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85852.1.
PIRiH69049.
RefSeqiNP_276491.1. NC_000916.1.
WP_010876987.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85852; AAB85852; MTH_1375.
GeneIDi1471092.
KEGGimth:MTH1375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85852.1.
PIRiH69049.
RefSeqiNP_276491.1. NC_000916.1.
WP_010876987.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1375.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85852; AAB85852; MTH_1375.
GeneIDi1471092.
KEGGimth:MTH1375.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiEGPPTTN.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1377-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiSYI_METTH
AccessioniPrimary (citable) accession number: O27428
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.