O27427 (PURL_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	MTH_1374

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100516
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O27427 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7631762D21013D9C	FASTA	714	76,823
	10 20 30 40 50 60 MVLTDSEMEF IRKELGRDPN PLEYGMLDVM FSEHCSYKSS RPVLGLFPTE GEDVIIGPGD 70 80 90 100 110 120 DAGVVEITDE LALVIGIESH NHPSAIEPYG GAGTGIGGIL RDIISMGAMP VALLDSLRFG 130 140 150 160 170 180 YLEDQKSRYL FEHVVRGISD YGNRVGVPTV AGEVEFDENF QLNPLVNVMC AGLVPKNDIK 190 200 210 220 230 240 RGIAPRPGDV FLLMGGRTGR DGIHGVTFAS EELTSSSELE DRPAVQVGDP FTKKMVMEAS 250 260 270 280 290 300 FEIMDEIEVS GVKDLGGGGL TCCISELVAK CDNGARVSLE AIPLREEGMT PYEIMLSESQ 310 320 330 340 350 360 ERMIFVMAPD DVEAAMEICR KYELPAAVIG EVTDTGRMIV ESEGEVIADL PAKLLADPPV 370 380 390 400 410 420 VEREARKPQL PEGQVEVKHP PLKDTLLKLI SSPNIASKRW VYRQYDHEVQ IRTVVKPGDD 430 440 450 460 470 480 AAVLRVDGTT GVALTVDCNS IHTKLDPYGG GAASVGEAIR NVVSMGAWPV CIVDCLNFGN 490 500 510 520 530 540 PEKPEVFWQF RECVRGMADM AETFSTPVIS GNVSFYNETE GVTVNPSPVV GVAGKLSLDS 550 560 570 580 590 600 IKTLDFKDEG EEIVVIGETG PELGASEYLR TVHGIVDGKP PETDLKAEFE AAKAVKEIID 610 620 630 640 650 660 GSGNKVTAVH DCSAGGIAVA VAEMAIKSGI GARIDPMKIP GRFRNIHEAL FSESNGRYIM 670 680 690 700 710 TVRGSARDIL GGLDVPWAVI GTTGGRTLEF GDVALDISEL DDAYHGVIEA YMST

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85851.1.
PIR	G69049.
RefSeq	NP_276490.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27427.
ModBase	Search...
Protein-protein interaction databases
STRING	O27427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471091.
GenomeReviews	Gene locus MTH_1374 in contig AE000666_GR.
KEGG	mth:MTH1374.
NMPDR	fig|187420.1.peg.1345.
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	O27427.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1374-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METTH

Accession

Primary (citable) accession number: O27427

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program