O27397 (OGG1_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable N-glycosylase/DNA lyase

Including the following 2 domains:

1. 8-oxoguanine DNA glycosylase
   EC=3.2.2.-
2. DNA-(apurinic or apyrimidinic site) lyase
   Short name=AP lyase
   EC=4.2.99.18

Gene names

Ordered Locus Names:

MTH_1342

Organism

Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanobacterium thermoformicicum

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion By similarity.
Catalytic activity	The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Sequence similarities	Belongs to the type-1 OGG1 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Molecular function	Glycosidase Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: InterPro nucleotide-excision repair Inferred from electronic annotation. Source: InterPro
Molecular_function	damaged DNA binding Inferred from electronic annotation. Source: InterPro oxidized purine nucleobase lesion DNA N-glycosylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Probable N-glycosylase/DNA lyase		PRO_0000058596
Sites
	Active site	235	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27397 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D33B4FF497BC5A1C	FASTA	312	36,052
	10 20 30 40 50 60 MRIPVGDFDL EMTQRSGQTS QPPWREVEGA FRELLIIEGV PCPVEVRNEA GVLRVRPYVD 70 80 90 100 110 120 VPQKTLREKI EYIFDLKFDI EDFYTFLEDK NLSYTLDSSR GLRLFLAKDP FECVISSIAS 130 140 150 160 170 180 ANCSVVRWTR SIEDIRRLWG QANTFNGETF HTFPSPHVLT GVAEGSLEDL QRAEDNLPSD 190 200 210 220 230 240 FSFNDLRSCG VGYRAPYIRE TSRILAEEMD IRRIDGMDYD DARELLLELS GVGPKVADCI 250 260 270 280 290 300 LLYGFRKTEA FPVDVWIRRI MNHIHPGRNF NDRSMVEFAR REYGEMADYV QLYLFNHARR 310 SGLLDRLRQG TG

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85820.1.
PIR	E69045.
RefSeq	NP_276459.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27397.
ModBase	Search...
Protein-protein interaction databases
STRING	187420.MTH1342.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB85820; AAB85820; MTH_1342.
GeneID	1471059.
KEGG	mth:MTH1342.
Phylogenomic databases
eggNOG	COG0122.
KO	K03660.
OMA	DCILLYG.
ProtClustDB	CLSK798145.
Enzyme and pathway databases
BioCyc	MTHE187420:GJNM-1344-MONOMER.
Family and domain databases
Gene3D	1.10.1670.10. 1 hit. 1.10.340.30. 1 hit.
InterPro	IPR011257. DNA_glycosylase. IPR003265. HhH-GPD_domain. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR023170. HTH_base_excis_C. IPR004577. Ogg. IPR012904. OGG_N. [Graphical view]
Pfam	PF00730. HhH-GPD. 1 hit. PF07934. OGG_N. 1 hit. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. SM00278. HhH1. 1 hit. [Graphical view]
SUPFAM	SSF48150. DNA_glycsylse. 1 hit.
TIGRFAMs	TIGR00588. ogg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

OGG1_METTH

Accession

Primary (citable) accession number: O27397

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program