SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O27389

- DAPF_METTH

UniProt

O27389 - DAPF_METTH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Diaminopimelate epimerase
Gene
dapF, MTH_1334
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121Substrate By similarity
Binding sitei48 – 481Substrate By similarity
Binding sitei67 – 671Substrate By similarity
Active sitei76 – 761Proton donor/acceptor By similarity
Binding sitei165 – 1651Substrate By similarity
Sitei167 – 1671Important for catalytic activity By similarity
Binding sitei198 – 1981Substrate By similarity
Sitei216 – 2161Important for catalytic activity By similarity
Active sitei225 – 2251Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1336-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:MTH_1334
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Diaminopimelate epimeraseUniRule annotation
PRO_0000149888Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi76 ↔ 225UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH1334.

Structurei

3D structure databases

ProteinModelPortaliO27389.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 102Substrate binding By similarity
Regioni76 – 783Substrate binding By similarity
Regioni216 – 2172Substrate binding By similarity
Regioni226 – 2272Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
KOiK01778.
OMAiLIVEPPY.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27389-1 [UniParc]FASTAAdd to Basket

« Hide

MILFSKMHGL GNDYVVIDES AQECIPEDKK PEFVREVCTR GFSVGADGVI    50
FVQPASGEGD IRFRIFNADG SEAEMCGNGI RCFSKFVYDN AIVRKRRLEV 100
ETLAGIKTVE LEVEDGAVVS SRVDMGTATF KTDQIPMDVE EYEFIDRFLP 150
VEGEDIKLTA LSVGNPHAII FVDDAEGVDL DRLGPAIENH PLFPERINVH 200
FVEVVSPSEI IMVTWERGAG PTMACGTGAT ASVIAGVKLE KLDDSVLVHL 250
PGGELKIDVY QDGTELGAYM EGDAVMVFDG ILLRDP 286
Length:286
Mass (Da):31,220
Last modified:May 30, 2000 - v2
Checksum:i374300C9D7FBDDE7
GO

Sequence cautioni

The sequence AAB85812.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85812.1. Different initiation.
PIRiD69044.
RefSeqiNP_276451.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85812; AAB85812; MTH_1334.
GeneIDi1471051.
KEGGimth:MTH1334.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85812.1 . Different initiation.
PIRi D69044.
RefSeqi NP_276451.1. NC_000916.1.

3D structure databases

ProteinModelPortali O27389.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH1334.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85812 ; AAB85812 ; MTH_1334 .
GeneIDi 1471051.
KEGGi mth:MTH1334.

Phylogenomic databases

eggNOGi COG0253.
KOi K01778.
OMAi LIVEPPY.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci MTHE187420:GJNM-1336-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiDAPF_METTH
AccessioniPrimary (citable) accession number: O27389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: June 11, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi