O27369 (RPOM_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit M
EC=2.7.7.6

Gene names

Name:	rpoM
Ordered Locus Names:	MTH_1314

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. Contains 1 TFIIS-type zinc finger.
Sequence caution	The sequence AAB85792.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 104	104	DNA-directed RNA polymerase subunit M		PRO_0000121482
Regions
	Zinc finger	4 – 22	19	C4-type Potential
	Zinc finger	61 – 101	41	TFIIS-type

Sequences

	Sequence		Length	Mass (Da)
	O27369 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 36DACA10927C5CE8	FASTA	104	12,356
	10 20 30 40 50 60 MEFCPKCGAV MFPSEGKFKC QCGYEKDITD KLKDKYRVSE EVEAKETIIF TGDDVNTLPT 70 80 90 100 TRVECPKCGN MEAFWWLQQT RRADESETRF FRCTRCKHTW REYD

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85792.1. Different initiation.
PIR	F69041.
RefSeq	NP_276431.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27369.
ModBase	Search...
Protein-protein interaction databases
STRING	O27369.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471031.
GenomeReviews	Gene locus MTH_1314 in contig AE000666_GR.
KEGG	mth:MTH1314.
Phylogenomic databases
eggNOG	arNOG11320.
HOGENOM	HBG717463.
OMA	ETRFFKC.
ProtClustDB	CLSK342434.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1314-MONOMER.
Family and domain databases
InterPro	IPR019761. DNA-dir_RNA_pol-M_15_CS. IPR001529. DNA-dir_RNA_pol_M/15kDasu. IPR006288. DNA-dir_RNA_pol_suM_arc. IPR012164. DNA-dir_RNApol_C11. IPR001222. Znf_TFIIS. [Graphical view]
KO	K03057.
Pfam	PF02150. RNA_POL_M_15KD. 1 hit. PF01096. TFIIS_C. 1 hit. [Graphical view]
PIRSF	PIRSF005586. RNApol_RpoM. 1 hit.
SMART	SM00661. RPOL9. 1 hit. SM00440. ZnF_C2C2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01384. TFS_arch. 1 hit.
PROSITE	PS01030. RNA_POL_M_15KD. 1 hit. PS00466. ZF_TFIIS_1. 1 hit. PS51133. ZF_TFIIS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOM_METTH

Accession

Primary (citable) accession number: O27369

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program