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Protein

DNA polymerase sliding clamp

Gene

pcn

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication (By similarity). In conjunction with replication factor C (RFC) stimulates DNA synthesis by PolB, relieving inhbition by replication protein A (RPA).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1314-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase sliding clampUniRule annotation
Alternative name(s):
Proliferating cell nuclear antigen homologUniRule annotation
Short name:
PCNAUniRule annotation
Gene namesi
Name:pcnUniRule annotation
Ordered Locus Names:MTH_1312
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244DNA polymerase sliding clampPRO_0000149200Add
BLAST

Interactioni

Subunit structurei

Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1312.

Structurei

3D structure databases

ProteinModelPortaliO27367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
KOiK04802.
OMAiANVGMVD.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKAELNDPN ILRTSFDAIS SIVDEVQIQL SAEGLRLDAL DRSHITYVHL
60 70 80 90 100
ELKAELFDEY VCDEPERINV DTEELMKVLK RAKANDRVIL STDEGNLIIQ
110 120 130 140 150
FEGEAVRTFK IRLIDIEYET PSPPEIEYEN EFEVPFQLLK DSIADIDIFS
160 170 180 190 200
DKITFRVDED RFIASAEGEF GDAQIEYLHG ERIDKPARSI YSLDKIKEML
210 220 230 240
KADKFSETAI INLGDDMPLK LTLKMASKEG ELSFLLAPRI EAEE
Length:244
Mass (Da):27,969
Last modified:January 1, 1998 - v1
Checksum:iCCE44AAE59139CB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85790.1.
PIRiD69041.
RefSeqiWP_010876925.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85790; AAB85790; MTH_1312.
GeneIDi1471029.
KEGGimth:MTH_1312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85790.1.
PIRiD69041.
RefSeqiWP_010876925.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1312.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85790; AAB85790; MTH_1312.
GeneIDi1471029.
KEGGimth:MTH_1312.

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
KOiK04802.
OMAiANVGMVD.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1314-MONOMER.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. "Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH."
    Kelman Z., Pietrokovski S., Hurwitz J.
    J. Biol. Chem. 274:28751-28761(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiPCNA_METTH
AccessioniPrimary (citable) accession number: O27367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.