Reviewed, UniProtKB/Swiss-Prot O27355 (AMPM_METTH)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	MTH_1296

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Methionine aminopeptidase		PRO_0000148976
Sites
	Metal binding	84	1	Cobalt 1 By similarity
	Metal binding	95	1	Cobalt 1 By similarity
	Metal binding	95	1	Cobalt 2 By similarity
	Metal binding	158	1	Cobalt 2 By similarity
	Metal binding	191	1	Cobalt 2 By similarity
	Metal binding	284	1	Cobalt 1 By similarity
	Metal binding	284	1	Cobalt 2 By similarity
	Binding site	64	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27355-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: DA37006A06638167	FASTA	299	33,431
	10 20 30 40 50 60 MIKMIESYLK AGKIVSKVRR EAAGIIRDGL PIIELVNYVE DRIIEEGGRP AFPCNVSVNE 70 80 90 100 110 120 VTAHYTSPPG DDSVIGDGDL VKLDLGAHVD GFIADTAITV PVGDVDDKCH QMMDAAREAL 130 140 150 160 170 180 ENAISTIRAG VEVGEIGRVI EETIHSHGMN PVSNLTGHSM ERWILHSGLS IPNINERNTH 190 200 210 220 230 240 QLEEGDVLAI EPFATDGVGL VTDMPQTYIF RFLRERPLRL VHARRVLGKI REEYHALPFA 250 260 270 280 290 QRWLEEYFDA KRLNASMRML IQSRAIYPYH VLREKSGAMV AQWEHTVIVE EDGCTVITE

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000666 Genomic DNA. Translation: AAB85776.1.
PIR	C69039.
RefSeq	NP_276415.1.
3D structure databases
HSSP	HSSP built from PDB template 1XGS based on UniProtKB P56218.
ModBase	Search...
Protein-protein interaction databases
STRING	O27355.
Protein family/group databases
MEROPS	M24.002.
Genome annotation databases
GeneID	1471013.
GenomeReviews	Gene locus MTH_1296 in contig AE000666_GR.
KEGG	mth:MTH1296.
NMPDR	fig|187420.1.peg.1270.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O27355.
OMA	GHKIERY.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1296-MON.
BRENDA	3.4.11.18. 270.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002468. Pept_M24A_MAP2. IPR018349. Pept_M24A_MAP2_BS. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00501. met_pdase_II. 1 hit.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_METTH

Accession

Primary (citable) accession number: O27355

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)