Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionine aminopeptidase

Gene

map

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64SubstrateUniRule annotation1
Metal bindingi84Divalent metal cation 1UniRule annotation1
Metal bindingi95Divalent metal cation 1UniRule annotation1
Metal bindingi95Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi158Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Metal bindingi191Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi284Divalent metal cation 1UniRule annotation1
Metal bindingi284Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Protein family/group databases

MEROPSiM24.035

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:MTH_1296
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489761 – 299Methionine aminopeptidaseAdd BLAST299

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1296

Structurei

3D structure databases

ProteinModelPortaliO27355
SMRiO27355
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01001 Archaea
COG0024 LUCA
KOiK01265
OMAiHTVLLMP
OrthoDBiPOG093Z06FJ

Family and domain databases

CDDicd01088 MetAP2, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_01975 MetAP_2_arc, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR028595 MetAP_archaeal
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002468 Pept_M24A_MAP2
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 2 hits
TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

O27355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKMIESYLK AGKIVSKVRR EAAGIIRDGL PIIELVNYVE DRIIEEGGRP
60 70 80 90 100
AFPCNVSVNE VTAHYTSPPG DDSVIGDGDL VKLDLGAHVD GFIADTAITV
110 120 130 140 150
PVGDVDDKCH QMMDAAREAL ENAISTIRAG VEVGEIGRVI EETIHSHGMN
160 170 180 190 200
PVSNLTGHSM ERWILHSGLS IPNINERNTH QLEEGDVLAI EPFATDGVGL
210 220 230 240 250
VTDMPQTYIF RFLRERPLRL VHARRVLGKI REEYHALPFA QRWLEEYFDA
260 270 280 290
KRLNASMRML IQSRAIYPYH VLREKSGAMV AQWEHTVIVE EDGCTVITE
Length:299
Mass (Da):33,431
Last modified:January 1, 1998 - v1
Checksum:iDA37006A06638167
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA Translation: AAB85776.1
PIRiC69039

Genome annotation databases

EnsemblBacteriaiAAB85776; AAB85776; MTH_1296
KEGGimth:MTH_1296
PATRICifig|187420.15.peg.1267

Similar proteinsi

Entry informationi

Entry nameiMAP2_METTH
AccessioniPrimary (citable) accession number: O27355
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health