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O27355

- MAP2_METTH

UniProt

O27355 - MAP2_METTH

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Protein

Methionine aminopeptidase

Gene
map, MTH_1296
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val) By similarity.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641Substrate By similarity
Metal bindingi84 – 841Divalent metal cation 1 By similarity
Metal bindingi95 – 951Divalent metal cation 1 By similarity
Metal bindingi95 – 951Divalent metal cation 2; catalytic By similarity
Metal bindingi158 – 1581Divalent metal cation 2; catalytic; via tele nitrogen By similarity
Binding sitei166 – 1661Substrate By similarity
Metal bindingi191 – 1911Divalent metal cation 2; catalytic By similarity
Metal bindingi284 – 2841Divalent metal cation 1 By similarity
Metal bindingi284 – 2841Divalent metal cation 2; catalytic By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1298-MONOMER.

Protein family/group databases

MEROPSiM24.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase (EC:3.4.11.18)
Short name:
MAP
Short name:
MetAP
Alternative name(s):
Peptidase M
Gene namesi
Name:map
Ordered Locus Names:MTH_1296
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Methionine aminopeptidaseUniRule annotationPRO_0000148976Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi187420.MTH1296.

Structurei

3D structure databases

ProteinModelPortaliO27355.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0024.
KOiK01265.
OMAiNNCVAHY.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_01975. MetAP_2_arc.
InterProiIPR028595. MetAP_archaeal.
IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27355-1 [UniParc]FASTAAdd to Basket

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MIKMIESYLK AGKIVSKVRR EAAGIIRDGL PIIELVNYVE DRIIEEGGRP    50
AFPCNVSVNE VTAHYTSPPG DDSVIGDGDL VKLDLGAHVD GFIADTAITV 100
PVGDVDDKCH QMMDAAREAL ENAISTIRAG VEVGEIGRVI EETIHSHGMN 150
PVSNLTGHSM ERWILHSGLS IPNINERNTH QLEEGDVLAI EPFATDGVGL 200
VTDMPQTYIF RFLRERPLRL VHARRVLGKI REEYHALPFA QRWLEEYFDA 250
KRLNASMRML IQSRAIYPYH VLREKSGAMV AQWEHTVIVE EDGCTVITE 299
Length:299
Mass (Da):33,431
Last modified:January 1, 1998 - v1
Checksum:iDA37006A06638167
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85776.1.
PIRiC69039.
RefSeqiNP_276415.1. NC_000916.1.
WP_010876911.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85776; AAB85776; MTH_1296.
GeneIDi1471013.
KEGGimth:MTH1296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85776.1 .
PIRi C69039.
RefSeqi NP_276415.1. NC_000916.1.
WP_010876911.1. NC_000916.1.

3D structure databases

ProteinModelPortali O27355.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH1296.

Protein family/group databases

MEROPSi M24.035.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85776 ; AAB85776 ; MTH_1296 .
GeneIDi 1471013.
KEGGi mth:MTH1296.

Phylogenomic databases

eggNOGi COG0024.
KOi K01265.
OMAi NNCVAHY.

Enzyme and pathway databases

BioCyci MTHE187420:GJNM-1298-MONOMER.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPi MF_01975. MetAP_2_arc.
InterProi IPR028595. MetAP_archaeal.
IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR10804:SF9. PTHR10804:SF9. 1 hit.
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 2 hits.
TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
PROSITEi PS01202. MAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiMAP2_METTH
AccessioniPrimary (citable) accession number: O27355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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