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O27276

- DPOL1_METTH

UniProt

O27276 - DPOL1_METTH

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Protein

DNA polymerase PolB subunit 1

Gene
polB1, MTH_1208
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' exonuclease activity.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).1 Publication

Enzyme regulationi

DNA polymerase is inhibited by replication protein A (RPA), while 3'-5' exonuclease activity is not. Polymerase inhibition can be overcome by replication factor C (RFC) and PCNA.1 Publication

Temperature dependencei

Optimum temperature for DNA polymerase is 60 degrees Celsius, functional between 60 and 80 degrees Celsius. Exonuclease activity is higher at 70 than 50 degrees Celsius.1 Publication

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. exonuclease activity Source: UniProtKB-KW
  4. nucleotide binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1210-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase PolB subunit 1 (EC:2.7.7.7)
Gene namesi
Name:polB1
Ordered Locus Names:MTH_1208
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586DNA polymerase PolB subunit 1PRO_0000046477Add
BLAST

Interactioni

Subunit structurei

Formed of a complex between PolB1 and PolB2; the exonuclease activity is associated with subunit 1. Probably binds replication protein A (RPA).1 Publication

Protein-protein interaction databases

STRINGi187420.MTH1208.

Structurei

3D structure databases

ProteinModelPortaliO27276.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0417.
KOiK02319.
OMAiHFEADIP.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27276-1 [UniParc]FASTAAdd to Basket

« Hide

MEDYRMVLLD IDYVTVDEVP VIRLFGKDKS GGNEPIIAHD RSFRPYIYAI    50
PTDLDECLRE LEELELEKLE VKEMRDLGRP TEVIRIEFRH PQDVPKIRDR 100
IRDLESVRDI REHDIPFYRR YLIDKSIVPM EELEFQGVEV DSAPSVTTDV 150
RTVEVTGRVQ STGSGAHGLD ILSFDIEVRN PHGMPDPEKD EIVMIGVAGN 200
MGYESVISTA GDHLDFVEVV EDERELLERF AEIVIDKKPD ILVGYNSDNF 250
DFPYITRRAA ILGAELDLGW DGSKIRTMRR GFANATAIKG TVHVDLYPVM 300
RRYMNLDRYT LERVYQELFG EEKIDLPGDR LWEYWDRDEL RDELFRYSLD 350
DVVATHRIAE KILPLNLELT RLVGQPLFDI SRMATGQQAE WFLVRKAYQY 400
GELVPNKPSQ SDFSSRRGRR AVGGYVKEPE KGLHENIVQF DFRSLYPSII 450
ISKNISPDTL TDDEESECYV APEYGYRFRK SPRGFVPSVI GEILSERVRI 500
KEEMKGSDDP MERKILNVQQ EALKRLANTM YGVYGYSRFR WYSMECAEAI 550
TAWGRDYIKK TIKTAEEFGF HTVYADTDGF YATYRG 586
Length:586
Mass (Da):67,966
Last modified:January 1, 1998 - v1
Checksum:iD406B5BC399B951A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85697.1.
PIRiC69028.
RefSeqiNP_276336.1. NC_000916.1.
WP_010876832.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85697; AAB85697; MTH_1208.
GeneIDi1471616.
KEGGimth:MTH1208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000666 Genomic DNA. Translation: AAB85697.1 .
PIRi C69028.
RefSeqi NP_276336.1. NC_000916.1.
WP_010876832.1. NC_000916.1.

3D structure databases

ProteinModelPortali O27276.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 187420.MTH1208.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85697 ; AAB85697 ; MTH_1208 .
GeneIDi 1471616.
KEGGi mth:MTH1208.

Phylogenomic databases

eggNOGi COG0417.
KOi K02319.
OMAi HFEADIP.

Enzyme and pathway databases

BioCyci MTHE187420:GJNM-1210-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view ]
PRINTSi PR00106. DNAPOLB.
SMARTi SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 2 hits.
PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. "Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH."
    Kelman Z., Pietrokovski S., Hurwitz J.
    J. Biol. Chem. 274:28751-28761(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A DNA POLYMERASE, FUNCTION AS AN EXONUCLEASE, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH POLB2 AND RPA, SUBUNIT.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiDPOL1_METTH
AccessioniPrimary (citable) accession number: O27276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In some Methanobacteriaceae the PolB protein is split over 2 genes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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