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Protein

DNA polymerase PolB subunit 1

Gene

polB1

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' exonuclease activity.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).1 Publication

Enzyme regulationi

DNA polymerase is inhibited by replication protein A (RPA), while 3'-5' exonuclease activity is not. Polymerase inhibition can be overcome by replication factor C (RFC) and PCNA.1 Publication

Temperature dependencei

Optimum temperature for DNA polymerase is 60 degrees Celsius, functional between 60 and 80 degrees Celsius. Exonuclease activity is higher at 70 than 50 degrees Celsius.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1210-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase PolB subunit 1 (EC:2.7.7.7)
Gene namesi
Name:polB1
Ordered Locus Names:MTH_1208
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586DNA polymerase PolB subunit 1PRO_0000046477Add
BLAST

Interactioni

Subunit structurei

Formed of a complex between PolB1 and PolB2; the exonuclease activity is associated with subunit 1. Probably binds replication protein A (RPA).1 Publication

Protein-protein interaction databases

STRINGi187420.MTH1208.

Structurei

3D structure databases

ProteinModelPortaliO27276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Phylogenomic databases

eggNOGiCOG0417.
KOiK02319.
OMAiKRLANTM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDYRMVLLD IDYVTVDEVP VIRLFGKDKS GGNEPIIAHD RSFRPYIYAI
60 70 80 90 100
PTDLDECLRE LEELELEKLE VKEMRDLGRP TEVIRIEFRH PQDVPKIRDR
110 120 130 140 150
IRDLESVRDI REHDIPFYRR YLIDKSIVPM EELEFQGVEV DSAPSVTTDV
160 170 180 190 200
RTVEVTGRVQ STGSGAHGLD ILSFDIEVRN PHGMPDPEKD EIVMIGVAGN
210 220 230 240 250
MGYESVISTA GDHLDFVEVV EDERELLERF AEIVIDKKPD ILVGYNSDNF
260 270 280 290 300
DFPYITRRAA ILGAELDLGW DGSKIRTMRR GFANATAIKG TVHVDLYPVM
310 320 330 340 350
RRYMNLDRYT LERVYQELFG EEKIDLPGDR LWEYWDRDEL RDELFRYSLD
360 370 380 390 400
DVVATHRIAE KILPLNLELT RLVGQPLFDI SRMATGQQAE WFLVRKAYQY
410 420 430 440 450
GELVPNKPSQ SDFSSRRGRR AVGGYVKEPE KGLHENIVQF DFRSLYPSII
460 470 480 490 500
ISKNISPDTL TDDEESECYV APEYGYRFRK SPRGFVPSVI GEILSERVRI
510 520 530 540 550
KEEMKGSDDP MERKILNVQQ EALKRLANTM YGVYGYSRFR WYSMECAEAI
560 570 580
TAWGRDYIKK TIKTAEEFGF HTVYADTDGF YATYRG
Length:586
Mass (Da):67,966
Last modified:January 1, 1998 - v1
Checksum:iD406B5BC399B951A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85697.1.
PIRiC69028.
RefSeqiNP_276336.1. NC_000916.1.
WP_010876832.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85697; AAB85697; MTH_1208.
GeneIDi1471616.
KEGGimth:MTH1208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85697.1.
PIRiC69028.
RefSeqiNP_276336.1. NC_000916.1.
WP_010876832.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85697; AAB85697; MTH_1208.
GeneIDi1471616.
KEGGimth:MTH1208.

Phylogenomic databases

eggNOGiCOG0417.
KOiK02319.
OMAiKRLANTM.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1210-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. "Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH."
    Kelman Z., Pietrokovski S., Hurwitz J.
    J. Biol. Chem. 274:28751-28761(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A DNA POLYMERASE, FUNCTION AS AN EXONUCLEASE, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH POLB2 AND RPA, SUBUNIT.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiDPOL1_METTH
AccessioniPrimary (citable) accession number: O27276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: July 22, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In some Methanobacteriaceae the PolB protein is split over 2 genes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.