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O27276

- DPOL1_METTH

UniProt

O27276 - DPOL1_METTH

Protein

DNA polymerase PolB subunit 1

Gene

polB1

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    In addition to polymerase activity, this DNA polymerase exhibits 3'-5' exonuclease activity.1 Publication

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).1 Publication

    Enzyme regulationi

    DNA polymerase is inhibited by replication protein A (RPA), while 3'-5' exonuclease activity is not. Polymerase inhibition can be overcome by replication factor C (RFC) and PCNA.1 Publication

    Temperature dependencei

    Optimum temperature for DNA polymerase is 60 degrees Celsius, functional between 60 and 80 degrees Celsius. Exonuclease activity is higher at 70 than 50 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. exonuclease activity Source: UniProtKB-KW
    4. nucleotide binding Source: InterPro

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-1210-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase PolB subunit 1 (EC:2.7.7.7)
    Gene namesi
    Name:polB1
    Ordered Locus Names:MTH_1208
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 586586DNA polymerase PolB subunit 1PRO_0000046477Add
    BLAST

    Interactioni

    Subunit structurei

    Formed of a complex between PolB1 and PolB2; the exonuclease activity is associated with subunit 1. Probably binds replication protein A (RPA).1 Publication

    Protein-protein interaction databases

    STRINGi187420.MTH1208.

    Structurei

    3D structure databases

    ProteinModelPortaliO27276.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated

    Phylogenomic databases

    eggNOGiCOG0417.
    KOiK02319.
    OMAiHFEADIP.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 1 hit.
    [Graphical view]
    PRINTSiPR00106. DNAPOLB.
    SMARTiSM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 2 hits.
    PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O27276-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDYRMVLLD IDYVTVDEVP VIRLFGKDKS GGNEPIIAHD RSFRPYIYAI    50
    PTDLDECLRE LEELELEKLE VKEMRDLGRP TEVIRIEFRH PQDVPKIRDR 100
    IRDLESVRDI REHDIPFYRR YLIDKSIVPM EELEFQGVEV DSAPSVTTDV 150
    RTVEVTGRVQ STGSGAHGLD ILSFDIEVRN PHGMPDPEKD EIVMIGVAGN 200
    MGYESVISTA GDHLDFVEVV EDERELLERF AEIVIDKKPD ILVGYNSDNF 250
    DFPYITRRAA ILGAELDLGW DGSKIRTMRR GFANATAIKG TVHVDLYPVM 300
    RRYMNLDRYT LERVYQELFG EEKIDLPGDR LWEYWDRDEL RDELFRYSLD 350
    DVVATHRIAE KILPLNLELT RLVGQPLFDI SRMATGQQAE WFLVRKAYQY 400
    GELVPNKPSQ SDFSSRRGRR AVGGYVKEPE KGLHENIVQF DFRSLYPSII 450
    ISKNISPDTL TDDEESECYV APEYGYRFRK SPRGFVPSVI GEILSERVRI 500
    KEEMKGSDDP MERKILNVQQ EALKRLANTM YGVYGYSRFR WYSMECAEAI 550
    TAWGRDYIKK TIKTAEEFGF HTVYADTDGF YATYRG 586
    Length:586
    Mass (Da):67,966
    Last modified:January 1, 1998 - v1
    Checksum:iD406B5BC399B951A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85697.1.
    PIRiC69028.
    RefSeqiNP_276336.1. NC_000916.1.
    WP_010876832.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85697; AAB85697; MTH_1208.
    GeneIDi1471616.
    KEGGimth:MTH1208.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000666 Genomic DNA. Translation: AAB85697.1 .
    PIRi C69028.
    RefSeqi NP_276336.1. NC_000916.1.
    WP_010876832.1. NC_000916.1.

    3D structure databases

    ProteinModelPortali O27276.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 187420.MTH1208.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB85697 ; AAB85697 ; MTH_1208 .
    GeneIDi 1471616.
    KEGGi mth:MTH1208.

    Phylogenomic databases

    eggNOGi COG0417.
    KOi K02319.
    OMAi HFEADIP.

    Enzyme and pathway databases

    BioCyci MTHE187420:GJNM-1210-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 1 hit.
    [Graphical view ]
    PRINTSi PR00106. DNAPOLB.
    SMARTi SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 2 hits.
    PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
    2. "Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH."
      Kelman Z., Pietrokovski S., Hurwitz J.
      J. Biol. Chem. 274:28751-28761(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A DNA POLYMERASE, FUNCTION AS AN EXONUCLEASE, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH POLB2 AND RPA, SUBUNIT.
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiDPOL1_METTH
    AccessioniPrimary (citable) accession number: O27276
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In some Methanobacteriaceae the PolB protein is split over 2 genes.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3