O27272 (PUR5_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	MTH_1204

Organism

Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanobacterium thermoformicicum

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741_A
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741_A
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741_A		PRO_0000148284

Sequences

	Sequence		Length	Mass (Da)
	O27272 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5C6AA2B6562E0E17	FASTA	338	35,983
	10 20 30 40 50 60 MVTYSESGVD IDLEELTVSS LTSRLSDTLK YCDVITGAGH FAALVRMGDV AIAMSTDGVG 70 80 90 100 110 120 SKILVAEMMN RYDTVGIDCI AMVVNDILCV GARPAALVDY LAVERPDPEV AGEIGKGLAM 130 140 150 160 170 180 GAEMARVAII GGETASLPGI IRNLDLAATG IGFVDVDRII TGEDVSPGDT VIGLESSGIH 190 200 210 220 230 240 SNGLSLARRV FFDELELSPE DEMPGSSSSV GDELLRPTRI YVEPIMELIE SDVDVHGLAH 250 260 270 280 290 300 ITGGGFGNLK RLRKDVGYRL DSLPEPQQIF RVIHEAGVDI REMYRVFNMG VGFCAVVAPD 310 320 330 DVDEALSILG DRAHPIGEVT DRGSEVELEA CTGETIKL

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85693.1.
PIR	G69027.
RefSeq	NP_276332.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27272.
ModBase	Search...
Protein-protein interaction databases
STRING	187420.MTH1204.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB85693; AAB85693; MTH_1204.
GeneID	1471612.
KEGG	mth:MTH1204.
Phylogenomic databases
eggNOG	COG0150.
KO	K01933.
OMA	IDMIAMN.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	MTHE187420:GJNM-1206-MONOMER.
UniPathway	UPA00074; UER00129.
Family and domain databases
HAMAP	MF_00741_A. AIRS_A.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_METTH

Accession

Primary (citable) accession number: O27272

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program