O27272 (PUR5_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	MTH_1204

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_A
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_A.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_A		PRO_0000148284

Sequences

	Sequence		Length	Mass (Da)
	O27272 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5C6AA2B6562E0E17	FASTA	338	35,983
	10 20 30 40 50 60 MVTYSESGVD IDLEELTVSS LTSRLSDTLK YCDVITGAGH FAALVRMGDV AIAMSTDGVG 70 80 90 100 110 120 SKILVAEMMN RYDTVGIDCI AMVVNDILCV GARPAALVDY LAVERPDPEV AGEIGKGLAM 130 140 150 160 170 180 GAEMARVAII GGETASLPGI IRNLDLAATG IGFVDVDRII TGEDVSPGDT VIGLESSGIH 190 200 210 220 230 240 SNGLSLARRV FFDELELSPE DEMPGSSSSV GDELLRPTRI YVEPIMELIE SDVDVHGLAH 250 260 270 280 290 300 ITGGGFGNLK RLRKDVGYRL DSLPEPQQIF RVIHEAGVDI REMYRVFNMG VGFCAVVAPD 310 320 330 DVDEALSILG DRAHPIGEVT DRGSEVELEA CTGETIKL

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85693.1.
PIR	G69027.
RefSeq	NP_276332.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27272.
ModBase	Search...
Protein-protein interaction databases
STRING	O27272.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471612.
GenomeReviews	Gene locus MTH_1204 in contig AE000666_GR.
KEGG	mth:MTH1204.
NMPDR	fig|187420.1.peg.1187.
Phylogenomic databases
eggNOG	arNOG04189.
HOGENOM	HBG531222.
OMA	GIDMIAM.
PhylomeDB	O27272.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1204-MONOMER.
Family and domain databases
HAMAP	MF_00741_A. AIRS_A. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_METTH

Accession

Primary (citable) accession number: O27272

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program