O27250 (COMB_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-phosphosulfolactate phosphatase
EC=3.1.3.71

Gene names

Name:	comB
Ordered Locus Names:	MTH_1182

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(2R)-2-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate. HAMAP MF_00490
Cofactor	Magnesium By similarity. HAMAP MF_00490
Pathway	Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 2/4. HAMAP MF_00490
Sequence similarities	Belongs to the ComB family.

Ontologies

Keywords
Biological process	Coenzyme M biosynthesis
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	coenzyme M biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-phosphosulfolactate phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	2-phosphosulfolactate phosphatase HAMAP MF_00490		PRO_0000081460

Sequences

	Sequence		Length	Mass (Da)
	O27250 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C83853251C5804A7	FASTA	226	24,807
	10 20 30 40 50 60 MAMRIRLSFE RPEGSGLCIM VDLLRASATI TAALDRFREV IPVADIEEAM EYSRKGYLVA 70 80 90 100 110 120 GERGGETLPG FIANSPLEVK NYRGDVLVLT TSNGTRILES VKSDALVGCL NNLDAVAEAA 130 140 150 160 170 180 RELSDEVEVV MAGVNGRFAI EDFLCAGEII AAIDGEMDEY AEASVLAVQD RSLVDDAIRR 190 200 210 220 SRSAERLGGL GFMDDVEYCI KRNITGNVPV YRDGRIELME EIRRLH

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85671.1.
PIR	F69024.
RefSeq	NP_276310.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27250.
ModBase	Search...
Protein-protein interaction databases
STRING	O27250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471590.
GenomeReviews	Gene locus MTH_1182 in contig AE000666_GR.
KEGG	mth:MTH1182.
NMPDR	fig|187420.1.peg.1165.
Phylogenomic databases
eggNOG	arNOG10382.
HOGENOM	HBG633644.
OMA	VPCHREN.
PhylomeDB	O27250.
ProtClustDB	CLSK798095.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1182-MONOMER.
Family and domain databases
HAMAP	MF_00490. ComB. [Tree]
InterPro	IPR005238. 2-PSlactate_phosphatase. IPR022995. Pase_ComB. [Graphical view]
Gene3D	G3DSA:3.90.1560.10. 2-PSlactate_phosphatase. 1 hit.
KO	K05979.
Pfam	PF04029. 2-ph_phosp. 1 hit. [Graphical view]
SUPFAM	SSF142823. SSF142823. 1 hit.
TIGRFAMs	TIGR00298. TIGR00298. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COMB_METTH

Accession

Primary (citable) accession number: O27250

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program