O27233 (MCRG_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methyl-coenzyme M reductase I subunit gamma
Short name=MCR I gamma
EC=2.8.4.1

Alternative name(s):
Coenzyme-B sulfoethylthiotransferase gamma

Gene names

Name:	mcrG
Ordered Locus Names:	MTH_1165

Organism

Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanobacterium thermoformicicum

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.
Catalytic activity	Methyl-CoM + CoB = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.
Developmental stage	There are two MCR complexes in this bacteria. MCR II is expressed in the early growth phase. Late growth cells contains mostly MCR I.
Sequence caution	The sequence AAB85654.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 249	248	Methyl-coenzyme M reductase I subunit gamma		PRO_0000147478

Sequences

	Sequence		Length	Mass (Da)
	O27233 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 95C9C36D111BA5C6	FASTA	249	28,717
	10 20 30 40 50 60 MAQYYPGTSK VAQNRRNFCN PEYELEKLRE ISDEDVVKIL GHRAPGEEYP SVHPPLEEMD 70 80 90 100 110 120 EPEDAIREMV EPIDGAKAGD RVRYIQFTDS MYFAPAQPYV RSRAYLCRYR GADAGTLSGR 130 140 150 160 170 180 QIIETRERDL EKISKELLET EFFDPARSGV RGKSVHGHSL RLDEDGMMFD MLRRQIYNKD 190 200 210 220 230 240 TGRVEMVKNQ IGDELDEPVD LGEPLDEETL MNKTTIYRVD GEAYRDDTDA VEIMQRIHVL RSQGGYNPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H." Pihl T.D., Sharma S., Reeve J.N. J. Bacteriol. 176:6384-6391(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
[3]	"Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta H." Rospert S., Linder D., Ellermann J., Thauer R.K. Eur. J. Biochem. 194:871-877(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10036 Genomic DNA. Translation: AAA73444.1. AE000666 Genomic DNA. Translation: AAB85654.1. Different initiation.
PIR	C69022.
RefSeq	NP_276293.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27233.
SMR	O27233. Positions 2-247.
ModBase	Search...
Protein-protein interaction databases
IntAct	O27233. 1 interaction.
STRING	187420.MTH1165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB85654; AAB85654; MTH_1165.
GeneID	1471573.
KEGG	mth:MTH1165.
Phylogenomic databases
eggNOG	COG4057.
KO	K00402.
OMA	MAQYYPG.
ProtClustDB	CLSK876060.
Enzyme and pathway databases
BioCyc	MetaCyc:MCRGMAUTO-MONOMER. MTHE187420:GJNM-1167-MONOMER.
UniPathway	UPA00646; UER00699.
Family and domain databases
Gene3D	3.90.320.20. 1 hit.
InterPro	IPR009024. Me_CoM_Rdtase_Fd-like_fold. IPR003178. Me_CoM_Rdtase_gsu. [Graphical view]
Pfam	PF02240. MCR_gamma. 1 hit. [Graphical view]
PIRSF	PIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDom	PD005845. Me_CoM_Rdtase_gsu. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF55088. MCR_fer_like. 1 hit.
TIGRFAMs	TIGR03259. met_CoM_red_gam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRG_METTH

Accession

Primary (citable) accession number: O27233
Secondary accession number(s): Q50492

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program