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Protein

Methyl-coenzyme M reductase I subunit gamma

Gene

mcrG

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activityi

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Pathway:imethyl-coenzyme M reduction

This protein is involved in step 1 of the subpathway that synthesizes methane from methyl-coenzyme M.
Proteins known to be involved in this subpathway in this organism are:
  1. Methyl-coenzyme M reductase subunit alpha (mcrA), Methyl-coenzyme M reductase I subunit gamma (mcrG), Methyl-coenzyme M reductase I subunit beta (mcrB), Methyl-coenzyme M reductase II subunit gamma (mrtG), Methyl-coenzyme M reductase II subunit beta (mrtB), Methyl-coenzyme M reductase II subunit alpha (mrtA)
This subpathway is part of the pathway methyl-coenzyme M reduction, which is itself part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes methane from methyl-coenzyme M, the pathway methyl-coenzyme M reduction and in One-carbon metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Methanogenesis

Enzyme and pathway databases

BioCyciMetaCyc:MCRGMAUTO-MONOMER.
MTHE187420:GJNM-1167-MONOMER.
UniPathwayiUPA00646; UER00699.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-coenzyme M reductase I subunit gamma (EC:2.8.4.1)
Short name:
MCR I gamma
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase gamma
Gene namesi
Name:mcrG
Ordered Locus Names:MTH_1165
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 249248Methyl-coenzyme M reductase I subunit gammaPRO_0000147478Add
BLAST

Expressioni

Developmental stagei

There are two MCR complexes in this bacteria. MCR II is expressed in the early growth phase. Late growth cells contains mostly MCR I.

Interactioni

Subunit structurei

Hexamer of two alpha, two beta, and two gamma chains.

Protein-protein interaction databases

IntActiO27233. 1 interaction.
STRINGi187420.MTH1165.

Structurei

3D structure databases

ProteinModelPortaliO27233.
SMRiO27233. Positions 2-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG4057.
KOiK00402.
OMAiKRTTIYR.

Family and domain databases

Gene3Di3.90.320.20. 1 hit.
InterProiIPR009024. Me_CoM_Rdtase_Fd-like_fold.
IPR003178. Me_CoM_Rdtase_gsu.
[Graphical view]
PfamiPF02240. MCR_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDomiPD005845. Me_CoM_Rdtase_gsu. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55088. SSF55088. 1 hit.
TIGRFAMsiTIGR03259. met_CoM_red_gam. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O27233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQYYPGTSK VAQNRRNFCN PEYELEKLRE ISDEDVVKIL GHRAPGEEYP
60 70 80 90 100
SVHPPLEEMD EPEDAIREMV EPIDGAKAGD RVRYIQFTDS MYFAPAQPYV
110 120 130 140 150
RSRAYLCRYR GADAGTLSGR QIIETRERDL EKISKELLET EFFDPARSGV
160 170 180 190 200
RGKSVHGHSL RLDEDGMMFD MLRRQIYNKD TGRVEMVKNQ IGDELDEPVD
210 220 230 240
LGEPLDEETL MNKTTIYRVD GEAYRDDTDA VEIMQRIHVL RSQGGYNPE
Length:249
Mass (Da):28,717
Last modified:January 23, 2007 - v3
Checksum:i95C9C36D111BA5C6
GO

Sequence cautioni

The sequence AAB85654.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10036 Genomic DNA. Translation: AAA73444.1.
AE000666 Genomic DNA. Translation: AAB85654.1. Different initiation.
PIRiC69022.
RefSeqiNP_276293.1. NC_000916.1.
WP_010876789.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85654; AAB85654; MTH_1165.
GeneIDi1471573.
KEGGimth:MTH1165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10036 Genomic DNA. Translation: AAA73444.1.
AE000666 Genomic DNA. Translation: AAB85654.1. Different initiation.
PIRiC69022.
RefSeqiNP_276293.1. NC_000916.1.
WP_010876789.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27233.
SMRiO27233. Positions 2-247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO27233. 1 interaction.
STRINGi187420.MTH1165.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85654; AAB85654; MTH_1165.
GeneIDi1471573.
KEGGimth:MTH1165.

Phylogenomic databases

eggNOGiCOG4057.
KOiK00402.
OMAiKRTTIYR.

Enzyme and pathway databases

UniPathwayiUPA00646; UER00699.
BioCyciMetaCyc:MCRGMAUTO-MONOMER.
MTHE187420:GJNM-1167-MONOMER.

Family and domain databases

Gene3Di3.90.320.20. 1 hit.
InterProiIPR009024. Me_CoM_Rdtase_Fd-like_fold.
IPR003178. Me_CoM_Rdtase_gsu.
[Graphical view]
PfamiPF02240. MCR_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDomiPD005845. Me_CoM_Rdtase_gsu. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55088. SSF55088. 1 hit.
TIGRFAMsiTIGR03259. met_CoM_red_gam. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H."
    Pihl T.D., Sharma S., Reeve J.N.
    J. Bacteriol. 176:6384-6391(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  3. "Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta H."
    Rospert S., Linder D., Ellermann J., Thauer R.K.
    Eur. J. Biochem. 194:871-877(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiMCRG_METTH
AccessioniPrimary (citable) accession number: O27233
Secondary accession number(s): Q50492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.