ID   HMD_METTH               Reviewed;         344 AA.
AC   O27211; Q50512;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   20-JAN-2009, entry version 61.
DE   RecName: Full=5,10-methenyltetrahydromethanopterin hydrogenase;
DE            EC=1.12.98.2;
DE   AltName: Full=H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase;
DE   AltName: Full=N(5),N(10)-methenyltetrahydromethanopterin hydrogenase;
DE   AltName: Full=H(2)-dependent methylene-H(4)MPT dehydrogenase;
GN   Name=hmd; Synonyms=mth; OrderedLocusNames=MTH_1142;
OS   Methanobacterium thermoautotrophicum.
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Delta H;
RX   MEDLINE=95247681; PubMed=7730278;
RA   Noelling J., Pihl T.D., Vriesema A., Reeve J.N.;
RT   "Organization and growth phase-dependent transcription of methane
RT   genes in two regions of the Methanobacterium thermoautotrophicum
RT   genome.";
RL   J. Bacteriol. 177:2460-2468(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Delta H;
RX   MEDLINE=98037514; PubMed=9371463;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K.,
RA   Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D.,
RA   Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R.,
RA   Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S.,
RA   McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M.,
RA   Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum
RT   deltaH: functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-
CC       H(4)MPT(+) to methylene-H(4)MPT (By similarity).
CC   -!- CATALYTIC ACTIVITY: H(2) + 5,10-methenyltetrahydromethanopterin =
CC       H(+) + 5,10-methylenetetrahydromethanopterin.
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from carbone
CC       dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT
CC       (hydrogen route): step 1/1.
CC   -!- SIMILARITY: Belongs to the HMD family.
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DR   EMBL; U19363; AAA87423.1; -; Genomic_DNA.
DR   EMBL; AE000666; AAB85631.1; ALT_INIT; Genomic_DNA.
DR   PIR; B69019; B69019.
DR   RefSeq; NP_276270.1; -.
DR   GeneID; 1471550; -.
DR   GenomeReviews; AE000666_GR; MTH_1142.
DR   KEGG; mth:MTH1142; -.
DR   NMPDR; fig|187420.1.peg.1125; -.
DR   HOGENOM; O27211; -.
DR   BioCyc; MetaCyc:HMDMAUTO-MON; -.
DR   BioCyc; MTHE187420:MTH1142-MON; -.
DR   BRENDA; 1.12.98.2; 270.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0018537; F:coenzyme F420-dependent N5,N10-methenyltetr...; IEA:InterPro.
DR   GO; GO:0047068; F:N5,N10-methenyltetrahydromethanopterin hydr...; IEA:HAMAP.
DR   GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01090; -; 1.
DR   InterPro; IPR004889; HMD.
DR   InterPro; IPR010062; HMD_sub.
DR   Pfam; PF03201; HMD; 1.
DR   TIGRFAMs; TIGR01723; hmd_TIGR; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methanogenesis; One-carbon metabolism;
KW   Oxidoreductase.
FT   CHAIN         1    344       5,10-methenyltetrahydromethanopterin
FT                                hydrogenase.
FT                                /FTId=PRO_0000218511.
SQ   SEQUENCE   344 AA;  37576 MW;  12E600166BB33E48 CRC64;
     MKLAILGAGC YRTHAASGIT NFSRACEVAE MVGKPEIAMT HSTITMGAEL KELAGVDEVV
     VADPVFDNQF TVIDDFAYED VIEAHKEDPE KIMPQIREKV NEVAKELPKP PEGAIHFTHP
     EDLGFEITTD DREAVADADF IMTWFPKGDM QPGIIDKFID DIKPGAIVTH ACTIPTTKFY
     KIFEEKHGDL VTKPETLNVT SYHPGAVPEM KGQVYIAEGY ASEEAINTLF ELGQKARGNA
     YRLPAELLGP VCDMCSALTA ITYAGILSYR DSVTQVLGAP AGFAQMMAKE SLEQITALME
     KVGIDKMEEH LDPGALLGTA DSMNFGASAD ILPTVFEILE KRKK
//