Reviewed, UniProtKB/Swiss-Prot O27211 (HMD_METTH)

Names and origin

Protein names

Recommended name:
    5,10-methenyltetrahydromethanopterin hydrogenase
    EC=1.12.98.2
Alternative name(s):
    H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase
    N(5),N(10)-methenyltetrahydromethanopterin hydrogenase
    H(2)-dependent methylene-H(4)MPT dehydrogenase

Gene names

Name:	hmd
Synonyms:	mth
Ordered Locus Names:	MTH_1142

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT By similarity.
Catalytic activity	H2 + 5,10-methenyltetrahydromethanopterin = H+ + 5,10-methylenetetrahydromethanopterin. HAMAP MF_01090
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT (hydrogen route): step 1/1. HAMAP MF_01090
Sequence similarities	Belongs to the HMD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis, from carbon dioxide Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N5,N10-methenyltetrahydromethanopterin hydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	5,10-methenyltetrahydromethanopterin hydrogenase HAMAP MF_01090		PRO_0000218511

Sequences

	Sequence		Length	Mass (Da)
	O27211-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 12E600166BB33E48	FASTA	344	37,576
	10 20 30 40 50 60 MKLAILGAGC YRTHAASGIT NFSRACEVAE MVGKPEIAMT HSTITMGAEL KELAGVDEVV 70 80 90 100 110 120 VADPVFDNQF TVIDDFAYED VIEAHKEDPE KIMPQIREKV NEVAKELPKP PEGAIHFTHP 130 140 150 160 170 180 EDLGFEITTD DREAVADADF IMTWFPKGDM QPGIIDKFID DIKPGAIVTH ACTIPTTKFY 190 200 210 220 230 240 KIFEEKHGDL VTKPETLNVT SYHPGAVPEM KGQVYIAEGY ASEEAINTLF ELGQKARGNA 250 260 270 280 290 300 YRLPAELLGP VCDMCSALTA ITYAGILSYR DSVTQVLGAP AGFAQMMAKE SLEQITALME 310 320 330 340 KVGIDKMEEH LDPGALLGTA DSMNFGASAD ILPTVFEILE KRKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and growth phase-dependent transcription of methane genes in two regions of the Methanobacterium thermoautotrophicum genome." Noelling J., Pihl T.D., Vriesema A., Reeve J.N. J. Bacteriol. 177:2460-2468(1995) [PubMed: 7730278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Delta H.
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U19363 Genomic DNA. Translation: AAA87423.1. AE000666 Genomic DNA. Translation: AAB85631.1. Different initiation.
PIR	B69019.
RefSeq	NP_276270.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1471550.
GenomeReviews	Gene locus MTH_1142 in contig AE000666_GR.
KEGG	mth:MTH1142.
NMPDR	fig|187420.1.peg.1125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O27211.
Enzyme and pathway databases
BioCyc	MetaCyc:HMDMAUTO-MON. MTHE187420:MTH1142-MON.
BRENDA	1.12.98.2. 270.
Family and domain databases
HAMAP	MF_01090. [Tree]
InterPro	IPR004889. HMD. IPR010062. HMD_sub. [Graphical view]
Pfam	PF03201. HMD. 1 hit. [Graphical view]
TIGRFAMs	TIGR01723. hmd_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HMD_METTH

Accession

Primary (citable) accession number: O27211
Secondary accession number(s): Q50512

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	January 20, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)