O27190 (PPSA_METTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phosphoenolpyruvate synthase
Short name=PEP synthase
EC=2.7.9.2

Alternative name(s):
Pyruvate, water dikinase

Gene names

Name:	ppsA
Ordered Locus Names:	MTH_1118

Organism

Methanobacterium thermoautotrophicum (strain Delta H)

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate By similarity.
Catalytic activity	ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate.
Cofactor	Magnesium By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Domain	The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site By similarity.
Miscellaneous	The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain By similarity.
Sequence similarities	Belongs to the PEP-utilizing enzyme family.
Caution	Lacks some of the catalytic-important sites due to premature sequence termination compared to the close ortholog in M.jannaschii. The missing 250 amino acids or so may be retrieved through a serie of sequence corrections.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	Probable phosphoenolpyruvate synthase		PRO_0000147042
Sites
	Active site	424	1	Tele-phosphohistidine intermediate By similarity
	Metal binding	661	1	Magnesium By similarity
	Binding site	517	1	Substrate By similarity
	Binding site	564	1	Substrate By similarity
	Binding site	661	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O27190 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 44A76335F01798F0	FASTA	684	75,619
	10 20 30 40 50 60 MTGARNWLNK PLIFFKFNNH HTASPGDKMV KYVAFFEELG KDDVGIAGGK GANLGELTQA 70 80 90 100 110 120 GIPVPPGFVV TAATYDKFMT DTGLQPVVME MLENLDVNDT KELQRVSAEI KDIITSTEVP 130 140 150 160 170 180 EDIQTLIIES YNALCQRIGK DDVYVAIRSS ATAEDLPEAS FAGQQDTFLN IRGAEDVLDY 190 200 210 220 230 240 VRRCWASLFE ARAIFYREEN NFDHSKVYIA VVVQEMVDAE KAGVMFTVHP STGEDRILIE 250 260 270 280 290 300 GSWGLGEAVV SGSVTPDTYW VDKGTGKLLE FTVGEKNIMF TREDGRTVKK EVPPELRNKR 310 320 330 340 350 360 VLSDGEIAAL AEMGRRIQDH YGSPQDTEWA IMDGDVYMLQ SRPITTLGEA TEETEVKSRE 370 380 390 400 410 420 ILVKGLGASP GLASGRVKII REIHELDKIQ IGDILVTVMT TPDMVPAMKR ASGIITDEGG 430 440 450 460 470 480 VTCHAAIVSR ELGIPCVVGT GNATEVLKEN QVVSIDGNRG LVYEGSVIEG EKKEAEAETV 490 500 510 520 530 540 TVESPLLTVT EVKVNVSMPE AARKAAATGA DGVGLLRTEH MMLTTGVHPR KFIEEGREDE 550 560 570 580 590 600 LVNTLAENIL KVADEFYPRP VWYRTLDAPT DEFKTLEGGE NEPYEHNPML GWRGIRRELD 610 620 630 640 650 660 EPEILRAEFR AIKKLHEQGY TNIGIMIPLV QHPDELRKAK MIAEEAGLKP HRDVEFGIMV 670 680 ETPAAALIIE DFIEEGIDFV RLEP

References

[1]

"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000666 Genomic DNA. Translation: AAB85607.1.
PIR	G69015.
RefSeq	NP_276246.1. NC_000916.1.
3D structure databases
ProteinModelPortal	O27190.
ModBase	Search...
Protein-protein interaction databases
STRING	O27190.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1471526.
GenomeReviews	Gene locus MTH_1118 in contig AE000666_GR.
KEGG	mth:MTH1118.
NMPDR	fig|187420.1.peg.1101.
Phylogenomic databases
eggNOG	arNOG04015.
HOGENOM	HBG284863.
OMA	DIITSTE.
PhylomeDB	O27190.
ProtClustDB	CLSK2750506.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1118-MONOMER.
Family and domain databases
InterPro	IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR008279. PEP-util_enz_mobile_dom. IPR006319. PEP_synth. IPR018274. PEP_util_AS. IPR000121. PEP_util_C. IPR002192. PPDK_PEP-bd. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K01007.
Pfam	PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. PF01326. PPDK_N. 1 hit. [Graphical view]
SUPFAM	SSF52009. PEP_mobile. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01418. PEP_synth. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. False negative. PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPSA_METTH

Accession

Primary (citable) accession number: O27190

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program