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Protein

Elongation factor 1-alpha

Gene

tuf

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1060-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alphaUniRule annotation
Short name:
EF-1-alphaUniRule annotation
Alternative name(s):
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:MTH_1058
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Elongation factor 1-alphaPRO_0000090985Add
BLAST

Proteomic databases

PRIDEiO27132.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
leuSO275523EBI-7963138,EBI-7963108

Protein-protein interaction databases

IntActiO27132. 1 interaction.
MINTiMINT-6551005.
STRINGi187420.MTH1058.

Structurei

3D structure databases

ProteinModelPortaliO27132.
SMRiO27132. Positions 5-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 211207tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1By similarity
Regioni60 – 645G2By similarity
Regioni81 – 844G3By similarity
Regioni136 – 1394G4By similarity
Regioni175 – 1773G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
KOiK03231.
OMAiVNYDQKR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEKEHMNL AFIGHVDHGK STLVGHLLLQ AGAIAEQQLA EGEDKFRFVM
60 70 80 90 100
DRLSEERERG VTIDLAHAKF ETDKYEFTIV DCPGHRDFVK NMITGASQAD
110 120 130 140 150
AAVLVVAVDD GVMPQTKEHV FLSRTLGINQ LIVAINKMDL VNYDEEKFNA
160 170 180 190 200
LKDEVAALIK TVGYKPSDVE FIPLSAFEGD NITSKSENTP WYKGKTLVEA
210 220 230 240 250
LDDLEAPEKP VDLPLRIPIQ DVYSITGVGT VPVGRVETGV LKKGENVIFE
260 270 280 290 300
PAGVSGEVKS IEMHHEMIEQ AEPGDNIGFN VRGVGKNDIR RGDVAGHLDN
310 320 330 340 350
PPKVAKEFTA QIVVLQHPGV ITVGYTPVFH CHTAQVACTF LELVQKMNPA
360 370 380 390 400
TGQVEEENPD FLKTGNAAVV KVKPTKPLVI EKIKDIPHMG RFAIRDMGQT
410
VAAGMCIDLV PAK
Length:413
Mass (Da):45,216
Last modified:January 1, 1998 - v1
Checksum:i0C8E457913A0D26D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85549.1.
PIRiF69007.
RefSeqiWP_010876684.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85549; AAB85549; MTH_1058.
GeneIDi1471466.
KEGGimth:MTH_1058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85549.1.
PIRiF69007.
RefSeqiWP_010876684.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27132.
SMRiO27132. Positions 5-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO27132. 1 interaction.
MINTiMINT-6551005.
STRINGi187420.MTH1058.

Proteomic databases

PRIDEiO27132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85549; AAB85549; MTH_1058.
GeneIDi1471466.
KEGGimth:MTH_1058.

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
KOiK03231.
OMAiVNYDQKR.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1060-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A_METTH
AccessioniPrimary (citable) accession number: O27132
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.