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Protein

Elongation factor 2

Gene

fusA

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 35GTPBy similarity8
Nucleotide bindingi94 – 98GTPBy similarity5
Nucleotide bindingi148 – 151GTPBy similarity4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:fusA
Synonyms:fus
Ordered Locus Names:MTH_1057
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000910371 – 730Elongation factor 2Add BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei597DiphthamideBy similarity1

Proteomic databases

PRIDEiO27131.

Interactioni

Protein-protein interaction databases

STRINGi187420.MTH1057.

Structurei

3D structure databases

ProteinModelPortaliO27131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 260tr-type GAdd BLAST242

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
KOiK03234.
OMAiFTGHVTR.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O27131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRAKMISK IKELMYQPEY IRNIGIVAHI DHGKTTLSDN LLAGAGMISA
60 70 80 90 100
ELAGDQRFLD FDEQEQARGI TIDAANVSMV HSYEGNEYLI NLIDTPGHVD
110 120 130 140 150
FGGDVTRAMR AVDGAVVVVC AVEGIMPQTE TVLRQALKEN VRPVLFINKV
160 170 180 190 200
DRLINELKLD ASELQERFVK IIANANKLIK NMAPEEFRDK WQVRVEDGSV
210 220 230 240 250
AFGSAYHNWA INVPIMQETG INFNDIYKYC TEDNQKELAQ KVPLHQVLLG
260 270 280 290 300
MVVEHLPSPA ESQAYRVPII WSGDLESEEG QAMLKTDPEG PLAVMVTDVS
310 320 330 340 350
IDKHAGEVAT GRVYGGAIEK GSEVFLVGSH SKARVQQVGV YMGPERVNTD
360 370 380 390 400
KVPAGNIVAI TGAKNAVAGE TICDTGRKIK AFEGLEHISE PVVTVAVEAK
410 420 430 440 450
NTKDLPKLIE VLRQVGKEDP TVRVEINEET GEHLISGMGE LHLEIIAYRI
460 470 480 490 500
NEKGVEIETS EPIVVYRETV AGTAGPVEGK SPNKHNRFYI EIEPVEASVM
510 520 530 540 550
QAIQEGKIKE GRVKGKEMAK NFIEAGMDKE EARRVWDVYE KNLFINMTRG
560 570 580 590 600
IQYLDEIKEL LMDGFESAMD NGPVAKEKVM GVKIKLMDAK IHEDAVHRGP
610 620 630 640 650
AQVLPAIRKG IFGAMMSAEP VLLEPIQKVF INVPQDYMGS ATREIQNRRG
660 670 680 690 700
QIVNMTQEGD MVTVESTVPV AEMFGFAGDI RSATEGRCLW STENAGFERL
710 720 730
PNELQHTITR EVRTRKGLSP EPYRADHYLG
Length:730
Mass (Da):80,924
Last modified:May 30, 2000 - v2
Checksum:i07549350848DE81C
GO

Sequence cautioni

The sequence AAB85548 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85548.1. Different initiation.
PIRiE69007.
RefSeqiWP_048060960.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85548; AAB85548; MTH_1057.
GeneIDi1471465.
KEGGimth:MTH_1057.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000666 Genomic DNA. Translation: AAB85548.1. Different initiation.
PIRiE69007.
RefSeqiWP_048060960.1. NC_000916.1.

3D structure databases

ProteinModelPortaliO27131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187420.MTH1057.

Proteomic databases

PRIDEiO27131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85548; AAB85548; MTH_1057.
GeneIDi1471465.
KEGGimth:MTH_1057.

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
KOiK03234.
OMAiFTGHVTR.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF2_METTH
AccessioniPrimary (citable) accession number: O27131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.