UniProtKB - O27131 (EF2_METTH)
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Protein
Elongation factor 2
Gene
fusA
Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Functioni
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 28 – 35 | GTPBy similarity | 8 | |
Nucleotide bindingi | 94 – 98 | GTPBy similarity | 5 | |
Nucleotide bindingi | 148 – 151 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
- translation elongation factor activity Source: UniProtKB-KW
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MTHE187420:G1G16-1019-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 2Short name: EF-2 |
Gene namesi | Name:fusA Synonyms:fus Ordered Locus Names:MTH_1057 |
Organismi | Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) |
Taxonomic identifieri | 187420 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091037 | 1 – 730 | Elongation factor 2Add BLAST | 730 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 597 | DiphthamideBy similarity | 1 |
Proteomic databases
PRIDEi | O27131 |
Structurei
3D structure databases
ProteinModelPortali | O27131 |
SMRi | O27131 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 260 | tr-type GAdd BLAST | 242 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.Curated
Phylogenomic databases
eggNOGi | arCOG01559 Archaea COG0480 LUCA |
KOi | K03234 |
OMAi | EHLISGM |
OrthoDBi | POG093Z00HF |
Family and domain databases
Gene3Di | 3.30.230.102 hits |
HAMAPi | MF_00054_A EF_G_EF_2_A, 1 hit |
InterProi | View protein in InterPro IPR035647 EFG_III/V IPR000640 EFG_V-like IPR004161 EFTu-like_2 IPR031157 G_TR_CS IPR027417 P-loop_NTPase IPR020568 Ribosomal_S5_D2-typ_fold IPR014721 Ribosomal_S5_D2-typ_fold_subgr IPR005225 Small_GTP-bd_dom IPR000795 TF_GTP-bd_dom IPR009000 Transl_B-barrel_sf IPR004543 Transl_elong_EFG/EF2_arc IPR005517 Transl_elong_EFG/EF2_IV |
Pfami | View protein in Pfam PF00679 EFG_C, 1 hit PF03764 EFG_IV, 1 hit PF00009 GTP_EFTU, 1 hit PF03144 GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315 ELONGATNFCT |
SMARTi | View protein in SMART SM00838 EFG_C, 1 hit SM00889 EFG_IV, 1 hit |
SUPFAMi | SSF50447 SSF50447, 1 hit SSF52540 SSF52540, 1 hit SSF54211 SSF54211, 1 hit SSF54980 SSF54980, 2 hits |
TIGRFAMsi | TIGR00490 aEF-2, 1 hit TIGR00231 small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301 G_TR_1, 1 hit PS51722 G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O27131-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRRAKMISK IKELMYQPEY IRNIGIVAHI DHGKTTLSDN LLAGAGMISA
60 70 80 90 100
ELAGDQRFLD FDEQEQARGI TIDAANVSMV HSYEGNEYLI NLIDTPGHVD
110 120 130 140 150
FGGDVTRAMR AVDGAVVVVC AVEGIMPQTE TVLRQALKEN VRPVLFINKV
160 170 180 190 200
DRLINELKLD ASELQERFVK IIANANKLIK NMAPEEFRDK WQVRVEDGSV
210 220 230 240 250
AFGSAYHNWA INVPIMQETG INFNDIYKYC TEDNQKELAQ KVPLHQVLLG
260 270 280 290 300
MVVEHLPSPA ESQAYRVPII WSGDLESEEG QAMLKTDPEG PLAVMVTDVS
310 320 330 340 350
IDKHAGEVAT GRVYGGAIEK GSEVFLVGSH SKARVQQVGV YMGPERVNTD
360 370 380 390 400
KVPAGNIVAI TGAKNAVAGE TICDTGRKIK AFEGLEHISE PVVTVAVEAK
410 420 430 440 450
NTKDLPKLIE VLRQVGKEDP TVRVEINEET GEHLISGMGE LHLEIIAYRI
460 470 480 490 500
NEKGVEIETS EPIVVYRETV AGTAGPVEGK SPNKHNRFYI EIEPVEASVM
510 520 530 540 550
QAIQEGKIKE GRVKGKEMAK NFIEAGMDKE EARRVWDVYE KNLFINMTRG
560 570 580 590 600
IQYLDEIKEL LMDGFESAMD NGPVAKEKVM GVKIKLMDAK IHEDAVHRGP
610 620 630 640 650
AQVLPAIRKG IFGAMMSAEP VLLEPIQKVF INVPQDYMGS ATREIQNRRG
660 670 680 690 700
QIVNMTQEGD MVTVESTVPV AEMFGFAGDI RSATEGRCLW STENAGFERL
710 720 730
PNELQHTITR EVRTRKGLSP EPYRADHYLG
Sequence cautioni
The sequence AAB85548 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000666 Genomic DNA Translation: AAB85548.1 Different initiation. |
PIRi | E69007 |
RefSeqi | WP_048060960.1, NC_000916.1 |
Genome annotation databases
EnsemblBacteriai | AAB85548; AAB85548; MTH_1057 |
GeneIDi | 1471465 |
KEGGi | mth:MTH_1057 |
PATRICi | fig|187420.15.peg.1036 |
Similar proteinsi
Entry informationi
Entry namei | EF2_METTH | |
Accessioni | O27131Primary (citable) accession number: O27131 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | May 30, 2000 | |
Last modified: | March 28, 2018 | |
This is version 121 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |