fusA

Functionⁱ

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	28 – 35	GTPBy similarity	8
Nucleotide bindingⁱ	94 – 98	GTPBy similarity	5
Nucleotide bindingⁱ	148 – 151	GTPBy similarity	4

GO - Molecular functionⁱ

GTPase activity Source: InterPro
GTP binding Source: UniProtKB-KW
translation elongation factor activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Elongation factor
Biological process	Protein biosynthesis
Ligand	GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	MTHE187420:G1G16-1019-MONOMER

BioCycⁱ

MTHE187420:G1G16-1019-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Elongation factor 2 Short name: EF-2
Gene namesⁱ	Name:fusA Synonyms:fus Ordered Locus Names:MTH_1057
Organismⁱ	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifierⁱ	187420 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanothermobacter thermautotrophicus
Proteomesⁱ	UP000005223 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm By similarity

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000091037	1 – 730	Elongation factor 2Add BLAST		730

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	597	DiphthamideBy similarity		1

Proteomic databases

PRIDEⁱ	O27131

PRIDEⁱ

O27131

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	187420.MTH1057

STRINGⁱ

187420.MTH1057

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O27131
SMRⁱ	O27131
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	19 – 260	tr-type GAdd BLAST		242

Sequence similaritiesⁱ

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.Curated

Phylogenomic databases

eggNOGⁱ	arCOG01559 Archaea COG0480 LUCA
KEGG Orthology (KO) More...KOⁱ	K03234
OMAⁱ	EHLISGM
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z00HF

Family and domain databases

Gene3Dⁱ	3.30.230.102 hits
HAMAPⁱ	MF_00054_A EF_G_EF_2_A, 1 hit
InterProⁱ	View protein in InterPro IPR035647 EFG_III/V IPR000640 EFG_V-like IPR004161 EFTu-like_2 IPR031157 G_TR_CS IPR027417 P-loop_NTPase IPR020568 Ribosomal_S5_D2-typ_fold IPR014721 Ribosomal_S5_D2-typ_fold_subgr IPR005225 Small_GTP-bd_dom IPR000795 TF_GTP-bd_dom IPR009000 Transl_B-barrel_sf IPR004543 Transl_elong_EFG/EF2_arc IPR005517 Transl_elong_EFG/EF2_IV
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00679 EFG_C, 1 hit PF03764 EFG_IV, 1 hit PF00009 GTP_EFTU, 1 hit PF03144 GTP_EFTU_D2, 1 hit
PRINTSⁱ	PR00315 ELONGATNFCT
SMARTⁱ	View protein in SMART SM00838 EFG_C, 1 hit SM00889 EFG_IV, 1 hit
SUPFAMⁱ	SSF50447 SSF50447, 1 hit SSF52540 SSF52540, 1 hit SSF54211 SSF54211, 1 hit SSF54980 SSF54980, 2 hits
TIGRFAMsⁱ	TIGR00490 aEF-2, 1 hit TIGR00231 small_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS00301 G_TR_1, 1 hit PS51722 G_TR_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O27131-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSRRAKMISK IKELMYQPEY IRNIGIVAHI DHGKTTLSDN LLAGAGMISA 
        60         70         80         90        100
ELAGDQRFLD FDEQEQARGI TIDAANVSMV HSYEGNEYLI NLIDTPGHVD 
       110        120        130        140        150
FGGDVTRAMR AVDGAVVVVC AVEGIMPQTE TVLRQALKEN VRPVLFINKV 
       160        170        180        190        200
DRLINELKLD ASELQERFVK IIANANKLIK NMAPEEFRDK WQVRVEDGSV 
       210        220        230        240        250
AFGSAYHNWA INVPIMQETG INFNDIYKYC TEDNQKELAQ KVPLHQVLLG 
       260        270        280        290        300
MVVEHLPSPA ESQAYRVPII WSGDLESEEG QAMLKTDPEG PLAVMVTDVS 
       310        320        330        340        350
IDKHAGEVAT GRVYGGAIEK GSEVFLVGSH SKARVQQVGV YMGPERVNTD 
       360        370        380        390        400
KVPAGNIVAI TGAKNAVAGE TICDTGRKIK AFEGLEHISE PVVTVAVEAK 
       410        420        430        440        450
NTKDLPKLIE VLRQVGKEDP TVRVEINEET GEHLISGMGE LHLEIIAYRI 
       460        470        480        490        500
NEKGVEIETS EPIVVYRETV AGTAGPVEGK SPNKHNRFYI EIEPVEASVM 
       510        520        530        540        550
QAIQEGKIKE GRVKGKEMAK NFIEAGMDKE EARRVWDVYE KNLFINMTRG 
       560        570        580        590        600
IQYLDEIKEL LMDGFESAMD NGPVAKEKVM GVKIKLMDAK IHEDAVHRGP 
       610        620        630        640        650
AQVLPAIRKG IFGAMMSAEP VLLEPIQKVF INVPQDYMGS ATREIQNRRG 
       660        670        680        690        700
QIVNMTQEGD MVTVESTVPV AEMFGFAGDI RSATEGRCLW STENAGFERL 
       710        720        730
PNELQHTITR EVRTRKGLSP EPYRADHYLG

Length:730

Mass (Da):80,924

Last modified:May 30, 2000 - v2

Checksum:ⁱ07549350848DE81C

GO

Sequence cautionⁱ

The sequence AAB85548 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE000666 Genomic DNA Translation: AAB85548.1 Different initiation.
PIRⁱ	E69007
NCBI Reference Sequences More...RefSeqⁱ	WP_048060960.1, NC_000916.1

Genome annotation databases

EnsemblBacteriaⁱ	AAB85548; AAB85548; MTH_1057
GeneIDⁱ	1471465
KEGGⁱ	mth:MTH_1057
PATRICⁱ	fig\|187420.15.peg.1036

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
O27131	A0A223ZFA4 D9PXS8 T2GJJ8 A0A1M4MTF6	Methanothermobacter sp. EMTCatA1 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) Methanothermobacter sp. CaT2 Methanothermobacter wolfeii (Methanobacterium wolfei)	730	UniRef90_O27131	Cluster: Elongation factor 2	5

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
O27131	O59521 UPI000CE68246 F5CJ92 A0A127BBW2 F1DG84 Q9V1Z8 F5CJ90 F4HKK0 F8AFK2 F5CJ93 +764	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) Pyrococcus kukulkanii Pyrococcus sp. LMO-A31 Pyrococcus abyssi (strain GE5 / Orsay) Pyrococcus sp. (strain NA2) Pyrococcus yayanosii (strain CH1 / JCM 16557) Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) Pyrococcus woesei Pyrococcus furiosus COM1 Thermococcus chitonophagus And more	732	UniRef50_O59521	Cluster: Elongation factor 2	775

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE000666 Genomic DNA Translation: AAB85548.1 Different initiation.
PIRⁱ	E69007
NCBI Reference Sequences More...RefSeqⁱ	WP_048060960.1, NC_000916.1

3D structure databases

ProteinModelPortalⁱ	O27131
SMRⁱ	O27131
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	187420.MTH1057

STRINGⁱ

187420.MTH1057

Proteomic databases

PRIDEⁱ	O27131

PRIDEⁱ

O27131

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAB85548; AAB85548; MTH_1057
GeneIDⁱ	1471465
KEGGⁱ	mth:MTH_1057
PATRICⁱ	fig\|187420.15.peg.1036

Phylogenomic databases

eggNOGⁱ	arCOG01559 Archaea COG0480 LUCA
KEGG Orthology (KO) More...KOⁱ	K03234
OMAⁱ	EHLISGM
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z00HF

Enzyme and pathway databases

BioCycⁱ	MTHE187420:G1G16-1019-MONOMER

BioCycⁱ

MTHE187420:G1G16-1019-MONOMER

Family and domain databases

Gene3Dⁱ	3.30.230.102 hits
HAMAPⁱ	MF_00054_A EF_G_EF_2_A, 1 hit
InterProⁱ	View protein in InterPro IPR035647 EFG_III/V IPR000640 EFG_V-like IPR004161 EFTu-like_2 IPR031157 G_TR_CS IPR027417 P-loop_NTPase IPR020568 Ribosomal_S5_D2-typ_fold IPR014721 Ribosomal_S5_D2-typ_fold_subgr IPR005225 Small_GTP-bd_dom IPR000795 TF_GTP-bd_dom IPR009000 Transl_B-barrel_sf IPR004543 Transl_elong_EFG/EF2_arc IPR005517 Transl_elong_EFG/EF2_IV
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00679 EFG_C, 1 hit PF03764 EFG_IV, 1 hit PF00009 GTP_EFTU, 1 hit PF03144 GTP_EFTU_D2, 1 hit
PRINTSⁱ	PR00315 ELONGATNFCT
SMARTⁱ	View protein in SMART SM00838 EFG_C, 1 hit SM00889 EFG_IV, 1 hit
SUPFAMⁱ	SSF50447 SSF50447, 1 hit SSF52540 SSF52540, 1 hit SSF54211 SSF54211, 1 hit SSF54980 SSF54980, 2 hits
TIGRFAMsⁱ	TIGR00490 aEF-2, 1 hit TIGR00231 small_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS00301 G_TR_1, 1 hit PS51722 G_TR_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	EF2_METTH
Accessionⁱ	O27131Primary (citable) accession number: O27131
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
	Last sequence update:	May 30, 2000
	Last modified:	March 28, 2018
	This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O27131 (EF2_METTH)

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Elongation factor 2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ